Artículos de revistas
Mesenchymal Stem Cells Engrafted In A Fibrin Scaffold Stimulate Schwann Cell Reactivity And Axonal Regeneration Following Sciatic Nerve Tubulization
Registro en:
Brain Research Bulletin. Elsevier Inc., v. 112, n. , p. 14 - 24, 2015.
3619230
10.1016/j.brainresbull.2015.01.005
2-s2.0-84921992336
Autor
Cartarozzi L.P.
Spejo A.B.
Ferreira R.S.
Barraviera B.
Duek E.
Carvalho J.L.
Goes A.M.
Oliveira A.L.R.
Institución
Resumen
The present study investigated the effectiveness of mesenchymal stem cells (MSCs) associated with a fibrin scaffold (FS) for the peripheral regenerative process after nerve tubulization. Adult female Lewis rats received a unilateral sciatic nerve transection followed by repair with a polycaprolactone (PCL)-based tubular prosthesis. Sixty days after injury, the regenerated nerves were studied by immunohistochemistry. Anti-p75NTR immunostaining was used to investigate the reactivity of the MSCs. Basal labeling, which was upregulated during the regenerative process, was detected in uninjured nerves and was significantly greater in the MSC-treated group. The presence of GFP-positive MSCs was detected in the nerves, indicating the long term survival of such cells. Moreover, there was co-localization between MSCs and BNDF immunoreactivity, showing a possible mechanism by which MSCs improve the reactivity of SCs. Myelinated axon counting and morphometric analyses showed that MSC engrafting led to a higher degree of fiber compaction combined with a trend of increased myelin sheath thickness, when compared with other groups. The functional result of MSC engrafting was that the animals showed higher motor function recovery at the seventh and eighth week after lesion. The findings herein show that MSC. +. FS therapy improves the nerve regeneration process by positively modulating the reactivity of SCs. 112
14 24 Bain, J.R., Mackinnon, S.E., Hunter, D.A., Functional evaluation of complete sciatic, peroneal and posterior tibial nerve lesions in the rat (1989) Plast. Reconstr. Surg., 83 (1), pp. 129-138 Barbizan, R., Castro, M.V., Rodrigues, A.C., Barraviera, B., Ferreira, R.S., Oliveira, A.L.R., Motor recovery and synaptic preservation after ventral root avulsion and repair with a fibrin sealant derived from snake venom (2013) PLOS ONE, 8 (5), p. e63260 Barker, R.A., Widner, H., Immune problems in central nervous system cell therapy (2004) NeuroRx, 1 (4), pp. 472-481 Barros, L.C., Ferreira, R.S., Barraviera, S.R.C.S., Stolf, H.O., Thomazini-Santos, I.A., Mendes-Giannini, M.J.S., Toscano, E., Barraviera, B., A new fibrin sealant from Crotalus durissus terrificus venom: applications in medicine (2009) J. Toxicol. Environ. Health B, 12 (8), pp. 553-571 Barros, L.C., Soares, A.M., Costa, F.L., Rodrigues, V.M., Fuly, A.L., Giglio, J.R., Gallacci, M., Ferreira, R.S., Biochemical and biological evaluation of gyroxin isolated from Crotalus durissus terrificus venom (2011) J. Venom Anim. Toxins Incl. Trop. Dis., 17 (1), pp. 23-33 Bloch, J., Fine, E.G., Bouche, N., Zurn, A.D., Aebischer, P., Nerve growth factor- and neurotrophin-3-releasing guidance channels promote regeneration of the transected rat dorsal root (2001) Exp. Neurol., 172 (2), pp. 425-432 Bozkurt, A., Scheffel, J., Brook, G.A., Joosten, E.A., Suschek, C.V., O'Dey, D.M., Aspects of static and dynamic motor function in peripheral nerve regeneration: SSI and CatWalk gait analysis (2011) Behav. Brain Res., 219, pp. 55-62 Caplan, A.I., Adult mesenchymal stem cells for tissue engineering versus regenerative medicine (2007) J. Cell. Physiol., 213, pp. 341-347 Carlson, K.B., Singh, P., Feaster, M.M., Ramnarain, A., Pavlides, C., Chen, Z.L., Yu, W.M., Stricklan, S., Mesenchymal stem cells facilitate axon sorting, myelinization and functional recovery in paralyzed mice deficient in Schwann cell-derived laminin (2011) Glia, 59, pp. 267-277 Chen, Y.S., Hsieh, C.L., Tsai, C.C., Chen, T.H., Cheng, W.C., Hu, C.L., Yao, C.H., Peripheral nerve regeneration using silicone rubber chambers filled with collagen, laminin and fibronectin (2000) Biomaterials, 21 (15), pp. 1541-1547 Cosgaya, J.M., Chan, J.R., Shooter, E.M., The neurotrophin receptor p75NTR as a positive modulator of myelination (2002) Science, 298, p. 1245 Cruz, N.I., Debs, N., Fiol, R.E., Evaluation of fibrin glue in rat sciatic nerve repairs (1986) Plast. Reconstr. Surg., 78 (6), pp. 369-373 da Silva, C.F., da Gama, S.A., Júnior, R.M., Pereira, F.C., Influence of highly purified of hyaluronic acid on peripheral nerve regeneration in vivo (2003) Braz. J. Morfol. Sci., 20 (2), pp. 121-124 Delorme, B., Ringe, J., Gallay, N., Levern, Y., Kerboeuf, D., Jorgensen, C., Rosset, P., Charbord, P., Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells (2008) Blood, 111, pp. 2631-2635 Dominici, M., Le Blanc, K., Mueller, I., Slaper-Cortenbach, I., Marini, F., Krause, D., Deans, R., Horwitz, E., Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy Position Statement (2006) Cytotherapy, 8, pp. 315-317 Evans, G.R., Brandt, K., Katz, S., Chauvin, P., Otto, L., Bogle, M., Wang, B., Patrick, C.W., Bioactive poly (l-lactic acid) conduits seed with Schwann cells for peripheral nerve regeneration (2002) Biomaterials, 23 (3), pp. 841-848 Fields, R.D., Le Beau, J.M., Longo, F.M., Ellisman, M.H., Nerve regeneration through artificial tubular implants (1989) Prog. Neurobiol., 33 (2), pp. 87-134 Freria, C.M., Barbizan, R., Oliveira, A.L.R., Granulocyte colony stimulating factor neuroprotective effects on spinal motoneurons after ventral root avulsion (2012) Synapse, 66, pp. 128-141 Gasparotto, V.P.O., Landim-Alvarenga, F.C., Oliveira, A.L.R., Simões, G.F., Lima-Neto, J.F., Barraviera, B., Ferreira, R.S., A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells (2014) Stem Cell Res. Ther., 5, p. 78 Gille, J., Meisner, U., Ehlers, E.M., Muller, A., Russlies, M., Behrens, P., Migration pattern, morphology and viability of cells suspended in or sealed with fibrin glue: a histomorphologic study (2005) Tissue Cell, 37 (5), pp. 339-348 Hakamata, Y., Tahara, K., Uchida, H., Sakuma, Y., Nakamura, M., Kume, A., Murakami, T., Kobayashi, E., Green fluorescent protein-transgenic rat: a tool for organ transplantation research (2001) Biochem. Biophys. Res. Commun., 286 (4), pp. 779-785 Ide, C., Peripheral nerve regeneration (1996) Neurosci. Res., 25 (2), pp. 101-121 Labrador, R.O., Butí, M., Navarro, X., Influence of collagen and laminin gels concentration nerve regeneration after resection and tube repair (1998) Exp. Neurol., 149 (1), pp. 243-252 Labrador, R.O., Buti, M., Navarro, X., Peripheral nerve repair: role of agarose matrix density on functional recovery (1995) Neuroreport, 6 (15), pp. 2022-2026 Ladak, A., Olson, J., Tredget, E.E., Gordon, T., Differentiation of mesenchymal stem cells to support nerve regeneration in a rat model (2011) Exp. Neurol., 228, pp. 242-252 Lundborg, G., Rosen, B., Abrahamson, S.O., Dahlin, L., Danielsen, N., Tubular repair of the median nerve in the human forearm. Preliminary findings (1994) J. Hand Surg., 19, pp. 273-276 Lundborg, G., Rosen, L., Dahlin, L., Holmberg, J., Rosen, I., Tubular repair of the median or ulnar nerve in the human forearm: a 5-year follow-up (2004) J. Hand Surg., 29, pp. 100-107 Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res., 254, pp. 317-330 Mayhew, T.M., Sharma, A.K., Sampling schemes for estimating nerve fibre size. II. Methods for unifascicular nerve trunks (1984) J. Anat., 139, pp. 59-66 Morales, M.M., (2007) Terapias avançadas: células tronco, terapia gênica e nanotecnologia aplicada à saúde, , Atheneu, São Paulo Navarro, X., Verdú, E., Rodriguez, F.J., Ceballos, D., Artificial nerve graft for the repair of peripheral nerve injuries (2001) NeurolSci., 22, pp. S7-S13 Oliveira, A.L.R., Pierucci, A., Pereira, K.B., Review: peripheral nerve through the nerve tubulization technique (2004) Braz. J. Morphol. Sci., 21 (4), pp. 225-231 Pereira Lopes, F.R., Campos, L.C.M., Corrêa, J.D., Balduino, A., Lora, S., Langone, F., Borojevic, R., Martinez, A.M.B., Bone marrow stromal cells and resorbable collagen guidance tubes enhance sciatic nerve regeneration in mice (2006) Exp. Neurol., 198 (2), pp. 457-468 Pierucci, A., Faria, A.M., Pimentel, E.R., Santos, A.R., Oliveira, A.L.R., Effects of agrecan on Schwann cell migration in vitro and nerve regeneration in vivo (2004) Braz. J. Morphol. Sci., 21 (3), pp. 125-130 Prockop, D.J., "Stemness" does not explain the repair of many tissues by mesenchymal stem/multipotent stromal cells (2007) Clin. Pharmacol. Ther., 82, pp. 241-243 Rodrígues, F.J., Verdú, E., Ceballos, D., Navarro, X., Nerve guides seeded with autologous Schwann cells improve nerve regeneration (2000) Exp. Neurol., 161 (2), pp. 571-584 Schlosshauer, B., Muller, E., Schroder, B., Planck, H., Muller, H.W., Rat Schwann cells in bioresorbable nerve guides to promote and accelerate axonal regeneration (2003) Brain Res., 963 (1-2), pp. 321-326 Schipani, E., Kronenberg, H.M., Adult mesenchymal stem cells (2009) Stem Book, , http://www.stembook.org/node/538, Harvard Cell Institute, Cambrige, MA Schmidt, C.E., Leach, J.B., Neural tissue engineering: strategies for repair and regeneration (2003) Annu. Rev. Biomed. Eng., 5, pp. 293-347 Silver, F.H., Wang, M.C., Pins, G.D., Preparation and use of fibrin glue in surgery (1995) Biomaterials, 16 (12), pp. 891-903 Thomazini-Santos, I.A., Barraviera, S.R.C.S., Mendes-Giannini, M.J.S., Barraviera, B., Surgical adhesives (2001) J. Venom Anim. Toxins, 7 (2), pp. 159-171 Tolwani, R.J., Cosgaya, J.M., Varma, S., Jacob, R., Kuo, L.E., Shooter, E.M., BDNF overexpression produces a long-term increase in myelin formation in the peripheral nervous system (2004) J. Neurosci. Res., 77, pp. 662-669 Tong, X.J., Hirai, K., Shimada, H., Mizutani, Y., Izumi, T., Toda, N., Yu, P., Sciatic nerve regeneration navigated by laminin-fibronectin double coated biodegradable collagen grafts in rats (1994) Brain Res., 663 (1), pp. 155-162 Ucelli, A., Moretta, L., Pistora, V., Mesenchymal stem cells in healthy and disease (2008) Nat. Rev. Immunol., 8, pp. 726-736 Victório, S.C., Cartarozzi, L.P., Hell, R.C., Oliveira, A.L.R., Decreased MHC I expression in IFN gamma mutant mice alters synaptic elimination in the spinal cord after peripheral nerve injury (2012) J. Neuroinflammation, 9, p. 88 Wang, J., Ding, F., Gu, Y., Liu, J., Gu, X., Bone marrow mesenchymal stem cells promote cell proliferation and neurotophic function of Schwann cells in vitro and in vivo (2009) Brain Res., 1262, pp. 7-15 Wennersten, A., Holmin, S., Al Nimer, F., Meijer, X., Wahlberg, L.U., Mathiesen, T., Sustained survival of xenografted human neural stem/progenitor cells in experimental brain trauma despite discontinuation of immunosuppression (2006) Exp. Neurol., 199 (2), pp. 339-347 Yannas, I.V., Hill, B.J., Selection of biomaterials for peripheral nerve regeneration using data from the nerve chamber model (2004) Biomaterials, 25 (9), pp. 1593-1600 Zanon, R.G., Cartarozzi, L.P., Victório, S.C., Moraes, J.C., Morari, J., Velloso, L.A., Oliveira, A.L., Interferon (IFN) beta treatment induces major histocompatibility complex (MHC) class I expression in the spinal cord and enhances axonal growth and motor function recovery following sciatic nerve crush in mice (2010) Neuropathol. Appl. Neurobiol., 36 (6), pp. 515-534 Zochodne, D.W., The challenges and beauty of peripheral nerve regrowth (2012) J. Periph. Nerv. Sys., 17, pp. 1-18