Artículos de revistas
Morphological Segmentation Of Yeast By Image Analysis
Registro en:
Image And Vision Computing. , v. 25, n. 1, p. 34 - 39, 2007.
2628856
10.1016/j.imavis.2006.01.006
2-s2.0-33750606683
Autor
de Carvalho M.A.G.
Lotufo R.d.A.
Couprie M.
Institución
Resumen
An image analysis method has been developed to segment yeast cells. Yeasts belong to the taxonomic group fungi and have been used on fuel and food industry, for example. The method is capable of segmenting yeast cells based on Watershed Transform and space-scale analysis of the Tree of Critical Lakes. We analise hierarchical, geometric and gray-scale properties of the Tree of Critical Lakes. We show experimental results for one group of yeast images obtained from the School ofFood Engineering at Unicamp, Brazil. Comparison shows that the proposed method provides cells with area 10% lower than traditional approach. Moreover, this approach preserves the cells contour, an important feature because of the performance of bioreactors and other chemical processes are greatly influenced by their morphological character. © 2006 Elsevier B.V. All rights reserved. 25 1 34 39 Kurtzman, C.P., Fell, J.W., (1998) The Yeasts-A Taxonomic Study, , Elsevier, Amsterdam O'Shea, D.G., Walsh, P.K., Morphological characterization of the dimorphic yeast Kluyveromyces marxianus var. marxianus NRRLy2415 by semi-automated image analysis (1996) Biotechnology and Bioengineering, 51, pp. 679-690 O'Shea, D.G., Walsh, P.K., The effect of the culture conditions on the morphology of the dimorphic yeast Kluyveromyces marxianus var. marxianus NRRLy2415: a study incorporating image analysis (2000) Applied Microbiology and Biotechnology, 53, pp. 316-322 Zalewski, K., Buchholz, R., Morphological analysis of yeast cells using an automated image processing system (1996) Journal of Biotechnology, 48, pp. 43-49 Pons, M.N., Vivier, H., Rémy, J.F., Dodds, J.A., Morphological characterization of yeast by image analysis (1993) Biotechnology and Bioengineering, 42, pp. 1352-1359 Beucher, S., Meyer, F., The morphological approach to segmentation: the watershed transformation (1993) Mathematical Morphology in Image Processing, pp. 433-481. , Dougherty E. (Ed), Marcel Dekker, New York (Chapter 12) Meyer, F., The dynamics of minima and contours (1996) Mathematical Morphology and its Applications to Image and Signal Processing, pp. 329-336. , Maragos P., Schafer R.W., and Butt M.A. (Eds), Kluwer Academic Publishers, Dordrecht Cutsem, B.V., Yeart, B., Indexed dendrograms on random dissimilarities (1998) Journal of Classification, 15, pp. 93-127 Fitzgibbon, A.W., Pilu, M., Fisher, R.B., Direct least squares fitting of ellipses (1999) IEEE Transactions on Pattern Analysis and Machine Intelligence, 21 (5), pp. 476-480