Artículos de revistas
Production Of Staphylococcal Enterotoxin D In Foods By Low-enterotoxin-producing Staphylococci
Registro en:
International Journal Of Food Microbiology. , v. 14, n. 1, p. 19 - 25, 1991.
1681605
10.1016/0168-1605(91)90033-L
2-s2.0-0026008757
Autor
Pereira J.L.
Salzberg S.P.
Bergdoll M.S.
Institución
Resumen
The goal of this investigation was to determine whether staphylococcal strains producing enterotoxins at nanogram levels per milliliter in laboratory medium, not detectable by gel diffusion methods, could produce sufficient enterotoxin in foods to result in food poisoning. Three low-enterotoxin D (SED)-producing strains were selected for this research because this enterotoxin is produced in smaller amounts than the other enterotoxins. The foods used were cream pie and cooked ham, divided into two portions, sterile and non-sterile. Each portion was inoculated with known concentrations of the staphylococcal strains under study and incubated for 48 h at 25, 30, and 37°C. Samples were taken after 24 and 48 h. Enterotoxin was detectable in both sterilized and unsterilized cream and ham after 24 h at 37°C with an inoculum of 103/g. Some strains produced detectable amounts of enterotoxin in the sterilized foods after 24 h at 30°C and some produced detectable amounts of enterotoxin in the sterilized foods after 24 h at 25°C with inocula of 104/g. It can be concluded that staphylococcal strains producing enterotoxin at ng/ml levels in laboratory medium, not detectable by gel diffusion methods, can produce sufficient enterotoxin (ng/g) in foods to cause poisoning. © 1991. 14 1 19 25 Casman, Bennet, Dorsey, Stone, The microslide gel double diffusion test for the detection and assay of staphylococcal enterotoxins (1969) Health Lab. Sci., 6, pp. 185-197 Evenson, Hinds, Bernstein, Bergdoll, Estimation of human dose of staphylococcal enterotoxin A from a large outbreak of staphylococcal food poisoning involving chocolate milk (1988) Int. J. Food Microbiol., 7, pp. 311-316 Igarashi, Fujikawa, Shinagaki, Bergdoll, Latex agglutination test for staphylococcal toxic shock syndrome toxin-1 (1986) J. Clin. Microbiol., 23, pp. 509-512 Kokan, Bergdoll, Detection of low-enterotoxin-producing Staphylococcus aureus (1987) Appl. Environ. Microbiol., 53, pp. 2675-2676 Robbins, Gould, Bergdoll, Detecting the enterotoxigenicity of Staphylococcus aureus strains (1974) Appl. Microbiol., 28, pp. 496-950 Wieneke, Enterotoxin production by strains of Staphylococcus aureus isolated from foods and human beings (1974) Journal of Hygiene, 73, pp. 155-162