Artículos de revistas
Isolation And Characterization Of Isolectins From Talisia Esculenta Seeds
Registro en:
Protein Journal. , v. 20, n. 6, p. 495 - 500, 2001.
15723887
2-s2.0-52549089663
Autor
Freire M.D.G.M.
Machado O.L.T.
Smolka M.B.
Marangoni S.
Novello J.C.
Macedo M.L.R.
Institución
Resumen
Four isolectins (TEL-I, TEL-II, TEL-III and TEL-IV) were isolated from seeds of Talisia esculenta by reverse-phase high-performance liquid chromatography. RP-HPLC was performed on a u-Bondapack C18 column (0.78 cm X 30 cm) (Waters 991-PDA system) at room temperature. Rechromatography of the four fractions on a C18 column under the same conditions yielded lectins with two dissimilar subunits (M r 20 kDa and 40 kDa) bound noncovalently. The isolectins showed very similar characteristics, such as molecular masses, N-terminal sequences, and hemagglutinating activity, but differed in their isoelectric points and in inhibition by carbohydrates. © 2001 Plenum Publishing Corporation. 20 6 495 500 Abrecht, H., Wattiez, R., Ruysschaert, J.-M., Homblé, F., (2000) Plant Physiol., 124, pp. 1181-1190 Animashaun, T., Togun, R.A., Hughes, R.C., (1994) Glycoconjugate J., 11, pp. 299-303 Anuradha, P., Bhide, S.V., (1999) Phytochemistry, 52, pp. 751-758 Barbieri, L., Falasca, A., Franceschi, C., Licastro, F., Rossi, C.A., Stirpe, F., (1983) Biochem. J., 215, pp. 433-439 Blum, H., Beier, H., Gross, H.J., (1987) Electrophoresis, 8, pp. 93-99 Cavada, B.S., Santos, C.F., Granjeiros, T.B., Nunes, E.P., Sales, P.V.P., Ramos, R.L., Sousa, F.A.M., Calvete, J.J., (1998) Phytochemistry, 49, pp. 675-680 Datta, P.K., Figueroa, M.O.R., Lajolo, F.M., (1993) J. Agric. Food Chem., 41, pp. 1851-1855 Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., (1956) Anal. Chem., 28, pp. 350-356 Eloumani, H., Bladier, D., Caruelle, D., Courty, J., Joubert, R., Caron, M., (1990) Int. J. Biochem., 22, pp. 539-544 Freire, M.G.M., Gomes, V.M., Corsini, R.E., Machado, O.L.T., De Simone, S.G., Novello, J.C., Marangoni, S., Macedo, M.L.R., Plant Physiol. Biochem., , in press Harata, K., Muraki, M., (2000) J. Mol. Biol., 297, pp. 673-681 Kilpatrick, D.C., Weston, J., Urbaniak, S.J., (1983) Anal. Biochem., 134, pp. 205-209 Laemmli, U.K., (1970) Nature, 227, pp. 680-689 Machuka, J.S., Okeola, O.G., Van Damme, J.M., Chrispeels, M.J., Leuven, F.V., Peumans, W.J., (1999) Phytochemistry, 51, pp. 721-728 Matsumoto, I., Koyama, T., Kitagaki-Ogawa, H., Seno, N., (1987) J. Chromatog., 400, pp. 77-81 Ooi, L.S.M., Ng, T.B., Sun, S.S.M., Ooi, V.E.C., (2000) J. Protein Chem., 19, pp. 163-168 Pérez, G., (1998) Int. J. Biochem. Cell Biol., 30, pp. 843-853 Pratt, R.C., Singh, N.K., Shade, R.E., Murdock, L.L., Bressan, R.A., (1990) Plant Physiol., 93, pp. 1453-1459 Rüdiger, H., (1997) Glycosciences, Status and Perpectives, 2, pp. 415-438. , H-J. Gabius and S. Gabius (Eds): London, Chapman & Hall, Weinheim Rüdiger, H., (1998) Acta Anat., 161, pp. 130-152 Santos-de-Oliveira, R., Dias-Baruffi, M., Thomaz, S.M.O., Beltramini, L.M., Roque-Barreira, M.C., (1994) J. Immunol., 22, pp. 1798-1807 Suzuki, T., Takagi, T., Furukohri, T., Kawamura, K., Nakauchi, M., (1990) J. Biol. Chem., 265, pp. 1274-1281 Wongkham, S., Wongkham, C., Boonsiri, P., Simasathiansophon, S., Trisonthi, C., Atisook, K., (1995) Phytochemistry, 40, pp. 1331-1334