Artículos de revistas
Bio-safety Technology In Production Of Bovine Herpesvirus Type 5 (bohv-5) Using An Alternative Serum-free Medium
Registro en:
American Journal Of Biochemistry And Biotechnology. , v. 3, n. 3, p. 125 - 130, 2007.
15533468
2-s2.0-34249683333
Autor
Cardoso T.C.
Ferrari H.F.
Luvizotto M.C.R.
Arns C.W.
Institución
Resumen
The detection and growth of bovine herpes virus type 5 (BoHV-5) was evaluated in three different media without foetal bovine serum or animal protein by infecting three different cell lines. The OPTI-PRO, VP-SFM and RPMI 1640 media were supplemented by L-glutamine, antibiotics added by 5μg of insulin-like growth factor (IGF-I) and used to adapt CER, MDBK and CRIB cells in a statistic continuous culture system. The results obtained by CRIB and MDBK cells adaptation showed steady growth after 10th passages in OPTI-PRO medium, 20th passages in VP-SFM medium and 30th passages in RPMI 1640 medium, respectively. The OPTI-PRO and RPMI 1640 media were not able to support CER growth, even supplemented by IGF-1 and present apoptotic cells at 72h post-infection. The CER cells seeded after adaptation by 10 th passages in VP-SFM medium added by 5μg/20ml of IGF-1 growth factor revealed the best virus titres compared to MDBK and CRIB cell lines. It was concluded that CER, MDBK and CRIB cells infected by BoHV-5 serotype could be used for laboratory diagnosis propagated on much safer culture system of high biotechnology advances. © 2007 Science Publications. 3 3 125 130 Delhon, G., Moraes, M.P., Lu, Z., Afonso, C.L., Flores, E.F., Weiblen, R., Kutish, G.F., Rock, D.L., Genome of Bovine Herpesvirus 5 (2003) J. Virol, 77, pp. 10339-10347 Diel, D.G., S.R. Almeida, M.C.S. Brum, R. Dezengrini, R. Wieben and E.F. Flores, 2007. Acute and latent infection by bovine herpesvirus type 5 in experimentally infected goats. Vet. Microbiol., doi:10.1016/j.vetmic.2006.12. 019Silva, A.D., Spilki, F.R., Franco, A.C., Esteves, P.A., Hübner, S.O., Driemeier, D., Oliveira, A.P., Roehe, P.M., Vaccination with a gE-negative bovine herpesvirus type 1 vaccine confers insufficient protection to a bovine herpesvirus type 5 (2006) Vaccine, 24, pp. 3313-3320 Del Médico Zajac, M.P., M. Puntel, P.I. Zamorano, A.M. Sadir and S.A. Romera, B., 2006. BHV-1 vaccine induces cross-protection against BHV-5 disease in cattle. Res. Vet. Sci., 81:327-334Spilki, F.R., Almeida, R.S., Campalans, J., Arns, C.W., Susceptibility of different cell lines to infection with bovine respiratory syncytial virus (2006) J. Virol. Meth, 131, pp. 130-133 Flores, E.F., Donis, R.O., Isolation of a mutant MDBK cell line resistant to bovine viral diarrhea virus infection due to a block in viral entry (1995) Virology, 208, pp. 565-575 Merten, O.W., (2002) Development of serum-free media for cell growth production of viruses/viral vaccines-Safety issues of animal productus used in serum-free media, 111, pp. 233-257. , Hendriksen C, D. Sesardic, K. Cussler, Editors. Advancing science and elimination of the use of laboratory animals for development and control of vaccines and hormones. Dev. Biol. Basel, Karger Makoschey, B., Patel, J.R., van Gelder, P.T.J.A., Serum-free produced bovine herpesvirus type -1 and bovine parainfluenza type 3 virus vaccines are efficacious and safe (2002) Cytotechnology, 39, pp. 139-145 Butler, M., Burgener, A., Patrick, M., Berry, M., Moffatt, D., Huzel, N., Barnabé, N., Coombs, K., Application of a serum free medium for growth of vero cells and production of Reovirus (2000) Biotech. Prog, 16, pp. 854-858 Cardoso, T.C., Teixeira, M.C.B., Fachin, N., Camargo, T.L., Gomes, D.E., Junior, A.C.O., Anselmo, B., Evaluation of serum and animal free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line (2005) ALTEX, 3, pp. 152-156 Cardoso, T.C., Queiroz-Silva, L.H., Silva, S.E., Albas, A., Pardo, P.E., Tanaka, A.H., Cossy, L.B., Perri, S.H.V., Chicken related (CER) cell line for quantification of rabies neutralizing antibody by fluorescent focus inhibition test (2006) Biologicals, 34, pp. 29-32 Mochizuki, M., Growth characteristics of canine pathogenic viruses in MDCK cells cultured in RPMI 1640 medium without animal protein (2006) Vaccine, 24, pp. 1744-1748 Sunstrom, N.A.S., Gay, R.D., Wong, D.C., Kitchen, N.A., DeBoer, L., Gray, P.P., Insulin-Like Growth Factor-I and transferring mediate growth and survival of Chinese hamster ovary cells (2000) Biotech. Prog, 16, pp. 698-702 Frazzati-Gallina, N.M., Paoli, R.L., Mourão-Funches, R.M., Jorge, S.A.C., Pereira, C.A., Higher production of rabies virus in serum-free medium cell cultures on microcarriers (2001) J. Biotech, 92, pp. 67-72 Murata, T., Takashima, Y., Xuan, X., Otsuka, Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells (1999) Virus Res, 61, pp. 29-41 Genzel, Y., Ritter, J.B., König, S., Alt, R., Reichl, U., Substitution of glutamine by pyruvate to reduce ammonia formation and growth inhibition of mammalian cells (2005) Biotech. Prog, 21, pp. 58-69