Artículos de revistas
Genetic Variability In The G Protein Gene Of Human Respiratory Syncytial Virus Isolated From The Campinas Metropolitan Region, Brazil
Registro en:
Journal Of Medical Virology. , v. 80, n. 9, p. 1653 - 1660, 2008.
1466615
10.1002/jmv.21249
2-s2.0-48249138975
Autor
Da Silva L.H.A.
Spilki F.R.
Riccetto A.G.L.
De Almeida R.S.
Baracat E.C.E.
Arns C.W.
Institución
Resumen
Human respiratory syncytial virus (hRSV) is recognized as the most important viral agent of serious respiratory tract diseases in the pediatric population worldwide. A prospective study for hRSV was conducted in children ageing less than 1 year admitted in two university hospitals in Campinas, São Paulo, Brazil. The aim of the present study was to investigate the genetic variability of both A and B subgroups of hRSV isolated during an epidemic period in the Campinas metropolitan region, Brazil, by sequencing a variable region of the G protein gene. Phylogenetic trees were constructed from alignments of sequences available in the GenBank database and Brazil isolates for hRSV A and B. The data demonstrate that Brazilian isolates clusters together with A and B viruses from Kenya, New Zealand, South Africa, West Virginia, United States (CH, Rochester), and other Brazilian isolates. Phylogenetic analysis of subgroup A isolates showed that the sequences obtained on the present study falls on three clusters, namely GA2, GA5, and SAA1 that co-circulate during the analyzed period. Subgroup B isolates detected belongs to three genotypes, GB3 (SAB3) and BA (BAIII). Different subgroup B genotypes were detected and BA isolates present in our samples showed some degree of genetic variability. This is one of the first reports on the molecular epidemiology of hRSV strains from the Campinas metropolitan region, São Paulo state, Brazil. And is also the first description of the circulation pattern of hRSV genotypes in two university hospitals, revealing interesting differences between the two subgroups of the virus. © 2008 Wiley-Liss, Inc. 80 9 1653 1660 Anderson, L.J., Hierholzer, J.C., Tsou, C., Hendry, R.M., Fernie, B.F., Stone, Y., McIntosh, K., Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies (1985) J Infect Dis, 151, pp. 626-633 Anderson, L.J., Hendry, R.M., Piedik, L.T., Tsou, C., McIntosh, K., Multicenter study of strains of respiratory syncytial virus (1991) J Infect Dis, 163, pp. 687-692 Cane, P.A., Pringle, C.R., Respiratory synctial viral heterogeneity during an epidemic analysis by limited nucleotide sequencing (SH gene) and restriction mapping (N gene) (1991) J Gen Virol, 27, pp. 349-357 Cane, P.A., Matthews, D., Pringle, C., Identification of variable domains of attachment G protein of subgroup A respiratory syncytial viruses (1991) J Gen Virol, 72, pp. 2091-2096 Choi, E.H., Lee, H.J., Genetic diversity and molecular epidemiology of the G protein of subgroups A and B of respiratory syncytial viruses isolated over 9 consecutive epidemics in Korea (2000) J Infect Dis, 181, pp. 1547-1556 Collins, P.L., Chanock, R.M., Murphy, B.R., Respiratory syncytial virus (2001) Fields virology, pp. 1443-1486. , Knipe DM, Howley PM, editors, 4th edition. Philadelphia: Lippincott-Williams and Wilkns. pp Falsey, A.R., Wash, E.E., Respiratory syncytial virus infections in adults (2000) Clin Microb Rev, 13, pp. 371-384 Frabasile, S.A., Facal, D.L., Videla, M., Galiano, M., Sierra, M.J., Ruchansky, D., Vitureira, N., Arbiza, J., Antigenic and genetic variability of human respiratory syncytial viruses (group A) isolated in Uruguay and Argentina: 1993-2001 (2003) J Med Virol, 71, pp. 305-312 Galiano, M., Palomo, C., Videla, C.M., Arbiza, J., Melero, J.A., Carballal, G., Genetic and antigenic variability of human respiratory syncytial virus (groups A and B) isolated over seven consecutive seasons in Argentina (1995 to 2001) (2005) J Clin Microb, 43, pp. 2266-2273 Hall TA. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95-98Joffe, S., Ray, G.T., Escobar, G.J., Black, S.B., Lieu, T.A., Cost-effectiveness of respiratory syncytial virus prophylaxis among preterm infants (1999) Pediatrics, 104, pp. 419-427 Johnson, P.R., Olmsted, R.A., Collins, P.L., The G glycoprotein of human respiratory syncytial viruses of subgroups A and B: Extensive sequence divergence antigenically related proteins (1987) Proc Natl Acad Sci, 84, pp. 5625-5629 Kuroiwa, Y., Nagai, L., Okita, I., Kase, T., Nakayama Tsutsumi, H., A phylogenetic study of human respiratory syncytial viruses group A and B strains isolated in two cities in Japan from 1980-2002 (2005) J Med Virol, 76, pp. 241-247 Levine, S., Klaiber-Franco, R., Paradiso, P.R., Demonstration that glycoprotein G is the attachment protein of respiratory syncytial virus (1987) J Gen Virol, 68, pp. 2521-2524 Marchetti, A., Lau, H., Magar, R., Wang, L., Devercelli, G., Impact of palivizumab on expected costs of respiratory syncytial virus infection in preterm infants: Potential for savings (1999) Clin Ther, 21, pp. 752-766 Matheson, J.W., Rich, F.J., Cohet, C., Grimwood, K., Hung, Q.S., Penny, D., Hendy, M.D., Kirman, J., Distinct patterns of evolution between respiratory syncytial virus subgroups A and B from New Zealand isolates collected over thirty years (2006) J Med Virol, 78, pp. 1354-1364 Melero, J.A., García-Barreno, B., Martínez, I., Pringle, C.R., Cane, P.A., Antigenic structure, evolution, and immunobiology o human respiratory syncytial virus attachment G protein (1997) J Gen Virol, 78, pp. 2411-2418 Mello, W.A., Silva, C., Epidemiological aspects of RSV subgroups in Belém, Brazil (1992) Bol Lat Ame, 1, p. 8 Moura, F.E.A., Blanc, A., Frabasile, S., Delfraro, A., Sierra, M.J., Tome, L., Ramos, A.G., Arbiza, J., Genetic diversity of respiratory syncytial virus isolated during an epidemic period from children of northeastern Brazil (2004) J Med Virol, 74, pp. 156-160 Mufson, M.A., Belshe, R.B., Orvell, C., Norrby, E., Two distinct subtypes of human respiratory syncytial virus (1985) J Gen Virol, 66, pp. 2111-2124 Mufson, M.A., Belshe, R.B., Orvell, C., Norrby, E., Subgroup characteristics of respiratory syncytial virus strains from children with consecutive infections (1987) J Clin Microb, 25, pp. 1535-1539 Nagai, K., Kamasaki, H., Kuroiwa, Y., Okita, L., Tsutsumi, H., Nosocominal outbreak of respiratory virus subgroup B variants with the 60 nucleotides-duplicated G protein gene (2004) J Med Virol, 74, pp. 161-165 Paes, B.A., Current strategies in the prevention of respiratory syncytial virus disease (2003) Paediatr Respir Rev, 4, pp. 21-27 Parveen, S., Sullender, W.M., Fowler, K., Lefkowitz, E.J., Kapoor, S.K., Broor, S., Genetic variability in the G protein gene of group A and B respiratory syncytial virus from India (2000) J Clin Microb, 44, pp. 3055-3064 Parveen, S., Broor, S., Kapoor, S.K., Fowler, K., Sullender, W.M., Genetic diversity among respiratory syncytial viruses that have caused repeated infections in children from rural India (2006) J Med Virol, 78, pp. 659-665 Parveen, S., Sullender, W.M., Fowler, K., Lefkowitz, E.J., Kapoor, S.K., Brorr, S., Genetic variability in the G protein gene of group A and B respiratory syncytial viruses from India (2006) J Clin Microb, 44, pp. 3055-3064 Peret, T.C.T., Hall, C.B., Schanabel, K.C., Golub, J.A., Anderson, L., Circulation patterns of genetically distinct group A and B strains of human respiratory syncytial virus in community (1998) J Gen Virol, 79, pp. 221-2229 Rafiefard, F., Johansson, B., Tecle, T., Örvell, C., Molecular epidemiology of respiratory syncytial virus (RSV) of group A in Stockholm, Sweden, between 1965 and 2003 (2004) Virus Res, 105, pp. 137-145 Riccetto, A.G.L., Ribeiro, J.D., Silva, M.T.N., Almeida, R.S., Arns, C.W., Baracat, E.C.E., Respiratory syncytial vírus (RSV) in infants hospitalized for acute lower respiratory tract disease: Incidence and associated risks (2006) Braz J Infect Dis, 10, pp. 357-361 Rueda, P., Palomo, C., García-Barreno, B., Melero, J.A., The three C-terminal residues of human respiratory syncytial virus G glycoprotein (long strain) are essential for integrity of multiple epitopes distinguishable by antiidiotypic antibodies (1995) Virol Immunol, 8, pp. 37-46 Sanz, M.C., Kew, O.M., Anderson, L.J., Genetic heterogeneity of the attachment glycoprotein G among group A respiratory syncytial viruses (1994) Virus Res, 33, pp. 203-217 Sato, M., Saito, R., Sakai, T., Sano, Y., Nishikawa, M., Sasaki, A., Shobugawa, Y., Suzuki, H., Molecular epidemiology of respiratory syncytial virus infections among children with acute respiratory symptoms in a community over three seasons (2005) J Clin Microb, 43, pp. 36-40 Scott, P.D., Ochola, R., Ngama, M., Okiro, E.A., Nokes, D.J., Medley, G.F., Cane, P., Molecular epidemiology of respiratory syncytial virus in Kilifi district, Kenya (2004) J Med Virol, 74, pp. 344-354 Siqueira, M.M., Nascimento, J.P., Anderson, L.J., Antigenic characterization of respiratory syncytial virus group A and B isolates in Rio de Janeiro, Brazil (1991) J Clin Microb, 29, pp. 557-559 Straliotto, S.M., Roitman, B., Lima, J.B., Fischer, G.B., Siqueira, M.M., Respiratory syncytial virus (RSV) bronquiolits: Comparative study of RSV groups A and B infected children (1994) Rev Soc Bras Med Trop, 27, pp. 1-4 Straliotto, S.M., Nestor, S.M., Siqueira, M.M., Respiratory syncytial virus groups A and B in Porto Alegre, Brazil, from 1990 to 1995 and 1998 (2001) Mem Inst Oswaldo Cruz, 92, pp. 155-158 Sullender, W.M., Respiratory syncytial virus genetic and antigenic diversity (2000) Clin Microb Rev, 13, pp. 1-15 Sullender, W.M., Mufson, M.A., Anderson, L.J., Wertz, G.W., Genetic diversity of the attachment protein of subgroup B respiratory syncytial viruses (1991) J Virol, 65, pp. 5425-5434 Sullender, W.M., Sun, L., Anderson, L.J., Analysis of respiratory syncytial virus genetic variability with amplified cDNAs (1993) J Clin Microb, 31, pp. 1224-1231 Sullender, W.M., Mufson, M.A., Prince, G.A., Anderson, L.J., Wertz, G.W., Antigenic and genetic diversity among the attachment proteins of group A respiratory syncytial viruses that have repeat infections in children (1998) J Infect Dis, 178, pp. 925-932 Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596-1599Thompson, J.D., Higgins, D.G., Gibson, T.J., Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice (1994) Nucleic Acids Res, 22, pp. 4673-4680 Trento, A., Galiano, M., Videla, C., Caballal, G., García-Barreno, B., Melero, J.A., Palomo, C., Mayor changes in the G protein of human respiratory syncytial virus isolates introduced by a duplication of 60 nucleotides (2003) J Gen Virol, 84, pp. 3115-3120 Trento, A., Viegas, M., Galliano, M., Videla, C., Garballal, G., Mistchenko, A.S., Melero, J.A., Natural history of human respiratory syncytial virus inferred from phylogenetic analysis of the attachment (G) glycoprotein with a 60-nucleotide duplication (2006) J Virol, 80, pp. 975-984 Venter, M., Mashi, S.A., Tiemessen, C.T., Schoub, B.D., Genetic diversity and molecular epidemiology of respiratory syncytial virus over four consecutive seasons in South Africa: Identification of new subgroup A and B genotypes (2001) J Gen Virol, 82, pp. 2117-2124 Viegas, M., Mistchenko, A.S., Molecular epidemiology of human respiratory syncytial virus subgroup A over a six-year period (1999-2004) in Argentina (2005) J Med Virol, 77, pp. 302-310 Waris, M., Pattern of respiratory syncytial virus epidemics in Finland: Two-year cycles with alternating prevalence of groups A and B (1991) J Infect Dis, 163, pp. 464-469 Wertz, G.W., Collins, P.L., Huang, Y., Gruber, C., Levine, S., Ball, L.A., Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein (1985) Natl Acad Sci USA, 82, pp. 4075-4079 Zheng, H., Peret, T.C., Randoph, V.B., Cowley, J.C., Anderson, L.J., Strain-Specific Reverse Transcriptase PCR Assay: Means to distinguish candidate vaccine from wild-type strains of respiratory syncytial virus (1996) J Clin Microb, 34, pp. 334-337 Zlateva, K.T., Lemey, P., Moes, E., Vandamme, A.M., Van Ranst, M., Genetic variability and molecular evolution of the human respiratory syncytial virus subgroup B attachment G protein (2005) J Virol, 79, pp. 9157-9167 Zlateva, K.T., Vijgen, L., Dekeersmaeker, N., Naranjo, C., Van Ranst, M., Subgroup prevalence and genotype circulation patterns of human respiratory syncytial virus in Belgium during ten successive epidemic seasons (2007) J Clin Microb, 45, pp. 3022-3030