Artículos de revistas
Changes In Peroxidases In The Suspension Culture Of Rubus Fruticosus During Growth
Registro en:
Plant Cell, Tissue And Organ Culture. , v. 46, n. 1, p. 27 - 33, 1996.
1676857
2-s2.0-0030477770
Autor
Cortelazzo A.L.
Marais M.-F.
Joseleau J.-P.
Institución
Resumen
The growth parameters of a cell suspension culture of Rubus fruticosus L. were determined over a culture period including exponential growth, stationary phase and a glucose starvation period at the end of the normal culture cycle. Peroxidase activities were measured in the cytoplasm, in the cell wall, and in the culture medium by the guaiacol assay. There is a relationship between the activity found in the spent medium and the dry matter mass of the cells during the exponential growth. In the three compartments a bimodal repartition of peroxidase activities was observed, with the two peaks at day 4 and day 26, respectively. This suggests that the first peak corresponds to actively dividing cells whereas the second is associated with senescence, or stress due to starvation. Fractionation of the peroxidases from the culture medium revealed the presence of two sets of cationic isoenzymes, with minor amount of anionic peroxidases. Interestingly, the second peak of cationic enzymes which was of weak intensity at day 10 of the culture, became prevalent at day 26. This indicates that not only the total amount of peroxidases varies as a function of culture time, but also that the nature of the peroxidases secreted into the medium changes during growth. 46 1 27 33 Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254 Cortelazzo, A.L., Starch detection and dosage in Canavalia ensiformis and C. gladiata cotyledons, during the plantlet development (1992) Revta. Bras. Bot., 15, pp. 157-162 Evans, J.J., Cell wall bound and soluble peroxidases in normal and dwarf tomato (1990) J.Agric. Food Chem., 38, pp. 948-951 Fry, S.C., Cross-linking of matrix polymers in the growing cell walls of the angiosperms (1981) Annu. Rev. Plant Physiol., 37, pp. 165-186 Fry, S.C., Xyloglucan: A. metabolically dynamic polysaccharide (1992) Trends Glycosci. Glycotechnol., 4, pp. 279-289 Gaspar, T.H., Penel, C., Thorpe, T., Greppin, H., (1982) Peroxidases. A Survey of Their Biochemical and Physiological Roles in Higher Plants, , Université de Genève, Genève Gaspar, T.H., Penel, C., Castillo, F.J., Greppin, H., A two-step control of basic and acidic peroxidases and its significance for growth and development (1985) Physiol. Plant., 64, pp. 418-423 Goldberg, R., Imberty, A., Liberman, M., Prat, R., Relationships between peroxidase activities and cell wall plasticity (1986) Molecular and Physiological Aspects of Plant Peroxidases, pp. 209-220. , Greppin H, Penel C & Gaspar Th (eds) Uriv. Geneva Switzerland Gotthardt, U., Grambow, H.J., Near-isogenic wheat suspension cultures: Establishment, elicitor induced peroxidase activity and potential use in the study of host/pathogen interactions (1992) J. Plant Physiol., 139, pp. 659-665 Heller, R., Recherches sur la nutrition minérale des tissus végétaux cultives in vitro (1953) Ann. Sci. Nat. Paris, 14, pp. 1-223 Hustache, G., (1983) Recherches sur la Culture In Vitro des Tissus de Rosa Glauca. Obtention d'une Suspension Cellulaire, , Thèse de doctorat, Université Scientifique et Medicale de Grenoble, Grenoble-France Joseleau, J.P., Cartier, N., Chambat, G., Faik, A., Ruel, K., Structural features and biological activity of xyloglucans from suspension-cultured plant cells (1992) Biochimie, 74, pp. 81-88 Lamport, D.T.A., Roles of peroxidases in cell wall genesis (1986) Molecular and Physiological Aspects of Plant Peroxidases, pp. 199-208. , Greppin H, Penel C & Gaspar Th (eds) Univ. Genev, Switzerland Legrand, B., Dubois, J., Evolution des peroxydases et auxines-oxydases au cours de la croissance d'une suspension cellulaire de Silène (Silene alba Miller) E.H.L. Krause (1977) C. R. Acad. Sc. Paris, 285, pp. 661-664 Li, Z.C., McClure, J.W., Hagerman, A.E., Soluble and bound apoplastic activity for peroxidase, B-D-glucosidase, malate dehydrogenase, and nonspecific arylesterase, in barley (Hordeum vulgare L,) and oat (Avena sativa) primary leaves (1989) Plant Physiol., 90, pp. 185-190 Perrey, R., Hauser, M.T., Wink, M., Cellular and subcellular localization of peroxidase isoenzymes in plants and cell suspension cultures from Lupinus polyphyllus (1989) Z. Naturforsch., 44 C, pp. 931-936 Ruel, K., Joseleau, J.P., Influence of xyloglucan oligosaccharides on the micromorphology of the walls of suspension-cultured Rubus fruticosus cells (1993) Acta Bot. Neerl., 42, pp. 363-378 Sesto, P.A., VanHuyste, R.B., Purification and yield of a cationic peroxidase from a peanut suspension cell culture (1989) Plant Sci., 61, pp. 163-168 Shedletzky, E., Shmuel, M., Delmer, D.P., Lamport, D.T.A., Adaptation and growth of tomato cells on the herbicide 2,6-dichlorobenzonitrile leads to production of unique cell walls virtually lacking a cellulose-xyloglucan network (1990) Plant Physiol., 94, pp. 980-987 Shetty, K., Bothra, D., Crawford, D.L., Korus, R.A., Extracellular peroxidases as indicators of growth in plant cell suspension cultures (1990) Appl. Biochem. Biotechnol., 24, pp. 24-35 Sherf, B.A., Kolattukudy, P.E., Developmentally regulated expression of the wound- And pathogen-responsive tomato anionic peroxidase in green fruit (1993) Plant J., 3, pp. 829-933 Sterjiades, R., Dean, J.F.D., Ericsson, K.E., Laccase from Sycamor Maple (Acer pseudoplatanus) polymerizes monolignols (1992) Plant Physiol., 99, pp. 1162-1168 Talbott, L.D., Ray, P.M., Changes in molecular size of previously deposited and newly synthesized pea cell wall matrix polysaccharides (1992) Plant Physiol., 98, pp. 369-379 Vanhuystee, R.B., Breda, C., Sesto, P., Beopouolos, N., Esnault, R., Measurement and detection of peroxidase (1990) Plant Sci., 69, pp. 19-26 Zheng, X., VanHuystee, R.B., Immunological assays on the relation of culture medium peroxidases to those in fractions isolated from peanut cells (1991) J. Plant Physiol., 138, pp. 528-532 Zheng, X., VanHuystee, R.B., Oxidation of tyrosine by peroxidase isozymes derived from peanut culture medium and by isolated cell walls (1991) Plant Cell Tiss. Org. Cult., 25, pp. 35-43 Zheng, X., VanHuystee, R.B., Peroxidase-regulated elongation of segments from peanut hypocotyls (1992) Plant Sci., 81, pp. 47-56