Artículos de revistas
Chemometric Analysis Of The Multidrug Resistance In Strains Of Penicillium Digitatum
Registro en:
Sar And Qsar In Environmental Research. , v. 19, n. 1-2, p. 55 - 70, 2008.
1062936X
10.1080/10629360701844118
2-s2.0-40249086735
Autor
Kiralj R.
Ferreira M.M.C.
Institución
Resumen
Multidrug resistance activities pECr50 of diverse strains of pathogenic fungus Penicillium digitatum against seven toxicants were studied by Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA). Fungal growth data (radii, circumferences, surface areas of fungal colonies, radius differences and ratios) in absence and presence of toxicants were used to derive eight new descriptors for 35 fungal strains. This data set was studied by PCA and HCA, and was correlated with the genome descriptor PCR for expression of gene CYP51 by Partial Least Squares (PLS) regression. Both analyses of pECr50 data and of fungal growth data have identified baseline resistance character, origin and target fruits of the fungal strains. In addition, the analysis of fungal growth data shows that fungal growth morphology is multivariate by nature, meaning that experimental data can be explored more intensely than in usual practice. Fungal growth is directly related to the production of enzyme P45014DM as the main resistance mechanism of P. digitatum against demethylation inhibitors. This is visible from a parsimonious PLS model (two principal components, Q2 = 0.985, R2 = 0.991, SEV = 0.028), validated with eight strains in the external validation set (Q2ev = 0.982, R2ev = 0.990, SECev = 0.025). Chemometric methods in exploring bioassay data are promising approaches to obtain useful information on fungal resistance and to apply these findings in practice. 19 1-2 55 70 Nakaune, R., A novel ATP-binding cassette transporter involved in multidrug resistance in the phytopathogenic fungus Penicillium digitatum (1998) Appl. Environ. Microbiol, 64, pp. 3983-3988 Hamamoto, H., Tandem repeat of a transcriptional enhancer upstream of the sterol 14α-demethylase gene (CYP51) in Penicillium digitatum (2000) Appl. Environ, Microbiol, 66, pp. 3421-3426 Hamamoto, H., PCR-based detection of sterol demethylation inhibitor-resistant strains of Penicillium digitatum (2001) Pest. Manage. Sci, 57, pp. 839-843 Hamamoto, H., The role of the ABC transporter gene PMR1 in demethylation inhibitor resistance in Penicillium digitatum (2001) Pestic. Biochem. Physiol, 70, pp. 19-26 Nakaune, R., A novel ABC transporter gene, PMR5, is involved in multidrug resistance in the phytopathogenic fungus Penicillium digitatum (2002) Mol. Genet. Genomics, 267, pp. 179-185 Y. Matsuzaki et al., PMR3 and PMR4, novel ABC transporter genes in the phytopathogenic fungus Penicillium digitatum, play different roles in multidrug susceptibility and pathogenicity. Submitted (March 2004) to the EMBL/GenBank/DDJ databases. Available with the accession number Q68AM7 in the UniProt database at http://www.pir.uniprot.org/ [last accessed on August 12, 2007]Droby, S., Induction of resistance to Penicillium digitatum in grapefruit by the yeast biocontrol agent Candida oleophila (2002) Phytopathol, 92, pp. 393-399 Holmes, G.J., Eckert, J.W., Sensitivity of Penicillium digitatum and P. italicum to postharvest citrus fungicides in California (1999) Phytopathol, 89, pp. 716-721 (2006) FRAC list of plant pathogenic organisms resistant to disease control agents, FRAC Publications, , http://www.frac.info/publication/anhang/List_of_resistant_plant_pathogens_Dec%202006_web.pdf, Fungicide Resistance Action Committee FRAC, Available at, last accessed on October 18, 2007 Lahlali, R., In vitro effects of water activity, temperature and solutes on the growth rate of P. italicum Wehmer and P. digitatum Sacc (2006) J. Appl. Microbiol, 101, pp. 628-636 Brent, K.J., Hollomon, D.W., Fungicide resistance in crop pathogens: How it can be managed? (2007) FRAC Monograph, 1. , http://www.frac.info/frac/publication/anhang/FRAC_Monol_2007_100dpi.pdf, 2nd ed, Available at, last accessed on October 18, 2007 Georgopoulo, S.G., The problem of fungicide resistance (1969) BioScience, 19, pp. 971-973 (2007) SBI Fungicides, FRAC Publications, , http://www.frac.info/frac/work/work_sbif.htm, Fungicide Resistance Action Committee FRAC, Available at, last accessed on October 18, 2007 (2006) FRAC code list 2: Fungicides sorted by modes of action, FRAC Publications, , http://www.frac.info/frac/publication/anhang/FRAC_Code_list2_2006_web.pdf, Fungicide Resistance Action Committee FRAC, Available at, last accessed on October 18, 2007 (2004) Cases of resistance to benzimidazoles, FRAC Publications, , http://www.frac.info/frac/work/2004-06-11Resistance_tables_Benz.pdf, Fungicide Resistance Action Committee FRAC, Available at, last accessed on October 18, 2007 Greenway, W., Ward, S., Whatley, F.R., Uptake of fuberidazole and thiabendazole by Penicillium digitatum, Cunnighamella echinulata and potato slices (1978) New Phytol, 80, pp. 595-605 Resistance: Penicillium digitatum, , http://e-phy.agricul.ture.gouv.fr/ecoacs/3a1286.htm, Ministére de l'Agriculture et de la Pêche French Ministry of Agriculture and Fishery, Available at, last accessed on August 12, 2007 Ghosoph, J.M., Imazalil resistance linked to a unique insertion sequence in the PdCYP51 promoter region of Penicillium digitatum (2007) Postharvest Biol. Technol, 44, pp. 9-18 Zhu, J., Xie, Q., Li, H., Occurrence of imazalil-resistant biotype of Penicillium digitatum in China and the resistant molecular mechanism (2006) J. Zheijang Univ. Sci. A, 7 (SUPPL.), pp. 362-365 Zhang, J., Timmer, L.W., Preharvest application of fungicides for postharvest disease control on early season tangerine hybrids in Florida (2007) Crop Protect, 26, pp. 886-893 McLeon, K.J., Azole antifungals are potent inhibitors of cytochrome P450 mono-oxygenasese and bacterial growth in mycobacteria and streptomycetes (2002) Microbiology, 148, pp. 2937-2949 Zarn, J.A., Brüschweiler, B.J., Schlatter, J.R., Azole fungicides affect mammalian steroidogenesis by inhibiting sterol 14α-demethylase and aromatase (2003) Environ. Health Perspec, 111, pp. 255-261 Lepesheva, G.I., Waterman, M.R., CYP51 - the omnipotent P450 (2004) Mol. Cell. Endocrinol, 215, pp. 165-170 Saier Jr, M.H., Families of transmembrane transporters selective for amino acids and their derivatives (2000) Microbiology, 146, pp. 1775-1795 Saier Jr, M.H., A functional-phylogenetic classification system for transmembrane solute transporters (2000) Microbiol. Molec. Biol. Rev, 64, pp. 354-411 Jasinski, M., The ATP-binding cassette transporters: Structure, function, and gene family comparison between rice and arabidopsis (2003) Plant Physiol, 131, pp. 1169-1177 Kiralj, R., Ferreira, M.M.C., (2007) Extensive chemometric investigations of the multidrug resistance in strains of the phytopathogenic fungus Penicillium digitatum, , QSAR Comb. Sci, online since July 17 Beebe, K.R., Pell, R., Seasholtz, M.B., (1998) Chemometrics: A practical guide, , Wiley, New York, NY Martens, H., Naes, T., (1989) Multivariate Calibration, , 2nd ed, Wiley, New York, NY Ferreira, M.M.C., Antunes, A.M., Volpe, P.L.O., Quimiometria I: Calibração multivariada, um tutorial (1999) Quim. Nova, 22, pp. 724-731 Ferreira, M.M.C., Multivariate QSAR (2002) J. Braz. Chem. Soc, 13, pp. 742-753 (2001) Matlab 6.1, , Math Works, Inc, Natick, MA (2003) Pirouette 3.11, , Infometrix, Woodinville, WA Kiralj, R., Ferreira, M.M.C., A priori molecular descriptors in QSAR: A case of HIV-I protease inhibitors. II. Molecular graphics and modeling (2003) J. Mol. Graph. Mod, 21, pp. 499-515 Kiralj, R., Takahata, Y., Ferreira, M.M.C., QSAR of progestogens: Use of a priori and computed molecular descriptors and molecular graphics (2003) QSAR Comb. Sci, 22, pp. 430-448 Kiralj, R., Ferreira, M.M.C., Molecular graphics-structural and molecular graphics descriptors in a QSAR study of 17-α- acetoxyprogesterones (2003) J. Braz. Chem. Soc, 14, pp. 20-26 Kiralj, R., Ferreira, M.M.C., Molecular graphics approach to bacterial AcrB protein -ß-lactam antibiotic molecular recognition in drug efflux mechanism (2006) J. Mol. Graph. Mod, 25, pp. 126-145 Wold, S., Eriksson, L., Statistical Validation of QSAR Results (1995) Chemometric Methods in Molecular Design, pp. 309-318. , H. van de Waterbeemd, ed, VCH, Weinheim, Germany Cristner, C., Synthesis and cytotoxic evaluation of cycloheximide derivatives as potential inhibitors of FKBP12 with neuroregenerative properties (1999) J. Med. Chem, 42, pp. 3615-3622 Ferenc, T., Genotoxicity assessment of new synthesized acridine derivatives - 3.6-diamino-10-methyl-9,10-dihydroacridine (1999) Mutation Res, 444, pp. 463-470 Farm, Y.C., Enzymatic and nonenzymatic production of free radicals from the carcinogens 4-nitroquinoline N-oxide and 4- hydroxylaminoquinoline N-oxide (1999) Chem. Res. Toxicol, 12, pp. 450-458 Ferreira, M.M.C., Kiralj, R., QSAR study of ß-lactam antibiotic efflux by the bacterial multidrug resistance pump AcrB (2004) J. Chemometr, 18, pp. 242-252 Lane, R.P., (1994) Morphometric Discrimination of Pest: Experimental Design, in The Identification and Characterization of Pest Organisms, pp. 215-224. , D.L. Hawkesworth, ed, CAB International, Wallingford, UK Peltroche-Llacsahuanga, H., Differentiation between Candida dubliniensis and Candida albicans by fatty acid methyl ester analysis using gas-liquid chromatography (2000) J. Clin. Microbiol, 38, pp. 3696-3704 Federici, C.T., Phylogenetic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis (1998) Theor. Appl. Genet, 96, pp. 812-822 Nicolasi, E., Citrus phytogeny and genetic origin of important, species as investigated by molecular markers (2000) Theor. Appl. Genet, 100, pp. 1155-1166 Russell, R., Solutions to Penicillium taxonomy crucial to mycotoxin research and health (2004) Res. Microbiol, 155, pp. 507-513 Znidarsic, P., Pavko, A., The morphology of filamentous fungi in submerged cultivations as a bioprocess descriptor (2001) Food Technol, Biotechnol, 39, pp. 237-252