Determination Of Crystal Growth Rate For Porcine Insulin Crystallization With Co 2 As A Volatile Acidifying Agent

dc.creatorHirata G.A.M.
dc.creatorBernardo A.
dc.creatorMiranda E.A.
dc.date2012
dc.date2015-06-26T20:29:29Z
dc.date2015-11-26T14:26:02Z
dc.date2015-06-26T20:29:29Z
dc.date2015-11-26T14:26:02Z
dc.date.accessioned2018-03-28T21:28:54Z
dc.date.available2018-03-28T21:28:54Z
dc.identifier
dc.identifierChemical Engineering And Processing: Process Intensification. , v. 56, n. , p. 29 - 33, 2012.
dc.identifier2552701
dc.identifier10.1016/j.cep.2012.03.001
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84860886633&partnerID=40&md5=58bd98cc53f056614936588412670b37
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/97050
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/97050
dc.identifier2-s2.0-84860886633
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1245949
dc.descriptionCrystallization is controlled by two steps that determine the quality and the final size of the product, nucleation and growth, which are functions of supersaturation. Recently, Hirata et al. [1] crystallized insulin using CO 2 as a volatile acid to impose supersaturation on the system. The objective of the present work was to determine the growth kinetics of insulin crystallization in 50mM NaHCO 3 solution with 0.4mM ZnCl 2 in a CO 2 atmosphere at 15°C, adjusting the parameters of the equation G=k g×S g to the experimental data. The solubility of insulin in the NaHCO 3/CO 2/ZnCl 2 system at 15°C was determined as a function of pH in the range of 6.30-7.34. The crystal growth data allowed determination of the growth order " g" (g=2.9). Although protein crystallization has some features that differ from the crystallization of less complex molecules, the apparent growth kinetics of insulin were successfully analyzed here with the same empirical methods used for small molecules, which can easily be scaled up for industrial applications to achieve specific size and purity, the goals of industrial crystallization. The method used in this work is a useful tool for describing and simplifying optimization of industrial protein crystallization processes. © 2012 Elsevier B.V.
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dc.languageen
dc.publisher
dc.relationChemical Engineering and Processing: Process Intensification
dc.rightsfechado
dc.sourceScopus
dc.titleDetermination Of Crystal Growth Rate For Porcine Insulin Crystallization With Co 2 As A Volatile Acidifying Agent
dc.typeArtículos de revistas


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