Artículos de revistas
Development And Characterization Of Microsatellite Loci For Tabebuia Cassinoides (bignoniaceae)
Registro en:
Genetics And Molecular Research. Fundacao De Pesquisas Cientificas De Ribeirao Preto, v. 13, n. 3, p. 5601 - 5605, 2014.
16765680
10.4238/2014.July.25.15
2-s2.0-84904979081
Autor
Pretti V.Q.
Francoso E.
Sforca D.A.
Pinheiro F.
Meyer D.
Lohmann L.G.
Institución
Resumen
Tabebuia cassinoides (Lam.) DC., popularly known as caxeta, is a tree species that belongs to the plant family Bignoniaceae. This species is endemic to the Brazilian Atlantic Forest and is widely exploited commercially. To date, little is known about its genetic structure, preventing the establishment of adequate management plans for this taxon. The objective of this study was to construct a microsatellite-enriched genomic library for T. cassinoides to select polymorphic loci, and standardize polymerase chain reaction amplification conditions. Of the 15 loci examined, 5 were polymorphic. The number of alleles per locus ranged from 2 to 8, with a mean of 4.4. The microsatellite loci described here represent the basis for detailed population genetic studies of this species, which will greatly contribute for the development of better conservation strategies for this taxon. © FUNPEC-RP. 13 3 5601 5605 Billotte, N., Lagoda, P.J.R., Risterucci, A.M., Baurens, F.C., Microsatellite-enriched libraries: Applied methodology for the development of SSR markers in tropical crops (1999) Fruits, 54, pp. 277-288 Braga, A.C., Reis, A.M.M., Leoi, L.T., Pereira, R.W., Development and characterization of microsatellite markers for the tropical tree species Tabebuia aurea (Bignoniaceae) (2007) Mol. Ecol. Notes, 7, pp. 53-56 Collevatti, R.G., de Castro, T.G., de Souza, L.J., de Campos, T.M.P., Phylogeography of Tibouchina papyrus (Pohl) Toledo (Melastomataceae), an endangered tree species from rocky savannas, suggests bidirectional expansion due to climate cooling in the Pleistocene (2012) Ecol. Evol, 2, pp. 1024-1035 Diniz-Filho, J.A.F., Melo, D.B., Oliveira, G., Collevatti, R.G., Planning for optimal conservation of geographical genetic variability within species (2012) Conserv. Genet, 13, pp. 1085-1093 Drummond, A.J., Ashton, B., Buxton, S., Cheung, M., (2011) Geneious 5.3.5, , http://www.geneious.com/, Available at, Accessed June 18, 2013 Excoffier, L., Lischer, H.E., Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows (2010) Mol. Ecol. Resour, 10, pp. 564-567 Francisco, F.O., Brito, R.M., Santiago, L.R., Gonçalves, P.H.P., Isolation and characterization of 15 microsatellite loci in the stingless bee Plebeia remota (Apidae: Meliponini) (2011) Conserv. Genet. Resour, 3, pp. 417-419 Gentry, A.H., (1992) Flora Neotropica, Monograph 25 (II), Bignoniaceae Part II (Tribo Tecomeae, , New York Botanical Garden Press, New York Kijas, J.M., Fowler, J.C., Garbett, C.A., Thomas, M.R., Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidin-coated magnetic particles (1994) Biotechniques, 16, pp. 656-660 Mayer, C., (2010), http://www.rub.de/spezzoo/cm/cm_phobos.htm, Phobos v. 3.3.11. Available at, Accessed June 18, 2013Peakall, R., Smouse, P.E., GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research (2006) Mol. Ecol. Notes, 6, pp. 288-295 Pinheiro, F., De, B.F., Palma-Silva, C., Meyer, D., Hybridization and introgression across different ploidy levels in the Neotropical orchids Epidendrum fulgens and E. Puniceoluteum (Orchidaceae) (2010) Mol. Ecol, 19, pp. 3981-3994 Pinheiro, F., Barros, F., Palma-Silva, C., Fay, M.F., Phylogeography and genetic differentiation along the distributional range of the orchid Epidendrum fulgens: A Neotropical coastal species not restricted to glacial refugia (2011) J. Biogeogr, 38, pp. 1923-1935 Pinheiro, F., Cozzolino, S., Barros, F., Gouveia, T.M.Z.M., Phylogeographic structure and outbreeding depression reveal early stages of reproductive isolation in the neotropical orchid Epidendrum denticulatum (2013) Evolution, 67, pp. 2024-2039 Rousset, F., GENEPOP'007: A complete re-implementation of the GENEPOP software for Windows and Linux (2008) Mol. Ecol. Resour, 8, pp. 103-106 Rozen, S., Skaletsky, H.J., Primer3 on the WWW for General Users and for Biologist Programmers (2000) Bioinformatics Methods and Protocols: Methods In Molecular Biology, pp. 365-386. , Krawetz S and Misener S, eds.). Humana Press, Totowa Schlötterer, C., Genome evolution: Are microsatellites really simple sequences? (1998) Curr. Biol, 8, pp. R132-R134 Schuelke, M., An economic method for the fluorescent labeling of PCR fragments (2000) Nat. Biotechnol, 18, pp. 233-234 Sunnucks, P., Efficient genetic markers for population biology (2000) Trends Ecol. Evol, 15, pp. 199-203