Artículos de revistas
Epithelial-stromal Transition Of Mmp-7 Immunolocalization In The Rat Ventral Prostate Following Bilateral Orchiectomy
Registro en:
Cell Biology International. , v. 31, n. 10, p. 1173 - 1178, 2007.
10656995
10.1016/j.cellbi.2007.03.034
2-s2.0-34447109265
Autor
Felisbino S.L.
Justulin Junior L.A.
Carvalho H.F.
Taboga S.R.
Institución
Resumen
Epithelial cells from involuting rat ventral prostate (VP) express Matrilysin (MMP-7) mRNA. Herein, we investigated by immunohistochemistry the MMP-7 protein location and its association with tissue changes following castration in the VP. Normal and castrated adult male Wistar rats were sacrificed at different times after surgery. VP was examined by immunocytochemistry and immunoprecipitation. Castration promoted a shrinking of prostate ducts with an extensive stromal remodeling. In the VP from normal rats, MMP-7 immunoreactivity was found in epithelial secretory granules. Three days after castration, immunostaining for MMP-7 was found in both the epithelial secretory granules and in the stroma just below the epithelium, mainly at the distal ductal tips. At seven and 21 days after castration, the immunostaining for MMP-7 was found only in the stromal space. Immunoprecipitation confirmed the specificity of the primary antibody by rescuing a pro-enzyme form (28 kDa) in the prostate extracts. 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