Artículos de revistas
Enhanced Calcium Mobilization In Rat Ventricular Myocytes During The Onset Of Pressure Overload-induced Hypertrophy
Registro en:
American Journal Of Physiology - Heart And Circulatory Physiology. , v. 291, n. 4, p. H1803 - H1813, 2006.
3636135
10.1152/ajpheart.01345.2005
2-s2.0-33749362362
Autor
Carvalho B.M.R.
Bassani R.A.
Franchini K.G.
Bassani J.W.M.
Institución
Resumen
Early cardiovascular changes evoked by pressure overload (PO) may reveal adaptive strategies that allow immediate survival to the increased hemodynamic load. In this study, systolic and diastolic Ca 2+ cycling was analyzed in left ventricular rat myocytes before (day 2, PO-2d group) and after (day 7, PO-7d group) development of hypertrophy subsequent to aortic constriction, as well as in myocytes from timematched sham-operated rats (sham group). Ca 2+ transient amplitude was significantly augmented in the PO-2d group. In the PO-7d group, intracellular Ca 2+ concentration ([Ca 2+] i) was reduced during diastole, and mechanical twitch relaxation (but not [Ca 2+] i decline) was slowed. In PO groups, fractional sarcoplasmic reticulum (SR) Ca 2+ release at a twitch, SR Ca 2+ content, SR Ca 2+ loss during diastole, and SR-dependent integrated Ca 2+ flux during twitch relaxation were significantly greater than in sham-operated groups, whereas the relaxation-associated Ca 2+ flux carried by the Na +/Ca 2+ exchanger was not significantly changed. In the PO-7d group, mRNA levels of cardiac isoforms of SR Ca 2+-ATPase (SERCA2a), phospholamban, calsequestrin, ryanodine receptor, and NCX were not significantly altered, but the SERCA2a-to-phospholamban ratio was increased 2.5-fold. Moreover, greater sensitivity to the inotropic effects of the β-adrenoceptor agonist isoproterenol was observed in the PO-7d group. The results indicate enhanced Ca 2+ cycling between SR and cytosol early after PO imposition, even before hypertrophy development. Increase in SR Ca 2+ uptake may contribute to enhancement of excitation-contraction coupling (augmented SR Ca 2+ content and release) and protection against arrhythmogenesis due to buildup of [Ca 2+] i during diastole. Copyright © 2006 the American Physiological Society. 291 4 H1803 H1813 Akers, W.S., Cross, A., Speth, R., Dwoskin, L.P., Cassis, L.A., Reninangiotensin and sympathetic nervous system in cardiac pressure-overload hypertrophy (2000) Am J Physiol Heart Circ Physiol, 279, pp. H2797-H2806 Anger, M., Lompré, A.M., Vallot, O., Marotte, F., Rappaport, L., Samuel, J.L., Cellular distribution of Ca 2+ pumps and Ca 2+ release channels in rat cardiac hypertrophy induced by aortic stenosis (1998) Circulation, 98, pp. 2477-2486 Anversa, P., Ricci, R., Olivetti, G., Quantitative structural analysis of the myocardium during physiologic growth and induced cardiac hypertrophy (1986) J Am Coll Cardiol, 7, pp. 1140-1149 Arai, M., Suzuki, T., Nagai, R., Sarcoplasmic reticulum genes are upregulated in mild cardiac hypertrophy but downregulated in severe cardiac hypertrophy induced by pressure overload (1996) J Mol Cell Cardiol, 28, pp. 1583-1590 Bassani, J.W.M., Bassani, R.A., Bers, D.M., Twitch-dependent SR Ca accumulation and release in rabbit ventricular myocytes (1993) Am J Physiol Cell Physiol, 265, pp. C533-C540 Bassani, J.W.M., Bassani, R.A., Bers, D.M., Relaxation in rabbit and rat cardiac cells: Species-dependent differences in cellular mechanisms (1994) J Physiol, 476, pp. 279-293 Bassani, J.W.M., Yuan, W., Bers, D.M., Fractional SR Ca release is regulated by trigger Ca and SR Ca content in cardiac myocytes (1995) Am J Physiol Cell Physiol, 268, pp. C1313-C1319 Bassani, R.A., Bassani, J.W.M., Contribution of Ca 2+ transporters to relaxation in intact ventricular myocytes from developing rats (2002) Am J Physiol Heart Circ Physiol, 282, pp. H2406-H2413 Bassani, R.A., Bassani, J.W.M., Inhibition of the sarcoplasmic reticulum Ca 2+ pump with thapsigargin to estimate the contribution of Na +-Ca 2+ exchange to ventricular myocyte relaxation (2003) Braz J Med Biol Res, 36, pp. 1717-1723 Bassani, R.A., Bassani, J.W.M., Lipsius, S.L., Bers, D.M., Diastolic Ca efflux in atrial pacemaker cells and Ca-overloaded myocytes (1997) Am J Physiol Heart Circ Physiol, 273, pp. H886-H892 Bassani, R.A., Bers, D.M., Rate of diastolic Ca release from the sarcoplasmic reticulum of intact rabbit and rat ventricular myocytes (1995) Biophys J, 68, pp. 2015-2022 Bassani, R.A., De Moraes, S., Effect of repeated footshock on the chronotropic responsiveness of the isolated pacemaker of the rat: Role of β 2-adrenoceptors (1988) J Pharmacol Exp Ther, 246, pp. 316-321 Bassani, R.A., Shannon, T.R., Bers, D.M., Passive Ca 2+ binding in ventricular myocardium of neonatal and adult rats (1998) Cell Calcium, 23, pp. 433-442 Bénitah, J.P., Kerfant, B.G., Vassort, G., Richard, S., Gómez, A.M., Altered communication between L-type calcium channels and ryanodine receptors in heart failure (2002) Front Biosci, 7, pp. E263-E275 Berenji, K., Drazner, M.H., Rothermel, B.A., Hill, J.A., Does load-induced ventricular hypertrophy progress to systolic heart failure? (2005) Am J Physiol Heart Circ Physiol, 289, pp. H8-H16 Bers, D.M., (2001) Excitation-Contraction Coupling and Cardiac Contractile Force (2nd Ed.), , Dordrecht, The Netherlands: Kluwer Academic Bidasee, K., Dinçer, D., Besch Jr., H.R., Ryanodine receptor dysfunction in hearts of streptozotocin-induced diabetic rats (2001) Mol Pharmacol, 60, pp. 1356-1364 Brittsan, A.G., Kranias, E.G., Phospholamban and cardiac contractile function (2000) J Mol Cell Cardiol, 32, pp. 2131-2139 Chorvatova, A., Hart, G., Hussain, M., Na +/Ca 2+ exchange current (I Na/Ca) and sarcoplasmic reticulum Ca 2+ release in catecholamine-induced cardiac hypertrophy (2004) Cardiovasc Res, 61, pp. 278-287 Colomer, J.M., Mao, L., Rockman, H.A., Means, A.R., Pressure overload selectively upregulates Ca 2+/calmodulin- dependent protein kinase II in vivo (2003) Mol Endocrinol, 17, pp. 183-192 Delbridge, L.M., Bassani, J.W.M., Bers, D.M., Steady-state Ca 2+ fluxes and cytosolic Ca 2+ buffering in rabbit ventricular myocytes (1996) Am J Physiol Cell Physiol, 270, pp. C192-C199 Delbridge, L.M., Satoh, H., Yuan, W., Bassani, J.W.M., Qi, M., Ginsburg, K.S., Samarel, A.M., Bers, D.M., Cardiac myocyte volume, Ca 2+ fluxes, and sarcoplasmic reticulum loading in pressure-overload hypertrophy (1997) Am J Physiol Heart Circ Physiol, 272, pp. H2425-H2435 Del Monte, F., Lebeche, D., Guerrero, J.L., Tsuji, T., Doyer, A.A., Gwathmey, J.K., Hajjar, R.J., Abrogation of ventricular arrhythmias in a model of ischemia and reperfusion by targeting myocardial calcium cycling (2004) Proc Natl Acad Sci USA, 101, pp. 5662-5627 Dorn II, G.W., Robbins, J., Ball, N., Walsh, R.A., Myosin heavy chain regulation and myocyte contractile depression after LV hypertrophy in aortic-banded rats (1994) Am J Physiol Heart Circ Physiol, 267, pp. H400-H405 Dzimiri, N., Regulation of β-adrenoceptor signaling in cardiac function and disease (1999) Pharmacol Rev, 51, pp. 465-501 Eckardt, L., Kirchof, P., Breithardt, G., Haverkamp, W., Load-induced changes in repolarization: Evidence from experimental and clinical data (2001) Basic Res Cardiol, 96, pp. 369-380 Ganim, J.R., Luo, W., Ponniah, S., Grupp, I., Kim, H.W., Ferguson, D.G., Kadambi, V., Kranias, E.G., Mouse phospholamban gene expression during development in vivo and in vitro (1992) Circ Res, 71, pp. 1021-1030 Ginsburg, K.S., Weber, C.R., Bers, D.M., Control of maximum sarcoplasmic reticulum Ca load in intact ferret ventricular myocytes. Effects of thapsigargin and isoproterenol (1998) J Gen Physiol, 111, pp. 494-504 Hasenfuss, G., Alterations of calcium-regulatory proteins in heart failure (1998) Cardiovasc Res, 37, pp. 279-289 Ito, K., Yan, X., Tajima, M., Su, Z., Barry, W.H., Lorell, B.H., Contractile reserve and intracellular calcium regulation in mouse myocytes from normal and hypertrophied failing hearts (2000) Circ Res, 87, pp. 588-595 Janczewski, A.M., Zahid, M., Lemster, B.H., Frye, C.S., Gibson, G., Higuchi, Y., Kranias, E.G., McTiernan, C.F., Phospholamban gene ablation improves calcium transients but not cardiac function in a heart failure model (2004) Cardiovasc Res, 62, pp. 468-480 Kim, D.H., Mkaparu, F., Kim, C., Caroll, R.F., Alterations of Ca 2+ release channel function in sarcoplasmic reticulum of pressure-overloadinduced hypertrophic rat heart (1994) J Mol Cell Cardiol, 26, pp. 1505-1512 Linck, B., Bokník, P., Eschenhagen, T., Müller, F.U., Neumann, J., Nose, M., Jones, L.R., Scholz, H., Messenger RNA expression and immunological quantification of phospholamban and SR-Ca 2+-ATPase in failing and nonfailing human heart (1996) Cardiovasc Res, 31, pp. 625-632 Maier, L.S., Bers, D.M., Calcium, calmodulin and calcium-calmodulin kinase II: Heartbeart to heartbeat and beyond (2002) J Mol Cell Cardiol, 34, pp. 919-939 Maier, L.S., Brandes, R., Pieske, B., Bers, D.M., Effects of left ventricular hypertrophy on force and Ca 2+ handling in isolated rat myocardium (1998) Am J Physiol Heart Circ Physiol, 274, pp. H1361-H1370 McCall, E., Ginsburg, S., Bassani, R.A., Shannon, T.R., Qi, M., Samarel, A.M., Bers, D.M., Ca flux, contractility, and excitation-contraction coupling in hypertrophic rat ventricular myocytes (1998) Am J Physiol Heart Circ Physiol, 274, pp. H1348-H1360 Mészaros, J., Khananshvili, D., Hart, G., Mechanisms underlying delayed afterdepolarizations in hypertrophied left ventricle myocytes of rats (2001) Am J Physiol Heart Circ Physiol, 281, pp. H903-H914 Minamisawa, S., Hoshijima, M., Chu, G., Ward, C.A., Frank, K., Gu, Y., Martone, M.E., Chien, K.R., Chronic phospholamban-sarcoplasmic reticulum interaction is the critical calcium cycling defect in dilated cardiomyopathy (1999) Cell, 99, pp. 313-322 Miyamoto, M., Del Monte, F., Schmidt, U., Disalvo, T., Kang, Z.B., Matsui, T., Guerrero, J.L., Hajjar, R.J., Adenoviral gene transfer of SERCA2a improves left-ventricular function in aortic-banded rats in transition to heart failure (2000) Proc Natl Acad Sci USA, 97, pp. 793-798 Nadruz Jr., W., Kobarg, C.B., Constancio, S.S., Corat, P.D.C., Franchini, K.G., Load-induced transcription activation of c-jun in rat myocardium. Regulation by myocyte enhancer factor 2 (2003) Circ Res, 92, pp. 243-251 Nakamura, A., Rokosh, D.G., Paccanaro, M., Yee, R.R., Simpson, P.C., Grossman, W., Foster, E., LV systolic performance improves with development of hypertrophy after transverse aortic constriction in mice (2001) Am J Physiol Heart Circ Physiol, 281, pp. H1104-H1112 Nediani, C., Formigli, L., Perna, A.M., Ibba-Manneschi, L., Zecchi-Orlandini, S., Fiorillo, C., Ponziani, V., Nassi, P., Early changes induced in the left ventricle by pressure overload: An experimental study on swine heart (2000) J Mol Cell Cardiol, 32, pp. 131-142 Ohkusa, T., Hisamatsu, Y., Yano, M., Kobayashi, S., Tatsuno, H., Saiki, Y., Kohno, M., Matsuzaki, M., Altered cardiac mechanism and sarcoplasmic reticulum function in pressure overload-induced cardiac hypertrophy in rats (1997) J Mol Cell Cardiol, 29, pp. 45-54 Piacentino III, V., Weber, C.R., Chen, X., Weisser-Thomas, J., Margulies, K.B., Bers, D.M., Houser, S.R., Cellular basis of abnormal calcium transients of failing human ventricular myocytes (2003) Circ Res, 92, pp. 651-658 Pogwizd, S.M., Schlotthauer, K., Li, L., Yuan, W., Bers, D.M., Arrhythmogenesis and contractile dysfunction in heart failure. Roles of sodiumcalcium exchange, inward rectifier potassium channels and residual β-adrenergic responsiveness (2001) Circ Res, 88, pp. 1159-1167 Puglisi, J.L., Bassani, R.A., Bassani, J.W.M., Amin, J.N., Bers, D.M., Temperature and relative contributions of Ca transport systems in cardiac myocyte relaxation (1996) Am J Physiol Heart Circ Physiol, 270, pp. H1772-H1778 Qi, M., Shannon, T.R., Euler, D.E., Bers, D.M., Samarel, A.M., Down-regulation of sarcoplasmic reticulum Ca 2+-ATPase during progression of left ventricular hypertrophy (1997) Am J Physiol Heart Circ Physiol, 272, pp. H2416-H2424 Reinecke, H., Vetter, R., Drexler, H., Effects of α-adrenergic stimulation on the sarcolemmal Na +-Ca 2+-exchanger in adult rat ventricular cardiomyocytes (1997) Cardiovasc Res, 36, pp. 16-22 Rodriguez, M.M., Chen, C.H., Smith, B.L., Mochly-Rosen, D., Characterization of the binding and phosphorylation of cardiac calsequestrin by ε protein kinase C (1999) FEBS Lett, 454, pp. 240-246 Schultz, J.E.J., Glascock, B.J., Witt, S.A., Nieman, M.L., Nattamai, K.J., Liu, L.H., Lorenz, J.N., Periasamy, M., Accelerated onset of heart failure in mice during pressure overload with chronically decreased SERCA2 calcium pump activity (2004) Am J Physiol Heart Circ Physiol, 286, pp. H1146-H1153 Scopacasa, B.S., Teixeira, V.P.A., Franchini, K.G., Colchicine attenuates left ventricular hypertrophy but preserves cardiac function of aorticconstricted rats (2003) J Appl Physiol, 94, pp. 1627-1633 Shannon, T.R., Ginsburg, K.S., Bers, D.M., Potentiation of fractional SR Ca release by total and free intra-SR Ca concentration (2000) Biophys J, 78, pp. 334-343 Shannon, T.R., Ginsburg, K.S., Bers, D.M., Quantitative assessment of the SR Ca 2+ leak-load relationship (2002) Circ Res, 91, pp. 594-600 Shorofsky, S.R., Aggarwal, R., Corretti, M., Baffa, J.M., Strum, J.M., Al-Seikhan, B.A., Kobayashi, Y.M., Balke, C.W., Cellular mechanisms of altered contractility in the hypertrophied heart. Big hearts, big sparks (1999) Circ Res, 84, pp. 424-434 Sipido, K.R., Volders, P.G.A., Groot, S.H.M., Verdonck, F., Werf, F., Wellens, H.J., Vos, M.A., Enhanced Ca 2+ release and Na/Ca exchange activity in hypertrophied canine ventricular myocytes. Potential link between contractile adaptation and arrhythmogenesis (2000) Circulation, 102, pp. 2137-2144 Siri, F.M., Sympathetic changes during development of cardiac hypertrophy in aortic-constricted rats (1988) Am J Physiol Heart Circ Physiol, 255, pp. H425-H427 Szymanska, G., Strömer, H., Kim, D.H., Lorell, B.H., Morgan, J.P., Dynamic changes in sarcoplasmic reticulum function in cardiac hypertrophy and failure (2000) Pflügers Arch, 439, pp. 339-348 Tagawa, H., Wang, N., Narishige, T., Ingber, D.E., Zile, M.R., Cooper, I.V.G., Cytoskeletal mechanics in pressure-overload cardiac hypertrophy (1997) Circ Res, 80, pp. 281-289 Torsoni, A.S., Fonseca, P.M., Crosara-Alberto, D.P., Franchini, K.G., Early activation of p160 ROCK by pressure overload in rat heart (2003) Am J Physiol Cell Physiol, 284, pp. C1411-C1419 Trafford, A.W., Díaz, M.E., O'Neill, S.C., Eisner, D.A., Integrative analysis of calcium signaling in cardiac muscle (2002) Front Biosci, 1, pp. D843-D852 Wehrens, X.H.T., Lehnart, S.E., Marks, A.R., Intracellular calcium release and cardiac disease (2005) Annu Rev Physiol, 67, pp. 69-98 Zhang, T., Miyamoto, S., Brown, J.H., Cardiomyocyte calcium and calcium/calmodulin-dependent protein kinase II: Friends or foes? (2004) Recent Prog Horm Res, 59, pp. 141-168