Artículos de revistas
A New Amperometric Biosensor For Salicylate Based On Salicylate Hydroxylase Immobilized On Polipyrrole Film Doped With Hexacyanoferrate
Registro en:
Analytica Chimica Acta. , v. 347, n. 01/02/15, p. 35 - 41, 1997.
32670
10.1016/S0003-2670(97)00105-0
2-s2.0-0030842924
Autor
Milagres B.G.
De Oliveira Neto G.
Kubota L.T.
Yamanaka H.
Institución
Resumen
An amperometric biosensor for salicylate detection was developed by immobilizing salicylate hydroxylase via glutaraldehyde onto a polypyrrole film doped with hexacyanoferrate, supported on a glassy carbon electrode surface. The sensor monitors the catechol produced in the enzymatic reaction on the film surface, at an applied potential of 150 mV vs. SCE. A [NADH]/[salicylate] ratio between 2 and 4 gave the best response. The biosensor presented the best performance in a solution with pH=7.4. The response time was about 40 s. A linear range of response was observed for salicylate concentrations between 1.0x10-5 and 1.0x10-4 mol l-1 and the equation adjusted for this curve was I=(-0.04±0.01)+(11.4±0.2)[salicylate] with a correlation coefficient of 0.999 for n=6. The biosensor retains its activity for at least 10 days despite daily use. The results obtained using the biosensor for salicylate determination, in three different samples of antithermic drugs, presented a good correlation with the standard colorimetric method. 347 01/02/15 35 41 Frew, J.E., Bayliff, S.W., Gibbs, P.N.B., Green, M.J., (1989) Anal. Chim. Acta, 273, p. 39 Rainsdorf, K.D., (1984) Aspirin and the Salicylates, pp. 56-58. , Butterworths, London, UK Chem, B.E., Johns, D., Bochner, F., Imhoff, O.M., Rowland, M., (1979) Clin. Chem., 25, p. 1420 Kwong, T.C., (1985) Clin. Chem. News, 1, p. 14 Kang, E.S., Todd, T.A., Capaci, M.T., Schwenzer, K., Jabbour, J.T., (1983) Clin. Chem., 29, p. 1012 Stewart, M.J., Watson, I.D., (1987) Ann. Clin. Biochem., 24, p. 552 Rivas, G.A., Calatayud, J.M., (1995) Talanta, 42, p. 1285 Bertocchi, P.B., D'Ottavio, D., Evangelist, M.E., Mascini, M., Palleschi, G., (1992) Clin. Chim. Acta, 207, p. 205 You, K.S., Bitticofer, J.A., (1984) Clin. Chem., 30, p. 1549 Saha, U., Baksi, K., (1985) Analyst, 110, p. 739 Pulgarin, J.A.M., Molina, A.A., (1994) Analyst, 119, p. 1915 Villari, A., Micali, N., Frest, M., Puglisi, G., (1994) Analyst, 119, p. 1561 Carlson, M., Thompson, R.D., (1987) J. Liquid Chromathogr., 10, p. 997 Buskin, J.N., Upton, R.A., William, R.L., (1982) Clin. Chem., 28, p. 1200 Papaglou, A.M., Diamandis, E.P., Hadjiioannou, T.P., (1984) Anal. Chim. Acta, 159, p. 393 Hassan, S.M., Hamada, M.A., (1988) Analyst, 113, p. 1709 Gilmartin, M.A.T., Hart, J.P., (1995) Anal. Proc., 32, p. 341 Moore, T.J., Joseph, M.J., Allen, B.W., Coury, L.A., (1995) Anal. Chem., 67, p. 1896 Neumayr, M., Friedrich, O., Sontag, G., (1993) Anal. Chim. Acta, 273, p. 469 Rahni, M.A.N., Guilbault, G.G., De Oliveira Neto, G., (1986) Anal. Chim. Acta, 181, p. 219 Gorton, L., Bremle, G., Csoregi, E., Pettersson, G.J., Persson, B., (1991) Anal. Chim. Acta, 249, p. 43 You, K.S., (1985) Clin. Chim. Acta, 149, p. 281 Neumayr, M., Sontag, G., Pittner, F., (1995) Anal. Chim. Acta, 305, p. 26 Fonong, T., Rechnitz, G.A., (1984) Anal. Chim. Acta, 158, p. 357 Gorton, L., (1995) Electroanalysis, 7, p. 23 Gorton, L., Karan, H.J., Hale, P.D., Inagaki, T., Okamoto, Y., Skothein, T.A., (1990) Anal. Chim. Acta, 228, p. 23 Wang, J., Wu, L.H., Lu, Z., Li, R., Sanches, J., (1990) Anal. Chim. Acta, 228, p. 251 Borges, C.G., De Oliveira Neto, G., Kubota, L.T., Grandin, L., (1996) J. Electroanal. Chem., 418, p. 147 Kubota, L.T., Milagres, B.G., Gouveia, F., De Oliveira Neto, G., (1996) Anal. Lett., 29, p. 893 Dong, S., Ding, J., (1988) Synth. Metals, 24, p. 273 Van Dyke, L.S., Martin, C.R., (1990) Synth. Metals, 36, p. 275 Lever, A.B.P., (1990) Inorg. Chem., 29, p. 1271 Murray, R.W., Chemically modified electrodes (1984) Electroanalytical Chemistry, 13, pp. 191-368. , A.J. Bard (Ed.), Marcel Dekker Inc., NY Choi, K.K., Fung, K.W., (1982) Anal. Chim. Acta, 138, p. 385