Artículos de revistas
Identification Of A New Vascular Smooth Muscle Contracting Polypeptide In Phoneutria Nigriventer Spider Venom
Registro en:
Biochemical Pharmacology. , v. 46, n. 6, p. 1092 - 1095, 1993.
62952
10.1016/0006-2952(93)90676-N
2-s2.0-0027214207
Autor
Bento A.C.
Novello J.C.
Marangoni S.
Antunes E.
Giglio J.R.
Oliveira B.
^de Nucci G.
Institución
Resumen
The fractionation of Phoneutria nigriventer spider venom by gel filtration (Sephadex G-10-120) followed by ion-exchange chromatography (microgranular CM-cellulose-52) resulted in sixteen fractions (C1 to CXVI) from which CVII+VIII, CIX and CX+XI caused dose-dependent and short-lived contractions of both arterial and venous rabbit vessels. Fraction CX+XI was further purified by a reverse phase HPLC, and a contractile polypeptide (PNV2) was isolated. The amino terminal sequence of PNV2 (LAKRADICQPGKTSQRACET) indicated that it represents a pure polypeptide consisting of a single chain. Furthermore, the amino acid analysis of PNV2 revealed the presence of four disulfide bridges, a high content in Lys (14%), Glx (11%), and the absence of His. The global amino acid composition showed that this polypeptide is composed of 102 residues (Trp not included) with a calculated molecular weight of 12,114. Whether this peptide is responsible for the vascular alterations observed in Phoneutria envenomation, such as lung edema and priapism, remains to be further investigated. © 1993. 46 6 1092 1095 Lucas, Spiders in Brazil (1988) Toxicon, 26, pp. 759-772 Vital-Brazil, Vellard, Contribuição ao estudo do veneno das aranhas I (1925) Mem Inst Butantan, 2, pp. 5-77 Vital-Brazil, Vellard, Contribuição ao estudo do veneno das aranhas II (1926) Mem Inst Butantan, 3, pp. 3-77 Vital-Brazil, Vellard, Contribuição ao estudo do veneno das aranhas III (1926) Mem Inst Butantan, 3, pp. 243-294 Schenberg, Pereira-Lima, Phoneutria nigriventer venom. Pharmacology and biochemistry of its components (1971) Venomous Animals and Their Venoms, 3, pp. 279-297. , W. Bucherl, E.E. Buckley, Academic Press, New York Fontana, Vital Brazil, Mode of action of Phoneutria nigriventer spider venom at the isolated phrenic nerve-diaphragm of the rat (1985) Braz J Med Biol Res, 18, pp. 557-565 Vital-Brazil, Leite, Fontana, Modo de ação da peçonha da aranha armadeira, Phoneutria nigriventer (Keyserling, 1891), nas aurículas isoladas de cobaia (1988) Ciênc Cult, 40, pp. 181-185 Antunes, Marangoni, Brain, de Nucci, Phoneutria nigriventer (armed spider) venom induces increased vascular permeability in rat and rabbit skin in vivo (1992) Toxicon, 30, pp. 1011-1016 Antunes, Marangoni, Borges, Fontana, de Nucci, Pharmacological profile of Phoneutria nigriventer venom on rabbit vascular smooth muscle (1990) British Journal of Pharmacology, 101, p. 508P Antunes, Marangoni, Borges, Hyslop, Fontana, de Nucci, Effects of Phoneutria nigriventer venom on rabbit vascular smooth muscle (1993) Braz J Med Biol Res, 26, pp. 81-91 Marangoni, Borges, Marangoni, Antunes, Vieira, Novello, Domont, de Nucci, Biochemical characterization of a vascular smooth muscle contracting polypeptide purified from Phoneutria nigriventer (armed spider) venom (1993) Toxicon, 31, pp. 377-384 Entwistle, Johnstone, Medzihradszky, May, Isolation of a pure toxic polypeptide from the venom of the spider Phoneutria nigriventer and its neurophysiological activity on an insect femur preparation (1982) Toxicon, 20, pp. 1059-1067 Rezende, Jr., Cordeiro, Oliveira, Diniz, Isolation of neurotoxic peptides from the venom of the armed spider Phoneutria nigriventer (1991) Toxicon, 29, pp. 1225-1233 Heinrikson, Meredith, Amino acid analysis by reverse-phase high-performance liquid chromatography: Precolumn derivatization with phenylisothiocyanate (1984) Anal Biochem, 136, pp. 65-74 Vane, The use of isolated organs for detecting active substances in the circulating blood (1964) Br J Pharmacol Chemother, 23, pp. 360-373 Paton, A pendulum auxotonic lever (1957) J Physiol (Lond), 137, pp. 35P-36P Diniz, Cordeiro, Junior, Kelly, Fischer, Reiman, Oliveira, Richardson, The purification and amino acid sequence of the lethal neurotoxin Tx1 from the venom of the Brazilian “armed” spider Phoneutria nigriventer (1990) FEBS Lett, 263, pp. 251-253