Artículos de revistas
Effect Of Betamethasone And Diclofenac Sodium On Serum And Tissue Concentration Of Amoxicillin. In Vivo Study In Rats
Journal Of Applied Oral Science. , v. 14, n. 5, p. 319 - 323, 2006.
Mattos Filho T.R.
Objective: Antimicrobial agents in combination with anti-inflammatory drugs have been usually prescribed in both Medicine and Dentistry. However, few scientific reports support this clinical practice. The aim of this study was to evaluate the effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin in rats. Methods: Four polyurethane sponges were implanted in the back skin of 48 rats. After seven days the animals were divided into 6 groups (n=8). Group 1: amoxicillin (25 mg/kg); G2: diclofenac sodium (2.5 mg/kg); G3: betamethasone (0.1 mg/kg); G4: diclofenac sodium and amoxicillin; G5: betamethasone and amoxicillin; and G6: 0.9% sodium chloride solution (1.0 mL - control group). All drugs were administered in a single dose. After 90 minutes, the granulomatous tissues of each animal were surgically removed and weighed. Blood was collected from cervical plexus, centrifuged and 10μL of serum was placed on paper discs. In order to estimate amoxicillin concentration, serum and granulomatous tissues were separately submitted to microbiological assay, which used 108cfu/mL of Staphylococcus aureus ATCC 25923 (penicillin-susceptible strain). After incubation (18 hours, 37°C) the inhibition zones were measured and compared to a regression curve. Results: No inhibition zones were observed for groups 2, 3 and 6. Tissue and serum concentrations of both G1 (4.14μ g/g and 2.06μ g/mL, respectively) and G5 (3.87μ g/g and 1.70μ g/mL, respectively) showed statistically significant differences (Kruskal-Wallis, p<0.05) in comparison to G4 (1.45μ g/g and 0.41μ g/mL, respectively). G1 and G5 did not differ significantly (p>0.05). Conclusion: Considering single doses, betamethasone did not interfere with amoxicillin levels but diclofenac sodium reduced both tissue and serum levels of amoxicillin in rats.145319323Agarwal, G., Awasthi, S., Kabra, S.K., Kaul, A., Singhi, S., Walter, S.D., Three day versus five day treatment with amoxicillin for non-severe pneumonia in young children: A multicentre randomised controlled trial (2004) BMJ, 328, p. 791Alexander, R.E., Throndson, R.R., A review of perioperative corticosteroid use in dentoalveolar surgery (2000) Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 90, pp. 406-415Baglie S, Groppo FC, Mattos-Filho TR. Tissue pharmacokinetic of amoxicillin. An experimental design in rats. Braz J Infect Dis. 2000;4:197-203Bantar, C., Nicola, F., Fernandez Canigia, L., Arenoso, H.J., Soutric, J., Montoto, M., A pharmacodynamic model to support a 12-hour dosing interval for amoxicillin/sulbactam, a novel oral combination, in the treatment of community-acquired lower respiratory tract infections (2000) J Chemother, 12, pp. 223-227Charles, B.G., Chulavatnatol, S., Simple analysis of amoxicillin in plasma by high performance liquid chromatography whit internal standardization and ultraviolet detection (1993) Biomed Chromatogr, 7, pp. 204-207Dajani, A.S., Bawdon, R.E., Berry, M.C., Oral Amoxicillin as prophylaxis for endocarditis: What is the optimal dose? (1994) Clin Infect Dis, 18, pp. 157-160Dajani, A.S., Taubert, K.A., Wilson, W., Bolger, A.F., Bayer, A., Ferrieri, P., Prevention of bacterial endocarditis: Recommendations by the American Heart Association (1997) J Am Dent Assoc, 128, pp. 1142-1151Garbutt, J.M., Goldstein, M., Gellman, E., Shannon, W., Littenberg, B., A randomized, placebo-controlled trial of antimicrobial treatment for children with clinically diagnosed acute sinusitis (2001) Pediatrics, 107, pp. 619-625Georgopoulos, A., Borek, M., Ridl, W., Randomized, double-blind, double-dummy study comparing the efficacy and safety of amoxycillin 1 g bd with amoxycillin 500 mg tds in the treatment of acute exacerbations of chronic bronchitis (2001) J Antimicrob Chemother, 47, pp. 67-76Gordon, R.C., Regamey, C., Kirby, W.M.N., Comparative clinical pharmacology of amoxicillin and ampicillin administered orally (1972) Antimicrob Agents Chemother, 1, pp. 504-507Groppo, F.C., Mattos-Filho, T.R., Del-Fiol, F.S., Bioassay of amoxicillin in rats (2000) Biol Pharm Bull, 23, pp. 1033-1035Groppo, F.C., Simões, R.P., Ramacciato, J.C., Rehder, V., Andrade, E.D., Mattos-Filho, T.R., Effect of sodium diclofenac on serum and tissue concentration of amoxicillin and on staphylococcal infection (2004) Biol Pharm Bull, 27, pp. 52-55Huisman-de-Boer, J.J., van den Anker, J.N., Vogel, M., Goessens, W.H., Schoemaker, R.C., Groot, R., Amoxicillin pharmacokinetics in preterm infants with gestational ages of less than 32 weeks (1995) Antimicrob Agents Chemother, 39, pp. 431-434Koneman, E.W., Allen, S.D., Janda, W.M., Schreckenberger, P.C., Winn Jr., W.C., (1997) Color atlas and textbook of diagnostic microbiology, , Philadelphia: J.B. Lippincott Company;Margarit, F., Moreno-Dalmau, J., Obach, R., Peraire, C., Pla-Delfina, J.M., Intestinal absorption kinetics of a series of aminopenicillins and azidocillin. A comparative study in the rat (1991) Eur J Drug Metab Pharmacokinet, 3, pp. 102-107Martindale, W., (1996) The Extra Pharmacopoeia, pp. 125-129. , 31th ed. London: Royal Pharmaceutical Society PressMoore, T.D., Horton, R., Utrup, L.J., Miller, L.A., Poupard, J.A., Stability of amoxicilin-clavulanate in bactec medium determined by high-performance liquid chromatography and bioassay (1996) J Clin Microbiol, 34, pp. 1321-1322Moore, V.A., Irwin, W.J., Timmins, P., Chong, S., Dando, S.A., Morrison, R.A., A rapid screening system to determine drug affinities for the intestinal dipeptide transporter 1: System characterization (2000) Int J Pharm, 210, pp. 15-27Park, S.N., Yeo, S.W., Effects of antibiotics and steroid on middle ear mucosa in rats with experimental acute otitis media (2001) Acta Otolaryngol, 121, pp. 808-812Peris-Ribera, J.E., Torres-Molina, F., Garcia-Carbonell, M.C., Aristorena, J.C., Pla-Delfina, J.M., Pharmacokinetics and bioavailability of diclofenac in the rat (1991) J Pharmacokinet Biopharm, 19, pp. 647-665Plant, J.E., Higgs, G.A., Easmon, C.S.F., Effects of anti-inflammatory agents on chronic Salmonella typhimurium infection in a mouse model (1983) Infect Immun, 42, pp. 71-75Prevot, M.H., Jehl, F., Rouveix, B., Pharmacokinetics of a new oral formulation of amoxicillin (1997) Eur J Drug Metab Pharmacokinet, 22, pp. 47-52Rodriguez, A.F., Kaplan, S.L., Hawkins, E.P., Mason Jr., E., Effect of dexamethasone or hwa-138 in combination with antibiotics in experimental haemophilus influenzae type b infection (1991) Antimicrob Agents Chemother, 35, pp. 1980-1984Scholer, D.W., Ku, E.C., Boettcher, I., Schweizer, A., Pharmacology of diclofenac sodium (1986) Am J Med, 80, pp. 34-38Siaperas, P., Pefanis, A., Iliopoulos, D., Katsarolis, I., Kyroudi-Voulgari, A., Donta, I., Evidence of less severe aortic valve destruction after treatment of experimental staphylococcal endocarditis with vancomycin and dexamethasone (2001) Antimicrob Agents Chemother, 45, pp. 3531-3537Sugawara, M., Saitoh, H., Iseki, K., Miyazaki, K., Arita, T., Contribution of passive transport mechanisms to the intestinal absorption of betalactam antibiotics (1989) J Pharm Pharmacol, 42, pp. 314-318Terada, T., Saito, H., Mukai, M., Inui, K.I., Recognition of beta-lactam antibiotics by rat peptide transporters, PEPT1 and PEPT2, in LLC-PK1cells (1997) Am J Physiol, 273, pp. 706-711Van Pelt, L.F., Ketamine and xylazine for surgical anesthesia in rats (1977) J Am Vet Med Assoc, 171, pp. 842-844Verba, V., Sakiniene, E., Tarkowski, A., Beneficial effect of glucocorticoids on the course of haematogenously acquired Staphylococcus aureus nephritis (1997) Scand J Immunol, 45, pp. 282-286Westphal, J.F., Brogard, J.M., Jehl, F., Carbon, C., Assessment of the role of enterocyte ion exchange in the intestinal absorption of amoxicillin, based on a study of the interaction with amiloride (1995) Pathol Biol, 43, pp. 590-595Yoshizumi, O.M., Lee, G.C., Equi, R.A., Kim, I.T., Pitchekian-Halabi, H., Adamu, S.A., Timing of dexamethasone treatment in experimental Staphylococcus aureus endophthalmitis (1998) Retina, 18, pp. 130-135