Artículos de revistas
Comparative Biochemical Studies Of Myotoxic Phospholipase A2 From Bothrops Venom
Registro en:
Protein And Peptide Letters. , v. 8, n. 3, p. 179 - 186, 2001.
9298665
10.2174/0929866013409445
2-s2.0-0034971240
Autor
Toyama M.H.
Soares A.M.
Andriao-Escarso S.H.
Novello J.C.
Oliveira B.
Giglio J.R.
Fontes M.R.M.
Marangoni S.
Institución
Resumen
Venoms from Bothrops jararacussu, Bothrops asper, Bothrops atrox, Bothrops pirajai, Bothrops moojeni, Bothrops alternatus and Bothrops (Bothriopsis) bilineata were fractionated using a simplified procedure based on ion-exchange chromatography on CM-Sepharose at pH 8.0 or reverse phase HPLC. The resulting elution profiles showed important differences in the myotoxin content of these venoms. The venoms from B. alternatus, B. atrox and Bothriopsis bilineata did not contain the major myotoxin found in the other venoms. The amino acid sequence of the first 50 residues of the N-terminal region of the PLA2-like myotoxins showed a homology of 90-96% with other bothropic myotoxins. All of the myotoxins isolated induced rat paw edema, increased the level of plasma creatine kinase and produced myonecrosis together with polymorphonuclear cell infiltration. 8 3 179 186 Hoge, A.R., Romano Hoge, S.A.R.W.L., (1978) Mem. Inst. Butantan, 42-43, pp. 373-496 Chang, L.-S., Kuo, K.-W., Lin, S.-R., Chang, C.-C., (1994) J. Protein Chem., 13, pp. 641-648 Shimohigashi, Y., Tani, A., Matsumoto, H., Nakashima, K.-I., Yamaguchi, Y., Oda, N., Takano, Y., Ohno, M., (1995) J. Biochem. (Tokyo) Biochem., 118, pp. 1037-1044 Ogawa, T., Nakashima, K.-I., Nobushima, I., Deshimaru, M., Shimohigashi, Y., Fukumaki, Y., Sakaki, Y., Ohno, M., (1996) Toxicon, 34, pp. 1229-1236 Dennis, E.A., (1994) Biol. Chem., 269, pp. 13057-13060 Araújo, H.S., White, S.P., Ownby, C.L., (1996) Toxicon, 34, pp. 1237-1242 Kini, R.M., Iwanaga, S., (1986) Toxicon, 24, pp. 895-905 Arni, R.K., Ward, R.J., (1996) Toxicon, 34, pp. 827-841 Mancuso, L.C., Correa, M.M., Vieira, C.A., Cunha, O.A.B., Lachat, J.J., Selistre, H.S.A., Ownby, C.L., Giglio, J.R., (1995) Toxicon, 33, pp. 615-626 Soares, A.M., Rodrigues, V.M., Homsi-Brandeburgo, M.I., Toyama, M.H., Lombard, F.R., Arni, A.K., Giglio, J.R., (1998) Toxicon, 36, pp. 503-514 Gutiérrez, J.M., Lomonte, B., (1995) Toxicon, 33, pp. 1405-1424 Fletcher, J.E., Humbert, M., Wieland, S.J., Gong, Q.H., Jiang, M.S., (1996) Toxicon, 34, pp. 1301-1311 Cho, W., Kezdy, F.J., (1991) Methods Enzymol., 197, pp. 75-79 Holzer, M., Mackessy, S.P., (1996) Toxicon, 35, pp. 1149-1155 Marangoni, S., Toyama, M.H., Arantes, E.C., Giglio, J.R., Da Silva, C.A., Carneiro, E.M., Gonçalves, A.A., Oliveira, B., (1995) Biophys. Acta, 1243, pp. 309-314 Higgins, D.G., Sharp, P.M., (1989) Comput. Appl. Biosci., 5, pp. 151-153 Araújo, A.L., Radvanyi, F., Bon, C., (1994) Toxicon, 32, pp. 1069-1081 Fukagawa, T., Nose, T., Shimohigashi, Y., Ogawa, T., Oda, N., Nakashima, K.I., Chang, C.C., Ohno, M., (1993) Toxicon, 31, pp. 957-967 Homsi-Brandeburgo, M.I., Queiroz, L.S., Santo-Neto, H., Rodrigues-Simioni, L., Giglio, J.R., (1988) Toxicon, 26, pp. 615-627 Nakai, M., Nakashima, K.I., Ogawa, T., Shimohigashi, Y., Hattori, S., Chang, C.C., Ohno, M., (1995) Toxicon, 33, pp. 1469-1478 Toyama, M.H., Soares, A.M., Vieira, C.A., Novello, J.C., Oliveira, B., Giglio, J.R., Marangoni, S., (1998) J. Protein Chem., 17, pp. 713-718 Soares, A.M., Anzaloni Pedrosa, L.H., Fontes, M.R.M., Da Silva, R.J., Giglio, J.R., (1998) J. Venom. Anim. Toxins, 4. , in press De Azevedo W.F., Jr., Ward, R.J., Gutierrez, J.M., Arni, R.K., (1998) Toxicon, pp. 1395-1406 Francis, B., Gutiérrez, J.M., Lomonte, B., Kaiser, I.I., (1991) Arch. Biochem. Biophys., 284, pp. 352-359 Toyama, M.H., Mancuso, L.C., Giglio, J.R., Novello, J.C., Oliveira, B., Marangoni, S., (1995) Biochem. Mol. Biol. Inter., 37, pp. 1047-1055 Heirikson, R.L., Trueger, E.T., Kein, P.S., (1977) J. Biol. Chem., 252, pp. 4913-4921 Lomonte, B., Gutiérrez, J.M., Mata, E., (1985) Toxicon, 23, pp. 807-813 Cintra, A.C.O., Marangoni, S., Oliveira, B., Giglio, J.R., (1993) J. Protein Chem., 12, pp. 57-64 Kaiser, I.I., Gutiérrez, J.M., Plummer, D., Aird, S.D., Odell, G.V., (1990) Arch. Biochem. Biophys., 278, pp. 319-325 Gowda, V.T., Schmidt, J., Middlebrook, J.L., (1994) Toxicon, 32, pp. 665-673