Artículos de revistas
Phase Equilibria For Salt-induced Lysozyme Precipitation: Effect Of Salt Concentration And Ph
Registro en:
Chemical Engineering And Processing: Process Intensification. , v. 47, n. 6, p. 1026 - 1033, 2008.
2552701
10.1016/j.cep.2007.02.005
2-s2.0-41449106175
Autor
Popova E.
Watanabe E.O.
Filho P.A.P.
Maurer G.
Institución
Resumen
The salt-induced precipitation of lysozyme from aqueous solutions was studied at 25 °C and various pH values by cloud-point investigations, precipitation experiments (analysing the compositions of the coexisting phases) and microscopic investigations of the precipitates. Sodium sulphate as well as ammonium sulphate were used to induce the precipitation. The experimental results are discussed and used to develop a scheme of the phase equilibrium in water-rich aqueous solutions of lysozyme and either Na2SO4 or (NH4)2SO4. © 2007 Elsevier B.V. All rights reserved. 47 6 1026 1033 Asherie, N., Protein crystallisation and phase diagrams (2004) Methods, 34, pp. 266-272 Muschol, M., Rosenberger, F., Liquid-liquid phase separation in supersaturated lysozyme solutions and associated precipitate formation/crystallisation (1997) J. Chem. Phys., 107, pp. 1953-1962 Berland, C.R., Thurston, G.M., Kondo, M., Broide, M.L., Pande, J., Ogun, O., Benedek, G.B., Solid-liquid phase boundaries of lens protein solutions (1992) Proc. Natl. Acad. Sci. U.S.A., 89 (4), pp. 1214-1218 Broide, M.L., Berland, C.R., Pande, J., Ogun, O., Benedek, G.B., Binary liquid phase separation of lens protein solutions (1991) Proc. Natl. Acad. Sci. U.S.A., 88 (13), pp. 5660-5664 Moretti, J.J., Sandler, S.I., Lenhoff, A.M., Phase equilibria in the lysozyme-ammonium sulphate-water system (2000) Biotechnol. Bioeng., 70, pp. 498-506 Crompton, T.R., (1996) Determination of Anions, , Springer-Verlag, Berlin, Germany Shih, Y.-C., Prausnitz, J.M., Blanch, H.W., Some characteristics of protein precipitation by salts (1992) Biotechnol. Bioeng., 40 (10), pp. 1155-1164 Steinrauf, L.K., Preliminary X-ray data for new some crystalline forms of β-lactoglobulin and hen egg-white lysozyme (1959) Acta Cryst., 12, pp. 77-79 Salunke, D.M., Veerapandian, B., Kodandapani, R., Vijayan, M., Water-mediated transformations in protein crystals (1985) Acta Cryst. B, 41, pp. 431-436 Vaney, M.C., Broutin, I., Retailleau, P., Douangamath, A., Lafont, S., Hamiaux, C., Prange, T., Ries-Kautt, M., Structural effects of monovalent anions on polymorphic lysozyme crystals (2001) Acta Cryst. D, 57, pp. 929-940 Ewing, F., Forsythe, E., Pusey, M., Orthorhombic lysozyme solubility (1994) Acta Cryst. D, 50, pp. 424-428 Ries-Kautt, M.M., Ducruix, A., Crystallization of previously desalted lysozyme in the presence of sulfate ions (1994) Acta Cryst. D, 50, pp. 366-369 Ries-Kautt, M.M., Ducruix, A., Relative effectiveness of various ions on the solubility and crystal growth of lysozyme (1989) J. Biol. Chem., 264 (2), pp. 745-748 Forsythe, E.L., Snell, E.H., Pusey, M.L., Crystallization of chicken egg-white lysozyme from ammonium sulfate (1997) Acta Cryst. D, 53, pp. 795-797 Forsythe, E.L., Judge, R.A., Pusey, M.L., Tetragonal chicken egg white lysozyme solubility in sodium chloride solutions (1999) J. Chem. Eng. Data, 44 (3), pp. 637-640