Artículos de revistas
E. Coli Virulence Factors In Children With Neurogenic Bladder Associated With Bacteriuria
Registro en:
Pediatric Nephrology. , v. 21, n. 3, p. 376 - 381, 2006.
0931041X
10.1007/s00467-005-2133-0
2-s2.0-33244472620
Autor
Guidoni E.B.M.
Dalpra V.A.
Figueiredo P.M.
da Silva Leite D.
Mimica L.M.J.
Yano T.
Blanco J.E.
Toporovski J.
Institución
Resumen
The value of E. coli virulence factors in patients with neurogenic bladder has not been established. The aim of this study is to correlate E. coli virulence factors with asymptomatic and symptomatic UTI in children with neurogenic bladder. Fifty E. coli strains, which were collected in sequence, underwent analysis in relation to: The association to pyuria, serotype (O:H), the presence of genes and expression of fimbriae P, type 1, S and hemagglutinin Dr, the presence of the gene and production of hemolysins and cytotoxins (CNF1). We also analyzed the cell adherence capability and pattern and presence of usp (uropathogenic-specific protein). Pyuria was present in most of the positive urine cultures, with 86% AB and 97% UTI. Low rates of uropathogenic strains were observed in the two groups, with 18% AB and 21% UTI. Type 1 fimbria predominated in 44% of the E. coli strains. Of the bacteria studied, 30% (15 strains) exhibited papG genotypes (11 class II and 4 class III). Of these, 12/15 patients presented AB. Production of hemolysins was detected in 38% of the strains (16 AB and 3 UTI) and usp in only 18% of the strains, with 8 AB and 1 UTI. Adherence tests demonstrated the adhesive capacity in all samples analyzed. Neither group (AB or symptomatic UTI) presented a statistically significant difference in relation to the virulence factors studied. E. coli clones that caused symptomatic UTI in children with neurogenic bladder expressed few virulence factors, with no statistically significant difference in comparison to the AB group. © IPNA 2005. 21 3 376 381 Lapides, J., Diokno, A.C., Silber, S.J., Lowe, B.S., Clean, intermittent self-catheterization in the treatment of urinary tract disease (1972) J Urol, 107, pp. 458-461 Joseph, D.B., Bauer, S.B., Colodny, A.H., Mandell, J., Retik, A.B., Clean, intermittent catheterization of infants with neurogenic bladder (1989) Pediatrics, 84, pp. 78-82 Kass, E.J., Koff, S.A., Diokno, A.C., Lapides, J., The significance of bacilluria in children on long-term intermittent catheterization (1981) J Urol, 126, pp. 223-225 Schlager, T.A., Dilks, S., Trudell, J., Whittam, T.S., Hendley, J.O., Bacteriuria in children with neurogenic bladder treated with intermittent catheterization: Natural history (1995) J Pediatr, 126, pp. 490-496 Schlager, T.A., Whittam, T.S., Hendley, J.O., Wilson, R.A., Bhang, J., Grady, R., Stapleton, A., Expression of virulence factors among Escherichia coli isolated from the periurethra and urine of children with neurogenic bladder on intermittent catheterization (2000) Pediatr Infect Dis J, 19, pp. 37-41 Brem, A.S., Martin, D., Callaghan, J., Maynard, J., Long-term renal risk factors in children with meningomyelocele (1987) J Pediatr, 110, pp. 51-55 Schlager, T.A., Dilks, S.A., Lohr, J.A., Hayden, G.F., Kopco, J.A., Hendley, J.W., Periurethral colonization and urinary leukocytes as markers for bacteriuria in children with neurogenic bladder (1992) Urol Res, 20, pp. 361-363 Stöher, M., Castro- Diaz, D., Chartier-Kartler, E., Guidelines on neurogenic lower urinary tract dysfunction (2003), http//www.uroweb.org/files/uploaded_files/guidelines/neurogenic.pdf, European Association of Urology (cited 2003 Feb 2). Available fromHellerstein, S., Recurrent urinary tract infections in children (1982) Pediatr Infect Dis, 1, pp. 271-281 Kass, E.H., Asymptomatic infections of the urinary tract (1956) Trans Assoc Am Physicians, 69, pp. 56-64 Blanco, J., Blanco, M., Alonso, M.P., A Factores de virulência de los Escherichia coli causantes de infecciones extraintestinales (1992) Monografias Da Universidade De Compostela No, 170, pp. 87-90 Evans Jr., D.J., Evans, D.G., Young, L.S., Pitt, J., Hemagglutination typing of Escherichia coli: Definition of seven hemagglutination types (1981) J Clin Microbiol, 12, pp. 235-242 Gander, R.M., Thomas, V.L., Forland, M., Mannose-resistant hemagglutination and P receptor recognition of uropathogenic Escherichia coli isolated from adult patients (1985) J Infect Dis, 151, pp. 508-513 Blanco, J., Blanco, M., Gonzalez, E.A., Alonso, M.P., Garabal, J.I., Comparative evaluation of three tests for the detection of Escherichia coli cytotoxic necrotizing factors (CNF1 and CNF2) using filtrates of cultures treated with mitomycin C (1990) FEMS Microbiol Lett, 57, pp. 311-316 Scaletsky, I.C., Silva, M.L., Trabulsi, L.R., Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells (1984) Infect Immun, 45, pp. 534-536 Blanco, M., Blanco, J.E., Rodrigues, E., Detection of virulence genes in uropathogenic Escherichia coli by polymerase chain reaction (PCR): Comparison with results obtained using phenotypic methods (1997) J Microbiol Methods, 31, pp. 37-43 Yamamoto, S., Terai, A., Yuri, K., Kurazono, H., Takeda, Y., Yoshida, O., Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction (1995) FEMS Immunol Med Microbiol, 12, pp. 85-90 Daigle, F., Harel, J., Fairbrother, J.M., Lebel, P., Expression and detection of pap-, sfa-, and afa-encoded fimbrial adhesin systems among uropathogenic Escherichia coli (1994) Can J Microbiol, 40, pp. 286-291 Pickett, C.L., Twiddy, E.M., Coker, C., Holmes, R.K., Cloning, nucleotide sequence, and hybridization studies of the type IIb heat-labile enterotoxin gene of Escherichia coli (1989) J Bacteriol, 171, pp. 4945-4952 Karkkianen, U.M., Kauppinen, J., Ikaheimo, R., A Rapid and specific detection of three different G adhesin classes of P-fimbriae in uropathogenic Escherichia coli by polimerase chain reaction (1998) J Microl Method, 34, pp. 23-29 Kurazono, H., Yamamoto, S., Nakano, M., Nair, G.B., Terai, A., Chaicumpa, W., Hayashi, H., Characterization of a putative virulence island in the chromosome of uropathogenic Escherichia coli possessing a gene encoding a uropathogenic-specific protein (2000) Microb Pathog, 28, pp. 183-189 Rosner, B., (1986) Fundamentals of Biostatistics, , 2nd edn. PWS Publishers, Boston Sotolongo Jr., J.R., Koleilat, N., Significance of asymptomatic bacteriuria in spinal cord injury patients on condom catheter (1990) J Urol, 143, pp. 979-980 Andersson, P., Engberg, I., Lidin-Janson, G., Lincoln, K., Hull, R., Hull, S., Svanborg, C., Persistence of Escherichia coli bacteriuria is not determined by bacterial adherence (1991) Infect Immun, 59, pp. 2915-2921 Gribble, M.J., McCallum, N.M., Schechter, M.T., Evaluation of diagnostic criteria for bacteriuria in acutely spinal cord injured patients undergoing intermittent catheterization (1988) Diagn Microbiol Infect Dis, 9, pp. 197-206 Schlager, T.A., Grady, R., Mills, S.E., Hendley, J.O., Bladder epithelium is abnormal in patients with neurogenic bladder due to myelomeningocele (2004) Spinal Cord, 42, pp. 163-168 Orskov, F., Sharma, V., Orskov, I., Influence of growth temperature on the development of Escherichia coli polysaccharide K antigens (1984) J Gen Microbiol, 130, pp. 2681-2684 Hansson, S., Jodal, U., Lincoln, K., Svanborg-Eden, C., Untreated asymptomatic bacteriuria in girls: II-Effect of phenoxymethylpenicillin and erythromycin given for intercurrent infections (1989) BMJ, 298, pp. 856-859 Eisenstein, B.I., Phase variation of Type 1 fimbriae in Escherichia coli is under transcriptional control (1981) Science, 214, pp. 337-339 Miyazaki, J., Ba-Thein, W., Kumao, T., Obata Yasuoka, M., Akaza, H., Hayshi, H., Type 1, P and S fimbriae, and afimbrial adhesin I are not essential for uropathogenic Escherichia coli to adhere to and invade bladder epithelial cells (2002) FEMS Immunol Med Microbiol, 33, pp. 23-26 Mobley, H.L., Chippendale, G.R., Tenney, J.H., Hull, R.A., Warren, J.W., Expression of type 1 fimbriae may be required for persistence of Escherichia coli in the catheterized urinary tract (1987) J Clin Microbiol, 25, pp. 2253-2257 Plos, K., Connell, H., Jodal, U., Marklund, B.I., Marild, S., Wettergren, B., Svanborg, C., Intestinal carriage of P fimbriated Escherichia coli and the susceptibility to urinary tract infection in young children (1995) J Infect Dis, 171, pp. 625-631 Puente, J.L., Finlay, B.B., Pathogenic Escherichia coli (2001) Principles of Bacterial Pathogenesis, pp. 387-457. , Groisman EA (ed) Academic Press, San Diego Roberts, J.A., Marklund, B.I., Ilver, D., Haslam, D., Kaack, M.B., Baskin, G., Louis, M., Normark, S., The Gal(alpha 1-4)Gal-specific tip adhesin of Escherichia coli P-fimbriae is needed for pyelonephritis to occur in the normal urinary tract (1994) Proc Natl Acad Sci USA, 91, pp. 11889-11893 Tseng, C.C., Huang, J.J., Ko, W.C., Yan, J.J., Wu, J.J., Decreased predominance of papG class II allele in Escherichia coli strains isolated from adults with acute pyelonephritis and urinary tract abnormalities (2001) J Urol, 166, pp. 1643-1646 Andreu, A., Stapleton, A.E., Fennell, C., Lockman, H.A., Xercavins, M., Fernandez, F., Stamm, W.E., Urovirulence determinants in Escherichia coli strains causing prostatitis (1997) J Infect. Dis, 176, pp. 464-469 Foxman, B., Zhang, L., Palin, K., Tallman, P., Marrs, C.F., Bacterial virulence characteristics of Escherichia coli isolates from first-time urinary tract infection (1995) J Infect Dis, 171, pp. 1514-1521 Nakano, M., Yamamoto, S., Terai, A., Ogawa, O., Makino, S.I., Hayashi, H., Nair, G.B., Kurazono, H., Structural and sequence diversity of the pathogenicity island of uropathogenic Escherichia coli which encodes the USP protein (2001) FEMS Microbiol Lett, 205, pp. 71-76