Artículos de revistas
Antibothropic Action Of Casearia Sylvestris Sw. (flacourtiaceae) Extracts
Registro en:
Phytotherapy Research. , v. 22, n. 6, p. 784 - 790, 2008.
0951418X
10.1002/ptr.2365
2-s2.0-46849120005
Autor
Cintra-Francischinelli M.
Silva M.G.
Andreo-Filho N.
Gerenutti M.
Cintra A.C.O.
Giglio J.R.
Leite G.B.
Cruz-Hofling M.A.
Rodrigues-Simioni L.
Oshima-Franco Y.
Institución
Resumen
Casearia sylvestris Sw., popularly known in Brazil as 'guaçatonga', has been used as antitumor, antiseptic, antiulcer, local anaesthetic and healer in folk medicine. Snakebite envenomation by Bothrops jararacussu (Bjssu) constitutes a relevant public health hazard capable of inducing serious local damage in victims. This study examined the pharmacological action of apolar and polar C. sylvestris leaf extracts in reverting the neuromuscular blockade and myonecrosis, which is induced by Bjssu venom and its major toxin bothropstoxin-I on the mouse phrenic nerve-diaphragm preparations. The polar methanol extract (ME) was by far the most efficacious. ME not only prevented myonecrosis and abolished the blockade, but also increased ACh release. Such facilitation in neuromuscular transmission was observed with ME alone, but was accentuated in preparations incubated with ME plus venom or toxin. This established synergy opens an interesting point of investigation because the venom or toxin in contact with ME changes from a blocking to a facilitating effect. It is suggested that rutin, known to have potent antioxidant properties, and one of the components present in the ME, could have a role in the observed effects. Since commercial rutin did not reproduce the ME effects, it is likely that a rutin-containing phytocomplex is neutralizing the bothropic envenoming effects. Copyright © 2008 John Wiley & Sons, Ltd. 22 6 784 790 Bjarnason, J.B., Fox, J.W., Hemorrhagic metalloproteinases from snake venoms (1994) Pharmacol Ther, 62, pp. 325-372 Borges, M.H., Soares, A.M., Rodrigues, V.M., Effects of aqueous extract of Casearia sylvestris (Flacourtiaceae) on actions of snake and bee venoms and on activity of phospholipases A 2 (2000) Comp Biochem Physiol B Biochem Mol Biol, 127, pp. 21-30 Borges, M.H., Soares, A.M., Rodrigues, V.M., Neutralization of proteases from Bothrops snake venoms by aqueous extract from Casearia sylvestris (Flacourtiaceae) (2001) Toxicon, 39, pp. 1863-1869 Bülbring, E., Observation on the isolated phrenic nerve diaphragm preparation of the rat (1946) Br J Pharmacol, 1, pp. 38-61 De Oliveira, M., Cavalcante, W.L., Arruda, E.A., Melo, P.A., Dal-Pai Silva, M., Gallaci, M., Antagonism of myotoxic and paralyzing activities of bothropstoxin-l by suramin (2003) Toxicon, 42, pp. 373-379 Girish, K.S., Kemparaju, K., Inhibition of Naja naja venom hyaluronidase by plant-derived bioactive components and polysaccharides (2005) Biochemistry (Moscow), 70, pp. 948-952 Gutierrez, J.M., Romero, M., Díaz, C., Borkow, G., Ovadia, M., Isolation and characterization of a metalloproteinase with weak hemorrhagic activity from the venom of the snake Bothrops asper (terciopelo) (1995) Toxicon, 33, pp. 19-29 Harbone, J.B., (1998) Phytochemical Methods: A Guide to Modern Techniques of Plants Analysis, , 3rd edn, Chapman & Hall: London Havsteen, B., Flavonoids, a class of natural products of high pharmacological potency (1983) Biochem Pharmacol, 32, pp. 1141-1148 Heluany, N.F., Homsi-Brandeburgo, M., Giglio, J.R., Prado-Franceschi, J., Rodrigues-Simioni, L., Effects induced by bothrop-stoxin, a component from Bothrops jararacussu snake venom, on mouse and chick muscle preparations (1992) Toxicon, 30, pp. 1203-1210 Iwanaga, S., Suzuki, T., Enzymes in snake venoms (1979) Snake Venoms. Handbook of Experimental Pharmacology, pp. 61-158. , Lee CY ed, Springer: New York Jin, G.Z., Yamagata, Y., Tomita, K., Structure of rutin pentamethanol (1990) Chem Pharm Bull, 38, pp. 297-300 Khan, A.A., Ashfaq, A., Ali, M.N., (1979) Pharmacognostic Studies of Selected Indigenous Plants of Pakistan, , Pakistan Forest Institute. Spinezer Printers: Peshawar Mahmood A, Ahmad M, Jabeen A, Zafar M, Nadeem S. 2005. Pharmacognostic studies of some indigenous medicinal plants of Pakistan. Available in http://www.siu.edu/∼ebl/leaflets/abid.htm. Access in 06/03/2005Maistro, E.L., Carvalho, J.C., Mantovani, M.S., Evaluation of the genotoxic potential of the Casearia sylvestris extract on HTC and V79 cells by the comet assay (2004) Toxicol In Vitro, 18, pp. 337-342 Markland, F.S., Snake venoms and the hemostatic system (1998) Toxicon, 36, pp. 1749-1800 Matrisian, L.M., The matrix-degrading metalloproteinases (1992) BioEssays, 14, pp. 455-463 Milani Junior, R., Jorge, M.T., de Campos, F.P., Snake bites by the jararacuçu (Bothrops jararacussu): Clinicopatho-logical studies of 29 proven cases in Sao Paulo State, Brazil (1997) Q J Med, 90, pp. 323-334 (2001) Manual de Diagnóstico e Tratamento de Acidentes por Animais Pegonhentos, , Ministério da Saúde do Brasil, 2nd edn. Fundação Nacional da Saúde: Brasília Mors, W.B., Nascimento, M.C., Pereira, B.M., Pereira, N.A., Plant natural products active against snake bite - the molecular approach (2000) Phytochemistry, 55, pp. 627-642 Oshima-Franco, Y., Alves, C.M.V., Andréo Filho, N., Neutralization of the neuromuscular activity of bothropstoxin-l, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (guaçatonga) (2005) J Venom Anim Toxins Trop Dis, 11, pp. 465-478 Oshima-Franco, Y., Hyslop, S., Cintra, A.C.O., Giglio, J.R., Cruz-Höfling, M.A., Rodrigues-Simioni, L., Neutralizing capacity of commercial bothropic antivenom against Bothrops jararacussu venom and bothropstoxin-l (2000) Muscle Nerve, 23, pp. 1832-1839 Oshima-Franco, Y., Leite, G.B., Dal Belo, C.A., The presynaptic activity of bothropstoxin-l, a myotoxin from Bothrops jararacussu snake venom (2004) Basic Clin Pharmacol Toxicol, 95, pp. 175-182 Oshima-Franco, Y., Leite, G.B., Silva, G.H., Neutralization of the pharmacological effects of bothropstoxin-l from Bothrops jararacussu (jararacuçu) venom by crotoxin antiserum and heparin (2001) Toxicon, 39, pp. 1477-1485 Oshima-Franco, Y., Leite, G.B., Valério, A.A., Rabbit antivenom efficacy against myotoxic and neurotoxic activities of Bothrops jararacussu venom and bothropstoxin-l (2002) J Venom Anim Toxins, 8, pp. 226-243 Pessini, A.C., Takao, T.T., Cavalheiro, E.C., A hyaluronidase from Tityus serrulatus scorpion venom: Isolation, characterization and inhibition by flavonoids (2001) Toxicon, 39, pp. 1495-1504 Randazzo-Moura, P., Leite, G.B., Silva, G.H., Study of myotoxicity of bothropstoxin-l (BthTX-l) using manganese (Mn 2+) in mouse phrenic nerve-diaphragm (PND) and extensor digitorum longus (EDL) preparations (2006) Braz J Morphol Sci, 23, pp. 171-184 Rates, S.M.K., Plants as source of drugs (2001) Toxicon, 39, pp. 603-613 Rodrigues-Simioni, L., Borgese, N., Ceccarelli, B., The effects of Bothrops jararacussu venom and its components on frog nerve-muscle preparation (1983) Neuroscience, 10, pp. 475-489 Rodrigues-Simioni, L., Prado-Franceschi, J., Cintra, A.C.O., Giglio, J.R., Jiang, M.S., Fletcher, J.E., No role for enzymatic activity or dantrolene-sensitive Ca 2+ stores in the muscular effects of bothropstoxin, a Lys49 phospholipase A 2 myotoxin (1995) Toxicon, 33, pp. 1479-1489 Rosenfeld, G., Symptomatology, pathology and treatment of snakebites in South America (1971) Venomous Animals and their Venoms, pp. 345-384. , Bucherl W, Buckley E eds, Academic Press: New York Rucavado, A., Escalante, T., Gutiérrez, J.M., Effect of the metalloproteinase inhibitor batimastat in the systemic toxicity induced by Bothrops asper snake venom: Understanding the role of metalloproteinases in envenomation (2004) Toxicon, 43, pp. 417-424 Selistre, H.S., Queiroz, L.S., Cunha, O.A., De Souza, G.E., Giglio, J.R., Isolation and characterization of hemorrhagic, myonecrotic and edema-inducing toxins from Bothrops insularis (jararaca ilhoa) snake venom (1990) Toxicon, 28, pp. 261-273 Simōes CMO, Schenkel EP, Gosmann G, Mello JCP, Mentz LA, Petrovick PR. 2004. Farmacognosia: da Planta ao Medicamento, 5th edn. UFRGS/UFSC: Porto Alegre/FlorianópolisSoares, A.M., Oshima-Franco, Y., Vieira, C.A., Mn 2+ ions reduce the enzymatic and pharmacological activities of bothropstoxin-l, a myotoxic Lys49 phospholipase A 2 homologue from Bothrops jararacussu snake venom (2002) Int J Biochem Cell Biol, 34, pp. 668-677 Yingprasertchai, S., Bunyasrisawt, S., Ratanabanangkoon, K., Hyaluronidase inhibitors (sodium cromoglycate and sodium auro-thiomalate) reduce the local tissue damage and prolong the survival time of mice injected with Naja kaouthia and Calloselasma rhodostoma venoms (2003) Toxicon, 42, pp. 635-646