Intrahippocampal Infusion Of Crotamine Isolated From Crotalus Durissus Terrificus Alters Plasma And Brain Biochemical Parameters

dc.creatorGoncalves R.
dc.creatorVargas L.S.
dc.creatorLara M.V.S.
dc.creatorGullich A.
dc.creatorMandredini V.
dc.creatorPonce-Soto L.
dc.creatorMarangoni S.
dc.creatorDal Belo C.A.
dc.creatorMello-Carpes P.B.
dc.date2014
dc.date2015-06-25T17:53:44Z
dc.date2015-11-26T14:26:11Z
dc.date2015-06-25T17:53:44Z
dc.date2015-11-26T14:26:11Z
dc.date.accessioned2018-03-28T21:29:09Z
dc.date.available2018-03-28T21:29:09Z
dc.identifier
dc.identifierInternational Journal Of Environmental Research And Public Health. Mdpi Ag, v. 11, n. 11, p. 11438 - 11449, 2014.
dc.identifier16617827
dc.identifier10.3390/ijerph111111438
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84908568813&partnerID=40&md5=31928afbff166e0f2e3dc0a60d17b81b
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/86518
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/86518
dc.identifier2-s2.0-84908568813
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1246007
dc.descriptionCrotamine is one of the main constituents of the venom of the South American rattlesnake Crotalus durissus terrificus. Here we sought to investigate the inflammatory and toxicological effects induced by the intrahippocampal administration of crotamine isolated from Crotalus whole venom. Adult rats received an intrahippocampal infusion of crotamine or vehicle and were euthanized 24 h or 21 days after infusion. Plasma and brain tissue were collected for biochemical analysis. Complete blood count, creatinine, urea, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), creatine-kinase (CK), creatine kinase-muscle B (CK-MB) and oxidative parameters (assessed by DNA damage and micronucleus frequency in leukocytes, lipid peroxidation and protein carbonyls in plasma and brain) were quantified. Unpaired and paired t-tests were used for comparisons between saline and crotamine groups, and within groups (24 h vs. 21 days), respectively. After 24 h crotamine infusion promoted an increase of urea, GOT, GPT, CK, and platelets values (p ≤ 0.01), while red blood cells, hematocrit and leukocytes values decreased (p ≤ 0.01). Additionally, 21 days after infusion crotamine group showed increased creatinine, leukocytes, TBARS (plasma and brain), carbonyl (plasma and brain) and micronucleus compared to the saline-group (p ≤ 0.01). Our findings show that crotamine infusion alter hematological parameters and cardiac markers, as well as oxidative parameters, not only in the brain, but also in the blood, indicating a systemic pro-inflammatory and toxicological activity. A further scientific attempt in terms of preserving the beneficial activity over toxicity is required.
dc.description11
dc.description11
dc.description11438
dc.description11449
dc.descriptionTribuiani, N., da Silva, A.M., Ferraz, M.C., Silva, M.G., Bentes, A.P.G., Graziano, T.S., dos Santos, M.G., Groppo, F.C., Vellozia flavicans Mart. ex Schult. hydroalcoholic extract inhibits the neuromuscular blockade induced by Bothrops jararacussu venom (2014) BMC Complement. Altern. Med., 14
dc.descriptionJorge, M.T., Ribeiro, L.A., Epidemiologia e quadro clínico do acidente por cascavel sul-americana (Crotalus durissus) (1992) Rev. Inst. Med. Trop. São Paulo, 34, pp. 347-354
dc.descriptionKerkis, I., de Sá Silva, F., Pereira, A., Kerkis, A., Rádis-Baptista, G., Biological versatility of crotamine-A cationic peptide from the venom of a South American rattlesnake (2010) Expert Opin. Investig. Drugs, 19, pp. 1515-1525
dc.descriptionHernandez-Oliveira e Silva, S., Rostelato-Ferreira, S., Rocha-E-Silva, T.A.A., Randazzo-Moura, P., Dal-Belo, C.A., Sanchez, E.F., Borja-Oliveira, C.R., Rodrigues-Simioni, L., Beneficial effect of crotamine in the treatment of myasthenic rats (2013) Muscle Nerve, 47, pp. 591-593
dc.descriptionVargas, L.S., Lara, M.V., Gonçalves, R., Mandredini, V., Ponce-Soto, L.A., Marangoni, S., Dal-Belo, C.A., Mello-Carpes, P.B., The intrahippocampal infusion of crotamine from Crotalus durissus terrificus venom enhances memory persistence in rats (2014) Toxicon, 85, pp. 52-58
dc.descriptionMancin, A.C., Soares, A.M., Andrião-Escarso, S.H., Faça, V.M., Greene, L.J., Zuccolotto, S., Pelá, I.R., Giglio, J.R., The analgesic activity of crotamine, a neurotoxin from Crotalus durissus terrificus (South American rattlesnake) venom: A biochemical and pharmacological study (1998) Toxicon, 36, pp. 1927-1937
dc.descriptionRuff, R.L., Lennon, V.A., End-plate voltage-gated sodium channels are lost in clinical and experimental myasthenia gravis (1998) Ann. Neurol, 43, pp. 370-379
dc.descriptionRizzi, C.T., Carvalho-De-Souza, J.L., Schiavon, E., Cassola, A.C., Wanke, E., Troncone, L.R.P., Crotamine inhibits preferentially fast-twitching muscles but is inactive on sodium channels (2007) Toxicon, 50, pp. 553-562
dc.descriptionCamillo, M.A.P., Arruda Paes, P.C., Troncone, L.R.P., Rogero, J.R., Gyroxin fails to modify in vitro release of labelled dopamine and acetylcholine from rat and mouse striatal tissue (2001) Toxicon, 39, pp. 843-853
dc.descriptionRobinson, L., Platt, B., Riedel, G., Involvement of the cholinergic system in conditioning and perceptual memory (2011) Behav. Brain Res, 221, pp. 443-465
dc.descriptionRossato, J.I., Radiske, A., Kohler, C.A., Gonzalez, C., Bevilaqua, L.R., Medina, J.H., Cammarota, M., Consolidation of object recognition memory requires simultaneous activation of dopamine D1/D5 receptors in the amygdala and medial prefrontal cortex but not in the hippocampus (2013) Neurobiol. Learn. Mem., 106, pp. 66-70
dc.descriptionBerrih-Aknin, S., Le Panse, R., Myasthenia gravis: A comprehensive review of immune dysregulation and etiological mechanisms (2014) J. Autoimmun, 52, pp. 90-100
dc.descriptionDal-Belo, C.A., de Bairros Lucho, A.P., Vinadé, L., Rocha, L., Seibert França, H., Marangoni, S., Rodrigues-Simioni, L., In vitro antiophidian mechanisms of Hypericum brasiliense choisy standardized extract: Quercetin dependent neuroprotection (2013) Biomed. Res. Int., 2013
dc.descriptionHabermann, E., Cheng-Raude, D., Central neurotoxicity of apamin, crotamin, phospholipase A and alpha-amanitin (1975) Toxicon, 13, pp. 465-473
dc.descriptionMello, L.E.A.M., Cavalheiro, E.A., Behavioural, electroencephalographic and neuropathological effects of the intrahippocampal injection of the venom of the South American rattlesnake (Crotalus durissus terrificus) (1989) Toxicon, 27, pp. 189-199
dc.descriptionPaxinos, G., Watson, C., (1986) The Rat Brain in Stereotaxic Coordinates, , Academic Press: San Diego, CA, USA
dc.descriptionSingh, N.P., McCoy, M.T., Tice, R.R., Schneider, E.L., A simple technique for quantitation of low levels of DNA damage in individual cells (1988) Exp. Cell Res., 175, pp. 184-191
dc.descriptionOhkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal. Biochem., 95, pp. 351-358
dc.descriptionLevine, R.L., Garland, D., Oliver, C.N., Amici, A., Climent, I., Lenz, A.G., Ahn, B.W., Stadtman, E.R., Determination of carbonyl content in oxidatively modified proteins (1990) Methods Enzymol., 186, pp. 464-478
dc.descriptionPinho, F.M.O., Yu, L., Burdmann, E.A., Snakebite-induced kidney injury in Latin America (2008) Semin. Nephrol, 28, pp. 354-362
dc.description(1991) Ofidismo: Análise Epidemiológica, , Brasil. Ministério da Saúde: Brasília, Brazil
dc.descriptionAmaral, C.F.S., Rezende, N.A., da Silva, O.A., Ribeiro, M.M.F., Magalhães, R.A., Reis, R.J.D., Carneiro, J.G., Castro, J.R.S., Insuficiência renal aguda secundária a acidentes ofídicos botrópico e crotálico: Análise de 63 casos (1896) Rev. Inst. Med. Trop. São Paulo, 28, pp. 220-227
dc.descriptionLomonte, B., Angulo, Y., Calderón, L., An overview of lysine-49 phospholipase A2 myotoxins from crotalid snake venoms and their structural determinants of myotoxic action (2003) Toxicon, 42, pp. 885-901
dc.descriptionMontecucco, C., Gutiérrez, J.M., Lomonte, B., Cellular pathology induced by snake venom phospholipase A2 myotoxins and neurotoxins: Common aspects of their mechanisms of action (2008) Cell. Mol. Life Sci., 65, pp. 2897-2912
dc.descriptionOguiura, N., Boni-Mitake, M., Radis-Baptista, G., New view on crotamine, a small basic polypeptide myotoxin from South American rattlesnake venom (2005) Toxicon, 46, pp. 363-370
dc.descriptionPeigneur, S., Orts, D.J.B., da Silva, A.R.P., Oguiura, N., Boni-Mitake, M., de Oliveira, E.B., Zaharenko, A.J., Tytgat, J., Crotamine pharmacology revisited: Novel insights based on the inhibition of KV channels (2012) Mol. Pharmacol., 82, pp. 90-96
dc.descriptionDal-Belo, C.A., Leite, G.B., Toyama, M.H., Marangoni, S., Corrado, A.P., Fontana, M.D., Souhan, A., Rodrigues-Simioni, L., Pharmacological and structural characterization of a novel phospholipase A2 from Micrurus dumerilii carinicauda venom (2005) Toxicon, 46, pp. 736-750
dc.descriptionDe Sousa-E-Silva, M.C., Tomy, S.C., Tavares, F.L., Navajas, L., Larsson, M.H., Lucas, S.R., Kogika, M.M., Sano-Martins, I.S., Hematological, hemostatical and clinical chemistry disturbances induced by Crotalus durissus terrificus sanke venom in dogs (2003) Hum. Exp. Toxicol., 22, pp. 491-500
dc.descriptionSebastin Santhosh, M., Thushara, R.M., Hemshekhar, M., Sunitha, K., Devaraja, S., Kemparaju, K., Girish, K.S., Allevation of viper venom induced platelet apoptosis by crocin (Crocus sativus): Implication for thrombocytopenia in viper bites (2013) J. Thromb. Thrombolsys, 36, pp. 424-432
dc.descriptionWaghmare, A.B., Salvi, N.C., Deopurkar, R.L., Shenoy, P.A., Sonpetkar, J.M., Evaluation of health status of horses immunized with snake venom and montanide adjuvants, IMS 3012 (nanoparticle), ISA 206 and ISA 35 (emulsion based) during polyvalent snake antivenom production: Hematological and biochemical assessment (2014) Toxicon, 82, pp. 83-92
dc.descriptionLin, J.D., Lin, P.Y., Chen, L.M., Fang, W.H., Lin, L.P., Loh, C.H., Serum glutamic-oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels in children and adolescents with intellectual disabilities (2010) Res. Dev. Disabil, 31, pp. 172-177
dc.descriptionAl-Sadoon, M.K., Fahim, A., Possible recovery from an acute envenomation in male rats with LD50 of Echis coloratus crude venom: I-A seven days hematological follow-up study (2012) Saudi J. Biol. Sci., 19, pp. 221-227
dc.descriptionWang, X., Michaelis, E.K., Selective neuronal vulnerability to oxidative stress in brain (2010) Front. Aging Neurosci, 2
dc.descriptionDa Silva, J.G., da Silva Soley, B., Gris, V., do Rocio Andrade Pires, A., Caderia, S.M.S.C., Eler, G.J., Hermoso, A.P.M., Acco, A., Effects of the Crotalus durissus terrificus snake venom on hepatic metabolism and oxidative stress (2011) J. Biochem. Mol. Toxicol, 25, pp. 195-203
dc.descriptionNiki, E., Lipid peroxidation: Physiological levels and dual biological effects (2009) Free Radic. Biol. Med, 47, pp. 469-484
dc.descriptionCatalá, A., Lipid peroxidation of membrane phospholipids generate hidroxy-alkenals and oxidized phospholipids active in physiological and/or pathological conditions (2009) Chem. Phys. Lipids, 157, pp. 1-11
dc.descriptionPereira, A., Kerkis, A., Hayashi, M.A.F., Pereira, A.S.P., Silva, F.S., Oliveira, E.B., da Silva, A.R.B.P., Kerkis, I., Crotamine toxicity and efficacy in mouse models of melanoma (2011) Expert. Opin. Investig. Drugs, 20, pp. 1189-1200
dc.descriptionBoni-Mitake, M., Costa, H., Spencer, P.J., Vassilieff, V.S., Rogero, J.R., Effects of 60Co gamma radiation on crotamine (2001) Braz. J. Med. Biol. Res., 34, pp. 1531-1538
dc.descriptionBoni-Mitake, M., Costa, H., Vassilieff, V.S., Rogero, J.R., Distribution of 125I-labeled crotamine in mice tissues (2006) Toxicon, 48, pp. 550-555
dc.languageen
dc.publisherMDPI AG
dc.relationInternational Journal of Environmental Research and Public Health
dc.rightsaberto
dc.sourceScopus
dc.titleIntrahippocampal Infusion Of Crotamine Isolated From Crotalus Durissus Terrificus Alters Plasma And Brain Biochemical Parameters
dc.typeArtículos de revistas


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