Artículos de revistas
Purification And Preliminary Crystallographic Analysis Of A New Lys49-pla2 From B. Jararacussu
Registro en:
International Journal Of Molecular Sciences. , v. 9, n. 5, p. 736 - 750, 2008.
14220067
10.3390/ijms9050736
2-s2.0-44649177716
Autor
Dos Santos M.L.
Fagundes F.H.R.
Teixeira B.R.F.
Toyama M.H.
Aparicio R.
Institución
Resumen
BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the asymmetric unit (ASU). Synchrotron radiation diffraction data were collected to 2.0 Å resolution and 1.9 Å resolution for crystals belonging to the space group P2 12 12 1 (a = 48.4 Å, b = 65.3 Å, c = 84.3 Å) and space group P3 121 (a = b = 55.7 Å, c = 127.9 Å), respectively. Refinement is currently in progress and the refined structures are expected to shed light on the unusual platelet aggregation activity observed for BjVIII. © 2008 by the authors; licensee Molecular Diversity Preservation International. 9 5 736 750 van Deenen, L., de Haas, G., Heemskerk, C.H., Hydrolysis of synthetic mixed-acid phosphatides by phospholipase A from human pancreas (1963) Biochim Biophys Acta, 67, pp. 295-304 Higuchi, D.A., Barbosa, C.M.V., Bincoletto, C., Chagas, J.R., Magalhaes, A., Richardson, M., Sanchez, E.F., Pesquero, J., L.. Purification and partial characterization of two phospholipases A2 from Bothrops leucurus (white-tailed-jararaca) snake venom (2007) Biochimie, 89, pp. 319-328 Maraganore, J.M., Merutka, G., Cho, W., Welches, W., Kézdy, F.J., Heinrikson, R.L., A new class of phospholipases A2 with lysine in place of aspartate 49. Functional consequences for calcium and substrate binding (1984) J Biol Chem, 259, pp. 13839-13843 Nakai, M., Nakashima, K.I., Ogawa, T., Shimohigashi, Y., Hattori, S., Chang, C.C., Ohno, M., Purification and primary structure of a myotoxic lysine-49 phospholipase A2 with low lipolytic activity from Trimeresurus gramineus venom (1995) Toxicon, 33, pp. 1469-1478 Koh, D.C.I., Armugam, A., Jeyaseelan, K., Snake venom components and their applications in biomedicine (2006) Cell Mol Life Sci, 63, pp. 3030-3041 Andrião-Escarso, S.H., Soares, A.M., Fontes, M.R.M., Fuly, A.L., Corrêa, F.M.A., Rosa, J.C., Greene, L.J., Giglio, J., R.. Structural and functional characterization of an acidic platelet aggregation inhibitor and hypotensive phospholipase A(2) from Bothrops jararacussu snake venom (2002) Biochem Pharmacol, 64, pp. 723-732 Fuly, A.L., Soares, A.M., Marcussi, S., Giglio, J.R., Guimarães, J.A., Signal transduction pathways involved in the platelet aggregation induced by a D-49 phospholipase A2 isolated from Bothrops jararacussu snake venom (2004) Biochimie, 86, pp. 731-739 Magro, A.J., Murakami, M.T., Marcussi, S., Soares, A.M., Arni, R.K., Fontes, M.R.M., Crystal structure of an acidic platelet aggregation inhibitor and hypotensive phospholipase A2 in the monomeric and dimeric states: Insights into its oligomeric state (2004) Biochem Biophys Res Commun, 323, pp. 24-31 Toyama, M.H., Carneiro, E.M., Marangoni, S., Barbosa, R.L., Corso, G., Boschero, A.C., Biochemical characterization of two crotamine isoforms isolated by a single step RP-HPLC from Crotalus durissus terrificus (South American rattlesnake) venom and their action on insulin secretion by pancreatic islets (2000) Biochim Biophys Acta, 1474, pp. 56-60 Fonseca, F.V., Antunes, E., Morganti, R.P., Monteiro, H.S.A., Martins, A.M.C., Toyama, D.O., Marangoni, S., Toyama, M., H.. Characterization of a new platelet aggregating factor from crotoxin Crotalus durissus cascavella venom (2006) Protein J, 25, pp. 183-192 Schägger, H., von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa (1987) Anal Biochem, 166, pp. 368-379 McPherson, A., Current approaches to macromolecular crystallization (1990) Eur J Biochem, 189, pp. 1-23 Polikarpov, I., Oliva, G., Castellano, E.E., Garratt, R.C., Arruda, P., Leite, A., Craievich, A., The protein crystallography beamline at LNLS, the Brazilian National Synchrotron Light Source (1998) Nucl Instrum Methods Phys Res A, 405, pp. 159-164 Polikarpov, I., Perles, L.A., de Oliveira, R.T., Oliva, G., Castellano, E.E., Garratt, R.C., Craievich, A.. Set-up and Experimental Parameters of the Protein Crystallography Beamline at the Brazilian National Synchrotron Laboratory (1998) J Synchrotron Radiat, 5, pp. 72-76 Leslie, A.G., W.. The integration of macromolecular diffraction data (2006) Acta Crystallogr D Biol Crystallogr, 62, pp. 48-57 Evans, P., Scaling and assessment of data quality (2006) Acta Crystallogr D Biol Crystallogr, 62, pp. 72-82 Navaza, J., Implementation of molecular replacement in AMoRe (2001) Acta Crystallogr D Biol Crystallogr, 57, pp. 1367-1372 Kozin, M.B., Svergun, D.I., Automated matching of high- and low-resolution structural models (2001) J Appl Crystallogr, 34, pp. 33-41 CCP, Collaborative Computational Project, Number 4. The CCP4 suite: Programs for protein crystallography (1994) Acta Crystallogr D Biol Crystallogr, 50, pp. 760-763. , 4 Winn, M., Dodson, E.J., Ralph, A., Collaborative computational project, number 4: Providing programs for protein crystallography (1997) Methods Enzymol, 277, pp. 620-633 Spencer, P.J., Aird, S.D., Boni-Mitake, M., Nascimento, N., Rogero, J.R., A single-step purification of bothropstoxin-1 (1998) Braz J Med Biol Res, 31, pp. 1125-1127 Soares, A.M., Guerra-Sá, R., Borja-Oliveira, C.R., Rodrigues, V.M., Rodrigues-Simioni, L., Rodrigues, V., Fontes, M.R.M., Giglio, J.R., Structural and functional characterization of BnSP-7, a Lys49 myotoxic phospholipase A2 homologue from Bothrops neuwiedi pauloensis venom (2000) Arch Biochem Biophys, 378, pp. 201-209 Arni, R.K., Ward, R.J., Gutiérrez, J.M., Tulinsky, A., Structure of a calcium-independent phospholipase-like myotoxic protein from Bothrops asper venom (1995) Acta Crystallogr D Biol Crystallogr, 51, pp. 311-317 Murakami, M.T., Viçoti, M.M., Abrego, J.R.B., Lourenzoni, M.R., Cintra, A.C.O., Arruda, E.Z., Tomaz, M.A., Arni, R., K.. Interfacial surface charge and free accessibility to the PLA2-active site-like region are essential requirements for the activity of Lys49 PLA2 homologues (2007) Toxicon, 49, pp. 378-387 Toyama, M.H., Soares, A.M., Vieira, C.A., Novello, J.C., Oliveira, B., Giglio, J.R., Marangoni, S.. Amino acid sequence of piratoxin-I, a myotoxin from Bothrops pirajai snake venom, and its biological activity after alkylation with p-bromophenacyl bromide (1998) J Protein Chem, 17, pp. 713-718 Barbosa, P. S. F. Martins, A. M. C. Alves, R. S. Amora, D. N. Martins, R. D. Toyama, M. H. Havt, A. Nascimento, N. R. F. Rocha, V. L. C. Menezes, D. B. Fonteles, M. C. Monteiro, H. S. A. . The role of indomethacin and tezosentan on renal effects induced by Bothrops moojeni Lys49 myotoxin I. Toxicon 2006, 47, 831-837Kramer, R.M., Roberts, E.F., Manetta, J.V., Hyslop, P.A., Jakubowski, J., A.. Thrombin-induced phosphorylation and activation of Ca(2+)-sensitive cytosolic phospholipase A2 in human platelets (1993) J Biol Chem, 268, pp. 26796-26804 Puri, R., N.. Phospholipase A2: Its role in ADP- and thrombin-induced platelet activation mechanisms (1998) Int J Biochem Cell Biol, 30, pp. 1107-1122 Soares, A.M., Andrião-Escarso, S.H., Angulo, Y., Lomonte, B., Gutiérrez, J.M., Marangoni, S., Toyama, M.H., Giglio, J.R., Structural and functional characterization of myotoxin I, a Lys49 phospholipase A(2) homologue from Bothrops moojeni (Caissaca) snake venom (2000) Arch Biochem Biophys, 373, pp. 7-15 Singh, N., Jabeen, T., Pal, A., Sharma, S., Perbandt, M., Betzel, C., Singh, T.P., Crystal structures of the complexes of a group IIA phospholipase A2 with two natural anti-inflammatory agents, anisic acid, and atropine reveal a similar mode of binding (2006) Proteins, 64, pp. 89-100 Kini, R.M., Evans, H.J., A model to explain the pharmacological effects of snake venom phospholipases A2 (1989) Toxicon, 27, pp. 613-635 Gutiérrez, J.M., Lomonte, B., Phospholipase A2 myotoxins from Bothrops snake venoms (1995) Toxicon, 33, pp. 1405-1424 Ownby, C.L., de Araujo, H.S.S., White, S.P., Fletcher, J., E.. Lysine 49 phospholipase A2 proteins (1999) Toxicon, 37, pp. 411-445 Yang, C. C.. In Venom Phospholipase A2 enzymes: Structure, Function and Mechanism Kini, R. M., Ed. John Wiley & Sons: UK, 1997 Chapter Chemical modification and functional sites of phospholipases A2, p 185Stiles, B. G. Choumet, V.. In Venom Phospholipase A2 enzymes: Structure, Function and Mechanism Kini, R. M., Ed. John Wiley & Sons: UK, 1997 Chapter Antibodies studies with venom phospholipases A2, p 223Matthews, B., W.. Solvent content of protein crystals (1968) J Mol Biol, 33, pp. 491-497 Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D., J.. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs (1997) Nucleic Acids Res, 25, pp. 3389-3402 Magro, A.J., Soares, A.M., Giglio, J.R., Fontes, M.R.M., Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A(2): Quaternary structure and inhibition mechanism insights (2003) Biochem Biophys Res Commun, 311, pp. 713-720