Artículos de revistas
Optimization Of A Method For Determination Of Flavonols And Flavones In Fruits By Hplc Using Statistical Design And Response Surface Analysis [otimização De Método Para Determinação De Flavonóis E Flavonas Em Frutas Por Cromatografia Líquida De Alta Eficiência Utilizando Delineamento Estatístico E Análise De Superfície De Resposta]
Registro en:
Quimica Nova. , v. 31, n. 6, p. 1378 - 1384+S1-S2, 2008.
1004042
10.1590/S0100-40422008000600020
2-s2.0-54549095987
Autor
Hoffmann-Ribani R.
Rodriguez-Amaya D.B.
Institución
Resumen
This work optimized the HPLC conditions for the simultaneous determination of luteolin, apigenin, myricetin, quercetin and kaempferol in aglycone form, as well defined the best conditions for hydrolysis/extraction of these flavonoids in fruits, using the statistical central composite design and response surface analysis. A reverse phase method was developed using a gradient of methanol/water acidified with 0.3% formic acid as mobile phase and a photodiode array detector. The samples were extracted with methanol/water (50:50 v/v) at 90̊C. The optimum time and HCl concentration varied for the different fruits investigated, demonstrating the necessity of optimizing these conditions for each fruit analyzed. Good recovery (87.1 to 96.3%), repeatability and linearity were obtained. 31 6 1378 1384+S1-S2 Armstrong, B.K., Mann, J.L., Adelstein, A.M., Eskin, F., (1975) J. Chron. Dis, 28, p. 455 Block, G., Patterson, B., Subar, A., (1992) Nutr. Cancer, 18, p. 1 Burr, M.C., Sweetnam, P.M., (1982) Am. J. Clin. Nutr, 36, p. 673 Phillips, R.L., Lemon, F.R., Beeson, W.L., Kuzma, J.W., (1978) Am. J. Clin. Nutr, 31, p. 191 Verlangieri, A.J., Kapeghian, J.C., El-Dean, S., Bush, M., (1985) Med. Hypotheses, 16, p. 7 Castellucio, C., Paganga, G., Melikian, N., Bolwell, G.P., Pridham, J., Sampson, J., Rice-Evans, C., (1995) FEBS Lett, 368, p. 188 Hertog, M.G.L., Feskens, E.J.M., Hollman, P.C.H., Katan, M.B., Kromhout, D., (1993) Lancet, 342, p. 1007 Keli, S.O., Hertog, M.G.L., Feskin, E.J.M., Kromhout, D., (1996) Arch. Int. Med, 156, p. 632 Knekt, P., Jarvinen, R., Reunanen, A., Maatela, J., (1996) Br. Med. J, 312, p. 478 Middleton, E., Rice-Evans, C., Miller, N.J., Bolwell, G.P., Bramley, P.M., Pridham, J.B., (1995) Free Radical Res, 22, p. 375 Rimm, E.B., Katan, M.B., Ascherio, A., Stampher, M.J., Willet, W.C., (1996) Ann. Int. Med, 125, p. 384 Kühnau, J., (1976) World Rev. Nutr. Diet, 24, p. 117 Markham, K.R., (1989) Meth. Plant Biochem, 1, p. 197 Williams, C. A. Harborne, J. B. Em The Flavonoids. Advances in Research since 1986 Harborne, J.B., ed. Chapman & Hall: London, 1994Hertog, M.G.L., Hollman, P.C.H., Venema, D.P., (1992) J. Agric. Food Chem, 40, p. 1591 Haaland, P.O., (1989) Experimental Design in Biotechnology, , Marcel Dekker: New York Box, G.E.P., Wilson, K.B., (1951) J. Roy. Statist. Soc, B13, p. 1 Myers, R.H., Montgomery, D.C., (2002) Response Surface Methodology: Process and Product Optimization using Designed Experiments, , 2nd ed, Wiley: New York Barros Neto, B. Scarminio, I. S. Bruns, R. E. Planejamento e Otimização de Experimentos, 2a ed., Educamp: Campinas, 1995Cacace, J.E., Mazza, G., (2003) J. Food Sci, 68, p. 240 Careri, M., Elviri, L., Mangia, A., Musci, M., (2000) J. Chromatogr., A, 881, p. 449 Catharino, R.R., Godoy, H.T., (2001) Ciênc. Tecnol. Aliment, 21, p. 326 Liyana-Pathirana, C., Shahidi, F., (2005) Food Chem, 93, p. 47 Arabbi, P.R., Genovese, M.I., Lajolo, F.M., (2004) J. Agric. Food Chem, 52, p. 1124 Franke, A.A., Custer, L.J., Arakaki, C., Murphy, S.P., (2004) J. Agric. Food Chem, 17, p. 1 Häkkinen, S., Törrönen, R., (2000) Food Res. Int, 33, p. 517 Hertog, M.G.L., Hollman, P.C.H., Katan, M.B., (1992) J. Agric. Food Chem, 40, p. 2379 Shabir, G.A., (2003) J. Chromatogr., A, 987, p. 57 Franke, A.A., Custer, L.J., Arakaki, C., Murphy, S.P., (2004) J. Agric. Food Chem, 17, p. 1 Häkkinen, S., Törrönen, R., (2000) Food Res. Int, 33, p. 517 Hertog, M.G.L., Hollman, P.C.H., Katan, M.B., (1992) J. Agric. Food Chem, 40, p. 2379