A new genus, Cleistesiopsis, was segregated from Cleistes based on morphological and molecular characteristics, and two new species of Cleistes: C. batistana and C. elongata, both occurring in the Brazilian Central Plateau (Central-Western Brazil), are described and illustrated. Furthermore, a key to genera currently recognised within Pogonieae is presented. © 2008 The Board of Trustees of the Royal Botanic Gardens.633441448Ames, O., (1922) Orchidaceae, Illustrations and Studies of the Family Orchidaceae Issuing from the Ames Botanical Laboratory North Easton, 7, p. 21. , Massachussetts, Boston, New York The Merrymount Press, BostonBrown, P.M., (1995) N. Amer. Native Orchid J., 11, p. 8Brown, P.M., Wunderlin, R.P., A new combination in the genus Pogonia (1997) N. Amer. Native Orchid J., 3, pp. 450-452. , 4Brown, R., (1913) Hortus Kewensis Ed. 2, 5. , W. T. Aiton. Longman, Hurst, Rees, Orme, & Brown, LondonCameron, K.M., Phylogeny and biogeography of Pogoniinae (Vanilloideae: Orchidaceae) (1999) North Amer. Nat. Orchid J., 5, pp. 152-163Cameron, K.M., Pridgeon, A.M., Cribb, P.J., Chase, M.W., Rasmussen, F.N., Vanilloideae (2003) Genera Orchidacearum, 3. , Oxford University Press OxfordCameron, K.M., Utility of plastid psaB gene sequences for investigating intrafamilial relationships within Orchidaceae (2004) Molec. Phylogenet. Evol., 31, pp. 1157-1180Cameron, K.M., Chase, M.W., Seed morphology of vanilloid orchids (Vanilloideae: Orchidaceae) (1998) Lindleyana, 13, pp. 148-169Cameron, K.M., Chase, M.W., Phylogenetic relationships of Pogoniinae (Vanilloideae, Orchidaceae): An herbaceous example of the eastern North America - Eastern Asia phytogeographic disjunction (1999) J. Plant Res., 112, pp. 317-329Cameron, K.M., Chase, M.W., Whitten, W.M., Kores, P.J., Jarrell, D.C., Albert, V.A., Yukawa, T., Goldman, D.H., A phylogenetic analysis of the Orchidaceae: Evidence from rbcL nucleotide sequences (1999) Amer. J. Bot., 86, pp. 208-224Catling, P.M., Gregg, K.B., Systematics of the genus Cleistes in North America (1992) Lindleyana, 7, pp. 57-73Chase, M.W., Cameron, K.M., Barrett, R.L., Freudenstein, J.V., Dixon, K.W., Kell, S.P., Barrett, R.L., Cribb, P.J., DNA data and Orchidaceae systematics: A new phylogenetic classification (2003) Orchid Conservation, , Natural History Publications (Borneo) Kota KinabaluDressler, R.L., (1993) Phylogeny and Classification of the Orchid Family, , Cambridge University Press CambridgeFernald, M.L., Contributions from the Gray Herbarium of Havard University - No. CLXII. Identifications and reidentifications of North American Plants (1946) Rhodora, 48, pp. 184-197Freudenstein, J.V., Van Den Berg, C., Goldman, D.H., Kores, P.J., Molvray, M., Chase, M.W., An expanded plastid DNA phylogeny of Orchidaceae and analyses of Jackknife branch support strategy (2004) Amer. J. Bot., 91, pp. 149-157Gregg, K.B., Reproductive biology of the orchid Cleistes divaricata (L.) Ames var. bifaria Fernald growing in a West Virginia meadow (1989) Castanea, 54, pp. 57-78Gregg, K.B., Reproductive strategy of Cleistes divaricata (Orchidaceae) (1991) Amer. J. Bot., 78, pp. 350-360Gregg, K.B., Defrauding the deceitful orchid: Pollen collection by pollinators of Cleistes divaricata and C. bifaria (1991) Lindleyana, 6, pp. 214-220Harris, J.G., Harris, M.W., (1994) Plant Identification Terminology: An Illustrated Glossary, , Spring Lake Publishing Spring LakeLinnaeus, (1753) Species Plantarum. L., , Salvii, StockholmPansarin, E.R., Biologia floral de Cleistes macrantha (Barb. Rodr.) Schltr. (Orchidaceae: Vanilloideae: Pogoniinae) (2003) Rev. Brasil. Bot., 26, pp. 73-80Pansarin, E.R., Cleistes pusilla: A new species from Central Brazil (2004) Kew Bull., 59, pp. 555-558Pansarin, E.R., (2005) Sistemática Filogenética e Biologia Floral de Pogoniinae Sul - Americanas, e Revisão Taxonômica e Análise Das Ceras Epicuticulares Do Gênero Cleistes Rich. Ex Lindl. (Orchidaceae), , PhD Thesis. Universidade Estadual de Campinas CampinasPansarin, E.R., Salatino, A., Salatino, M.L.F., Phylogeny of South American Pogonieae (Orchidaceae, Vanilloideae) based on sequences of nuclear ribosomal (ITS) and chloroplast (psaB, rbcL rps16, and trnL-F) DNA with emphasis on Cleistes and discussion of biogeographic implications (2008) Org. Divers. Evol., 8, pp. 171-181