Artículos de revistas
D2-40, A Novel Lymphatic Endothelial Marker: Identification Of Lymphovascular Invasion And Relationship With Axillary Metastases In Breast Cancer
Registro en:
Jornal Brasileiro De Patologia E Medicina Laboratorial. , v. 44, n. 1, p. 45 - 50, 2008.
16762444
2-s2.0-45349089184
Autor
Marinho V.F.Z.
Sanches F.S.F.
Rocha G.F.S.
Metze K.
Gobbi H.
Institución
Resumen
Background: Studies of lymphatic vessels were limited by the lack of specific markers. Recently, they have become possible due to the release of new D2-40 antibody, a selective marker for lymphatic endothelium. The aim of our study was to compare neoplastic invasion in lymphatic and blood vessels detected in hematoxylin and eosin (H&E) and immunohistochemistry-stained sections. Methodology: A total of 123 cases of invasive mammary carcinomas were studied and sorted out into three subgroups according to axillary staging (macrometastasis, micrometastasis and lymph node negative). Lymphatic vessel invasion (LVI) and blood vessel invasion (BVI) were initially evaluated in histological H&E and immunohistochemistry-stained sequential sections. Lymphatic and blood vessel invasions were assessed by immunohistochemistry, employing D2-40 and CD31 antibodies, respectively. LVI and BVI were related to size, type, histologic grade of primary tumors, and the presence of metastasis. Results: LVI was detected through H&E staining procedure in 17/123 cases (13.8%), and through immunohistochemistry procedure in 35/123 cases (28.5%) (kappa = 0.433). BVI was detected through H&E in 5/123 cases 4.1%), and through immunohistochemistry in 19/123 cases (15.4%) (kappa = 0.198). LVI and BVI were positively related to higher histologic grade of primary tumors (p < 0.05). LVI was also positively related to the presence of macrometastasis. Conclusion: The detection of lymphatic and blood vessel invasions through immunohistochemistry employing D2-40 and CD31 was higher than the detection through H&E, and it was related to higher tumor grade and metastasis in axillary lymph nodes. 44 1 45 50 ACS, G., Extensive retraction artifact correlates with lymphatic invasion and nodal metastasis and predicts poor outcome in early stage breast carcinoma (2007) Am J Surg Pathol, 31 (1), pp. 129-140 AGARWAL, B., Lymphangiogenesis does not occur in breast cancer (2005) Am J Surg Pathol, 29 (11), pp. 1449-1455 AOKI, Y., TOSATO, G., Lymphatic regeneration: New insights from VEGFR-3 blockade (2005) J Natl Cancer Inst, 97 (1), pp. 2-3 ARNAOUT-ALKARAIN, A., Significance of lymph vessel invasion identified by the endothelial lymphatic marker D2-40 in node negative breast cancer (2007) Mod Pathol, 20 (2), pp. 183-191 CHOI, W.W., Angiogenic and lymphangiogenic microvessel density in breast carcinoma: Correlation with clinicopathologic parameters and VEGF-family gene expression (2005) Mod Pathol, 18 (1), pp. 143-152 CLARIJS, R., RUITER, D.J., de WAAL, R.M., Lymphangiogenesis in malignant tumours: Does it occur? (2001) J Pathol, 193 (2), pp. 143-146 CUNNICK, G.H., Lymphangiogenesis and breast cancer metastasis (2002) Histol Histopathol, 17 (3), pp. 863-870 ELSTON, C.W., ELLIS, I.O., Pathological prognostic factors in breast cancer. I. The value of histological grade in breast cancer: Experience from a large study with long-term follow-up (1991) Histopathology, 19 (5), pp. 403-410 EVANGELOU, E., KYZAS, P.A., TRIKALINOS, T.A., Comparison of the diagnostic accuracy of lymphatic endothelial markers: Bayesian approach (2005) Mod Pathol, 18 (11), pp. 1490-1497 FITZGIBBONS, P.L., Prognostic factors in breast cancer. College of American Pathologists Consensus Statement 1999 (2000) Arch Pathol Lab Med, 124 (7), pp. 966-978 GIORGADZE, T.A., Lymphatic vessel density is significantly increased in melanoma (2004) J Cutan Pathol, 31 (10), pp. 672-677 GREENE, F. L. et al. Breast. In: AJCC Cancer Staging Manual. 6th ed. New York: Springer, 2002. Cap. 25 p. 223-40KAHN, H.J., MARKS, A., A new monoclonal antibody, D2-40, for detection of lymphatic invasion in primary tumors (2002) Lab Invest, 82 (9), pp. 1255-1257 KAHN, H.J., BAILEY, D., MARKS, A., Monoclonal antibody D2-40, a new marker of lymphatic endothelium, reacts with Kaposi's sarcoma and a subset of angiosarcomas (2002) Mod Pathol, 15 (4), pp. 434-440 NIAKOSARI, F., Detection of lymphatic invasion in primary melanoma with monoclonal antibody D2-40: A new selective immunohistochemical marker of lymphatic endothelium (2005) Arch Dermatol, 141 (4), pp. 440-444 NISATO, R.E., TILLE, J.C., PEPPER, M.S., Lymphangiogenesis and tumor metastasis (2003) Thromb Haemost, 90 (4), pp. 591-597 PAGE, D.L., JENSEN, R.A., SIMPSON, J.F., Routinely available indicators of prognosis in breast cancer (1998) Breast Cancer Res Treat, 51 (3), pp. 195-208 PEPPER, M.S., Lymphangiogenesis and tumor metastasis: Myth or reality? (2001) Clin Cancer Res, 7 (3), pp. 462-468 SCHOPPMANN, S.F., Prognostic value of lymphangiogenesis and lymphovascular invasion in invasive breast cancer (2004) Ann Surg, 240 (2), pp. 306-312 van den, EYNDEN, G.G., Distinguishing blood and lymph vessel invasion in breast cancer: A prospective immunohistochemical study (2006) Br J Cancer, 94 (11), pp. 1643-1649 van der, AUWERA, I., Tumor lymphangiogenesis in inflammatory breast carcinoma: A histomorphometric study (2005) Clin Cancer Res, 11 (21), pp. 7637-7642 VLEUGEL, M.M., Lack of lymphangiogenesis during breast carcinogenesis (2004) J Clin Pathol, 57 (7), pp. 746-751 YONEMURA, Y., Evaluation of lymphatic invasion in primary gastric cancer by a new monoclonal antibody, D2-40 (2006) Hum Pathol, 37 (9), pp. 1193-1199