Artículos de revistas
Inhibition Of Microbial Metabolism By Ctab (cetyltrimethylammonium Bromide) And Its Effect On Cytoplasm Enzymes
Registro en:
Revista De Microbiologia. , v. 27, n. 3, p. 171 - 176, 1996.
13714
2-s2.0-0030462424
Autor
Ueno M.
Yokoya F.
Institución
Resumen
The effect of cetyltrimethylammonium bromide (CTAB) on the metabolism of various microorganisms was investigated. The rates of CO2 release and O2 uptake was inhibited by CTAB in a dose dependent fashion and were affected to variable degrees in the different microbial species, indicating that both respiratory and fermentative activities were inhibited. Pseudomonas aeruginosa, described as the most resistant of the tested microorganisms, was found to be 100 times less sensitive to CTAB with respect to metabolic activity. In contrast with intact cell responses, the activity of the enzyme lactate dehydrogenase extracted from sensitive and resistant species was equally inhibited by CTAB. Alcohol dehydrogenase was slightly more sensitive than lactate dehydrogenase. The concentration of CTAB required for complete inhibition of both enzymes was within the range of growth inhibition. This indicates that growth inhibition of microorganisms by CTAB could be due to its action on the metabolic activity of cytoplasm enzymes. Species resistance was related to differences in cell membrane permeability. 27 3 171 176 Anderes, E.A., Sandine, W.E., Elliker, P.R., Lipids of antibiotic-sensitive and resistant strains of Pseudomonas aeruginosa (1971) Can. J. Microbiol, 17, pp. 1357-1365 Cirillo, V.F., Wilkins, F.O., Use of uranil ion in membrane transport studies (1964) J. Bacteriol, 87, pp. 232-233 Fiechter, A., Fuhrmann, G.F., Kappeli, O., Regulation of glucose metabolism in growing yeast cells (1981) Adv Microb Physiol., 22, pp. 123-183 Gilby, A.R., Few, A.V., Lysis of protoplasts of Micrococcus lysodeikticus by ionic detergents (1960) J. Gen. Microbiol., 23, pp. 19-25 Hugo, W.B., Observations on the manometric estimation of bacterial activity (1952) Proc. Soc. Appl. Bact., 15, pp. 29-33 Hugo, W.B., The mode of action of antibacterial agents (1967) J. Appl Bacteriol., 30, pp. 17-50 Maxwell, W.A., Metzler, R., Spoerl, E., Uranyl nitrate inhibition of transport systems in Saccharomyces cerevisiae (1981) J Bacteriol., 105, p. 1205 Miller, B.J., Baker, Z., Hamson, R.W., Action of a quaternary ammonium type of wetting agent on metabolism of microorganisms associated with dental caries (1939) Proc. Soc. Exp. Biol Med., 42, pp. 705-706 Nikaido, H., Nakae, T., The outer membrane of Gram-negative bacteria (1979) Adv. Microb. Physiol, 20, pp. 163-250 Nikaido, H., Hancock, R.E.W., Outer membrane permeability of Pseudomonas aeruginosa (1986) The Biology of Pseudomonas, 10, pp. 145-194. , Sokatch, J.R (Ed.). Academic Press., Inc Ordal, E.J., Harvorson, H.O., A comparison of hydrogen production from sugars and formic acid by normal and variant strains of Escherichia coli (1939) J Bacteriol, 38, pp. 199-220 Ordal, E.J., Borg, A.F., Effect of surface active agents on oxydations of lactate by bacteria (1942) Proc. Soc. Exp Biol. Mea, 50, pp. 332-336 Salton, M.R.J., Lytic agents, cell permeability, and monolayer permeability (1968) J. Gen. Physiol., 52, pp. 227s-252s Sevag, M.G., Ross, O.A., Studies on the mechanisms of the inhibitory action of zephiran on yeast cells (1944) J. Bacteriol, 48, pp. 677-682 Tomlinson, E., Brown, M.R.W., Davis, S.S., Effect of colloidal association on the activity of alkylbenzy ldimethylammonium chloride against Pseudomonas aeruginosa (1977) J Med. Chem, 20, p. 1277 Ueno, M., Yokoya, F., Formulation of culture medium for growth of microbes from the sugar cane industry and use of cetyltrimethylammonium bromide as antimicrobial agent (1994) Rev Microbiol, 25, pp. 261-269