Artículos de revistas
Patch-clamp Experiments At Isolated Cells And In Intact Tissue Of The Chicken Retina Correlated To Potassium Channels And The Retinal Spreading Depression
Registro en:
Electrochimica Acta. , v. 42, n. 20-22, p. 3231 - 3240, 1997.
134686
2-s2.0-0030693473
Autor
Hanke W.
Mezler M.
Brand S.
Ladewig T.
Goldermann M.
Fernandes De Lima V.M.
Institución
Resumen
Patch-clamp experiments are well suited to investigate membrane transport processes on the molecular level. Such experiments can be performed on isolated cells, fragments of cells, and cells in tissue of different degrees of integration, under physiological or pathological conditions. We have used the chicken retina, which is a true piece of central grey matter without any blood-vessels to investigate the behaviour of ion channels, especially potassium channels, related to the retinal spreading depression (SD). The potassium channels of neurons mainly were of shorter lifetime and lower open state probability than those of glia (Müller) cells, and also conductances and selectivities were different. Both types of cells have voltage-sensitive as well as not voltage sensitive potassium channels. The properties of an excitation-depression wave in neuronal tissue (SD) can be explained at least partially on a molecular level by our results. In addition to the electrophysiological experiments, the whole-cell configuration of the patch-clamp technique has been used to fill cells with fluorescent dyes (especially Lucifer-Yellow), thereby identifying the cell under investigation. © 1997 Elsevier Science Ltd. 42 20-22 3231 3240 Leao, A.A.P., (1944) J. Neurophysiol., 7, p. 359 Bures, J., Buresova, O., Krivanek, J., (1974) The Mechanism and Application of Leao's Spreading Depression of Electroencephalographic Activity, , Academic Press, New York Milner, P.M., (1958) Electroenceph. Clin. Neurophysiol., 10, p. 705 Martins-Ferreira, H., Olivera, C.G., (1966) J. Neurophysiol., 29, p. 715 Wiedermann, M., Fernandes De Lima, V.M., Hanke, W., (1996) Naunyn Schmiedbergs Arch. Pharmacol., 353, p. 552 Hamil, O.P., Marty, E., Neher, E., Sakmann, B., Sigworth, F.J., (1981) Pflügers Arch. Europ. J. Physiol., 391, p. 85 Tuckwell, H.C., Miura, R.M., (1978) Biophys. J., 23, p. 257 Grafenstein, B., (1956) J. Neurophysiol., 19, p. 154 Fernandes De Lima, V.M., Scheller, D., Tegtmeier, F., Hanke, W., Schlue, W.-R., (1993) Brain Res., 641, p. 176 Sakmann, B., Neher, E., (1995) Single Channel Recording, , Plenum Press, New York Fernandes De Lima, V.M., Goldermann, M., Hanke, W., (1997) Spreading Depression: A Retinal Model. Biological Techniques Series, , Academic Press, in press Dempster, J., (1993) Computer Analysis of Electrophysiological Signals, , Academic Press, London Stuart, W.W., (1981) Nature, 292, p. 17 Hanke, W., Fernandes De Lima, V.M., Schlue, W.-R., (1993) Migraine: Basic Mechanisms and Treatment, p. 573. , (Edited by Lehmenkühler, A, K-H. Grotemeyer, F. Tegtmeier), Urban Schwarzenberg, Milchen Dowling, J., (1987) The Retina: An Approachable Part of the Brain, , Harvard University Press, Cambridge U.S.A Needergard, M., Cooper, A.J.L., Goldmann, S.A., (1995) J. Neurobiol., 28, p. 433 Brand, S., Hanke, W., (1996) J. Brain Res., 37, p. 73 Martins-Ferreira, H., Ribeiro, L.J.C., (1995) Braz. J. Med. Biol. Res., 28, p. 991 Lauritzen, M., (1992) Path. Biol., 4, p. 332