Artículos de revistas
Optimization Of A Process Of Continuous Enzyme Purification By Surface Response Analysis
Registro en:
Food Control. , v. 4, n. 3, p. 144 - 148, 1993.
9567135
10.1016/0956-7135(93)90301-4
2-s2.0-38249004424
Autor
Rodrigues M.I.
Filho R.M.
Maugeri F.
Institución
Resumen
The methodology considered in this work for the optimization of an enzyme purification process by continuous adsorption recycle extraction (CARE) makes use of surface response analysis. The advantage of such approach is mainly concerned with the fact that it does not require the use of reduced models. In this way, through a detailed model of the system, it is possible to account for operational conditions, the whole set of process variables and design detail in the analysis. With this information, the main effects of each variable (among a group of seven) have been quantified, so that high yield can be achieved handling the enzyme feed concentration, the adsorption stage feed flow rate, the bed porosity and the recycling flow rate. For the productivity, beyond the enzyme feed concentration and the adsorption stage feed flow rate, the volumes of both adsorption and desorption are important. Through a two-star factorial procedure, it was possible to obtain mathematical relationships for the yield and productivity which are very useful in defining operating policy and design details in the search for optimal conditions. © 1993. 4 3 144 148 Box, Hunter, Hunter, (1978) Statistics for Experimenters — an Introduction to Design, Data Analysis and Model Building, , John Wiley & Sons, New York Chase, Prediction of the performance of preparative affinity chromatography (1984) J. Chromatogr., 297, pp. 179-202 Chase, Affinity separations utilising immobilized monoclonal antibodies — a new tool for the biochemical engineer (1984) Chem. Eng. Sci., 39, pp. 1099-1125 Cowan, Goisling, Laws, Sweetenham, Physical and mathematical modelling to aid scale up of liquid chromatography (1986) Journal of Chromatography A, 363, pp. 37-56 Khuri, Cornell, (1987) Response Surface-Design and Analyses, , Marcel Dekker, ASQC Quality Press, New York Pungor, Afeyan, Gordon, Cooney, Continuous affinity — recycle extraction a novel protein separation technique (1987) Bio/Technology, 5, pp. 604-608 Rodrigues, Zaror, Maugeri, Asenjo, Dynamic modelling, simulation and control of continuous adsorption recycle extraction (1992) Chem. Eng. Sci., 47, pp. 263-269