Artículos de revistas
The Effects Of Acute Exercise On Serum Biomarkers In Diabetics Rats [efeitos Do Exercício Agudo Sobre Biomarcadores Séricos De Ratos Diabéticos]
Registro en:
Revista Brasileira De Medicina Do Esporte. Redprint Editora Ltda, v. 20, n. 1, p. 32 - 35, 2014.
15178692
10.1590/S1517-86922014000100006
2-s2.0-84896905267
Autor
Milioni F.
Antunes B.M.M.
Teixeira-Arroyo C.
Loures J.P.
Scariot P.P.M.
dos Santos P.C.R.
Silva M.S.
Luciano E.
Institución
Resumen
Introduction: The responses to acute exercise on biomarkers, such as alkaline phosphatase (ALP) and creatine kinase (CK) serum levels have been little investigated in diabetic rats. Objectives: To investigate the effects of acute aerobic exercise on the concentrations of CK and ALP as well as evaluating the hydration status in diabetic rats. Materials and methods: Were used male Wistar rats, young adults, divided into two groups: diabetic (DA) and controls (CA). The diabetes was induced in the rats by administration of alloxan monohydrate Sigma® (32 mg/kg body weight). Two weeks after confirmation of diabetes, both groups were subjected to an acute swim session for 30 min, with aerobic load (4.5% body weight). Glucose, hematocrit, CK, ALP, albumin and lactate kinetics during exercise were evaluated by collecting 25μL of blood from the tail of the animals in minutes 0, 10, 20 and 30 of exercise. Results: Two-way ANOVA for repeated measures and post hoc Tukey test showed significant decrease of glycemia after exercise for the DA group, significant increase in CK after exercise for the DA group, significant increase in hematocrit for both groups after exercise and maintenance of ALP after exercise for the DA group. Conclusion: The acute aerobic exercise was effective in controlling glucose levels in diabetic rats. However, it should be applied with caution, because it induced high CK values, suggesting possible tissue damage. 20 1 32 35 Araujo, G.G., Papoti, M., Dos Reis, I.G.M., De Mello, M.A.R., Gobatto, C.A., Physiological responses during linear periodized training in rats (2011) Eur J Appl Physiol, 112 (3), pp. 839-852 Gobatto, C.A., Mello, M.A.R., Sibuya, C.Y., Azevedo, J.R.M., Santos, L., Kokubun, E., Maximal lactate steady state in rats submitted to swimming exercise (2001) Comparative Biochemistry and Physiology, 130, pp. 21-27. , Parte A Luciano, E., Lima, F.B., Metabolismo de ratos diabéticos treinados submetidos ao jejum e ao exercício agudo (1997) Revista De Ciências Biomédicas, 18, pp. 47-60 Oliveira, C.A.M., Luciano, E., Marcondes, M.C.C.G., Mello, M.A.R., Effects of swimming training at the intensity equivalent to aerobic/anaerobic metabolic transition in alloxan diabetic rats (2007) Journal of Diabetes and Its Complications, 21, pp. 258-264 Ribeiro, C., Cambri, L.T., Dalia, R.A., Araújo, M.B., Ghezzi, A.C., Moura, L.P., Muscle protein metabolism in neonatal alloxanadministered rats: Effects of continuous and intermittent swimming training (2012) Diabetol-ogy & Metabolic Syndrome, 4, p. 5 Snowling, N.J., Hopkins, W.G., Effects of Different Modes of ExerciseTraining on Glucose Control and Risk Factors for Complications in Type 2 Diabetic Patients (2006) Diabetes Care, 29, pp. 2518-2527 Santana, R.B., Xu, L., Chase, H.B., Amar, S., Graves, D.T., Trackman, P.C., A role for advanced glycation end products in diminished bone healing in type 1 diabetes (2003) Diabetes, 52, pp. 1502-1510 Hofbauer, L.C., Brueck, C.C., Singh, S.K., Dobnig, H., Osteoporosis in Patients With Diabetes Mellitus (2007) Journal of Bone and Mineral Research, 22, pp. 1317-1328 Miazgowski, T., Czekalski, S., A 2-years Follow-up Study on Bone Mineral and Markers of Bone Turnover in Patients with Long-standing Insulin-Dependent Diabetes Mellitus (1998) Osteoporosis International, 8, pp. 399-403 Guerino, M.R., Santi, F.P., Silveira, R.F., Luciano, E., Influence of ultrasound and physical activity on bone healing (2008) Ultrasound In Medicine & Biology, 34 (9), pp. 1408-1413 Scott, F.W., Trick, K.D., Lee, L.P.K., Hynie, I., Heick, H.M.C., Nera, E.A., Serum enzymes in the BB rat before and after onset of the overt diabetic syndrome (1984) Clinical Biochemistry, 17, pp. 270-275 Brancaccio, P., Maffulli, N., Limongelli, F.M., Creatine kinase monitoring in sport medicine (2007) British Medical Bulletin, 81-82 (1), pp. 209-230 Schneider, C.M., Dennehy, C.A., Rodearmel, S.J., Hayward, J.R., Effects of physical activity on creatine phos-phokinase and the isoenzyme creatine kinase-MB (1995) Ann Ernerg Med, 25, pp. 520-524 Hevener, A.L., Reichart, D., Olefsky, J., Exercise and thiazolidinedione therapy normalize insulin action in the obese Zucker fatty rat (2000) Diabetes, 49, pp. 2154-2159 Henriksen, E.J., Invited Review: Effects of acute exercise and exercise training on insulin resistance (2002) J Appl Physiol, 93, pp. 788-796 Gao, J., Sherman, W.M., McCune, S.A., Osei, K., Effects of acute running exercise on whole body insulin action in obese male SHHF/Mcc-facp rats (1994) J Appl Physiol, 77, pp. 534-541 Araujo, G.G., Papoti, M., Manchado, F.D.B., Mello, M.A.R., Gobatto, C.A., Protocols for hyperlactatemia induction in the lactate minimun test adapted to swimming rats (2007) Comparative Biochemistry and Physiology Parte A, 148, pp. 888-892 Engels, R.C., Jones, J.B., Causes and elimination of erratic blanc in enzymatic metabolic assays involving the use of NAD in alkaline hydrazine buffers: Improved conditions for assay of L-glutamate, L-lactate and other metabolites (1978) Anal Biochem, 88, pp. 475-484 Henry, R.J., Cannon, D.C., Wilkeman, J., (1974) Clinical Chemistry, Principles and Techniques, p. 1288. , New York: Harper and Harper Row Publishes Gomes, R.J., Leme, J.A.C.A., Mello, M.A.R., Luciano, E., Caetano, F.H., Efeitos do treinamento de natação em aspectos metabólicos e morfológicos de ratos diabéticos (2008) Motriz, 14, pp. 320-328 Contarteze, R.V.L., Manchado, F.D.B., Gobatto, C.A., de Mello, M.A.R., Stress biomarkers in rats submitted to swimming and tredmill running exercise (2007) Comparative Biochemistry and Physiology. Parte A, 151, pp. 415-422 Physical activity/exercise and diabetes (Position Statement) (2004) Diabetes Care, 27, pp. S58-S62. , American Diabetes Association Rogatto, G.P., (2001) Efeitos Do Treinamento Físico De Alta Intensidade Sobre Parâmetros Endócrino-metabólicos Relacionados Ao Estresse [Dissertação De Mestrado], , Rio Claro: Universidade Estadual Paulista Lee, J., Clarkson, P.M., Plasma Creatine kinase activity and glutathione after eccentric exercise (2003) Medicine and Science In Sports and Exercise, 35, pp. 930-936 Ceretta, L.B., R'Eus, G.Z., Abelaira, H.M., Ribeiro, K.F., Zappellini, G., Felisbino, F.F., Increased oxidative stress and imbalance in antioxidant enzymes in the brains of Alloxan-induced diabetic rats (2012) Experimental Diabetes Research, pp. 1-8 Ceriello, A., New insights on oxidative stress and diabetic complications may lead to a "causal" antioxidant therapy (2003) Diabetes Care, 26, pp. 1589-1596 Pires, A.C.L., Boaventura, M.F.C., Gorjão, R., Hirabara, S.M., Puggina, E.F., Pellegrinotti, I.L., Filho, L.A.D., Cury, T.C.P., Induction of lymphocyte death by short-and-long duration triathlon competitions (2009) Medicine and Science In Sports and Exercise, 41, pp. 1896-1901 Powers, S.K., Duarte, J., Kavazis, A.N., Talbert, E.E., Reactive oxygen species are signalling molecules for skeletal muscle adaptation (2011) Exp Physiol, 95, pp. 1-9 Queiroga, M.R., Silveira, R.F., Oliveira, M.F.M., Crespilho, D., Kokubun, E., Luciano, E., Efeitos do exercício físico agu-do sobre a glicemia e lipidemia de ratos diabéticos tratados com metformina (2006) Maringá, 17, pp. 169-175 Cunningham, J.G., Termorregulação Tratado De Fisiologia Veterinária. 3 Ed, p. 2004. , Rio de Janeiro: Guanabara Koogan