Actas de congresos
Enantioseparation Of Secnidazole By High-performance Liquid Chromatography Using Amylose-based Stationary Phase
Registro en:
Adsorption Science And Technology. , v. 30, n. 8-9, p. 739 - 750, 2012.
2636174
10.1260/0263-6174.30.8-9.739
2-s2.0-84879547890
Autor
Nascimento A.C.
Perna R.F.
Cremasco M.A.
Santana C.C.
Institución
Resumen
The secnidazole, a drug used in hepatic amebiasis, giardiasis and bacterial vaginosis, was enantioseparated on a semi-preparative scale by high performance liquid chromatography (HPLC), using chiral stationary phase (CSP) containing amylose tris (3, 5-dimethylphenyl) carbamate on silica gel (Chiralpak® AD). The experiments were carried out under normal phase conditions and the separation method exhibited very high selectivity and resolutions. The chromatographic parameters and the effects of column temperature on the retention time and selectivity were evaluated. Thermodynamic parameters were obtained from van't Hoff plots, and the elution order of the enantiomers was determined by circular dichorism. The enantiomers of secnidazole were separated under overloaded conditions with a good performance. 30 8-9 739 750 Aboul-Enein, H.Y., Ali, I., (2001) Fresenius' J. Anal. Chem., 370, p. 951 Ali, I., Aboul-Enein, H.Y., Gaitonde, V.D., Singh, P., Rawat, M.S.M., Sharma, B., (2009) Chromatographia, 70, p. 223 Andersson, S., Alenmark, S.G.C., (2002) J. Biochem. Biophys. Methods, 54, p. 11 Backer, E., Dubreuil, L., Brauman, M., Acar, J., Vaneechoutte, M., (2010) Clin. Microbiol. Infect., 16, p. 470 Bakshi, M., Singh, S., (2004) J. Pharm. Biomed. Anal., 36, p. 769 Blanco, M., Coelho, J., Iturriaga, H., Maspoch, S., Porcel, M., (2001) Anal. Chim. Acta, 431, p. 115 Cane, H., Groner, E., Levy, L., Agranat, I., (2004) Drug Discovery Today, 9, p. 105 Collins, C.H., Braga, G.L., Bonato, P.S., (2006) Fundamentals of Chromatography, , Unicamp, São Paulo, Brazil El Walily, A.F.M., Abdine, H.H., Razak, O.A., Zamel, S., (2000) J. Pharm. Biomed. Anal., 22, p. 887 Fornstedt, T., Sajonz, P., Guiochon, G., (1997) J. Am. Chem. Soc., 119, p. 1254 Gillis, J.C., Wiseman, L.R., (1996) J. Drug Eval., 51, p. 621 Macherey-Nagel (2001) Guide to Chromatography Columns, , Düren, Germany Núñez, J.T., Gomez, G., (2005) Int. J. Gynecol. Obstet., 88, p. 281 Raether, W., Hänel, H., (2003) Parasitol. Res., 90, pp. S19 Schulte, M., Epping, A., (2005) Preparative Chromatography of Fine Chemicals and Pharmaceutical Agents, , Wiley-VCH, Weinheim, Germany Shen, J., Ikai, T., Okamoto, Y., (2010) J. Chromatogr. A, 1217, p. 1041 Seidel-Morgenstern, A., (2004) J. Chromatogr. A, 1037, p. 255 Silva Jr., I.J., Sartor, J.P., Rosa, P.C.P., Veredas, V., Barreto Jr., A.G., Santana, C.C., (2007) J. Chromatogr. A, 1162, p. 97 Stern, E., Goossens, L., Vaccher, C., Bonte, J.P., Depreux, P., Henichart, J.P., Goossens, J.F., (2008) J. Pharm. Biomed. Anal., 46, p. 848 Weng, W., Guo, H., Zhan, F., Fang, H., Wang, Q., Yao, B., Li, S., (2008) J. Chromatogr. A, 1210, p. 178 Weng, W., Wang, Q.H., Yao, B.X., Zeng, Q.L., (2004) J. Chromatogr. A, 1042, p. 81 Yamamoto, C., Hayashi, T., Okamoto, Y., (2003) J. Chromatogr. A, 1021, p. 83