Artículos de revistas
Decreased Insulin Secretion In Islets From Rats Fed A Low Protein Diet Is Associated With A Reduced Pkaα Expression
Registro en:
Journal Of Nutrition. , v. 134, n. 1, p. 63 - 67, 2004.
223166
2-s2.0-0347986915
Autor
Ferreira F.
Barbosa H.C.L.
Stoppiglia L.F.
Delghingaro-Augusto V.
Pereira E.A.
Boschero A.C.
Carneiro E.M.
Institución
Resumen
A low protein diet has been shown to affect the amount and activity of several enzymes and to decrease insulin secretion by islets isolated from rats fed such a diet. To understand the mechanisms involved in this phenomenon, we investigated the effects of forskolin, a stimulator of adenylyl cyclase, on insulin secretion by pancreatic islets from rats fed a normal (17%; NP) or low (6%; LP) protein diet for 8 wk. Isolated islets were incubated for 1 h in Krebs-bicarbonate solution containing 8.3 mmol glucose/L, with or without 10 μmol forskolin/L. The forskolin-induced insulin secretion was higher in islets from NP rats than in those from LP rats (P < 0.05). Western blotting revealed that the amount of the α catalytic subunit of protein kinase A (PKAα) was 35% lower in islets from LP rats than in islets from NP rats (P < 0.05). Moreover, PKAα mRNA expression was reduced by 30% in islets from LP rats (P < 0.05). Our results indicated a possible relationship between a low protein diet and a reduction in PKAα expression. These alterations in PKAα may be responsible in part for the decreased insulin secretion by islets from rats fed a low protein diet. 134 1 63 67 Cleator, J., Wilding, J., Obesity and diabetes (2003) Nurs. Times, 99, pp. 54-55 Ferreira, F., Filiputti, E., Arantes, V.C., Stoppiglia, L.F., Araujo, E.P., Delghingaro-Augusto, V., Latorraca, M.Q., Carneiro, E.M., Decreased cholinergic stimulation of insulin secretion by islets from rats fed a low protein diet is associated with reduced protein kinaseα expression (2003) J. Nutr., 133, pp. 695-699 Reis, M.A., Carneiro, E.M., Mello, M.A., Boschero, A.C., Saad, M.J., Velloso, L.A., Glucose-induced insulin secretion is impaired and insulin-induced phosphorylation of the insulin receptor and insulin receptor substrate-1 are increased in protein-deficient rats (1997) J. Nutr., 127, pp. 403-410 Latorraca, M.Q., Reis, M.A., Carneiro, E.M., Mello, M.A., Velloso, L.A., Saad, M.J.A., Boschero, A.C., Protein deficiency and nutritional recovery modulate insulin secretion and the early steps of insulin action in rats (1998) J. Nutr., 128, pp. 1643-1649 Swenne, I., Crace, C.J., Milner, R.D., Persistent impairment of insulin secretory response to glucose in adult rats after limited period of protein-calorie malnutrition early in life (1987) Diabetes, 36, pp. 454-458 Escriva, F., Rodriguez, C., Cacho, J., Alvarez, C., Portha, B., Pascual-Leone, A.M., Glucose utilization and insulin action in adult rats submitted to prolonged food restriction (1992) Am. J. Physiol., 263, pp. E1-E7 Okitolonda, W., Brichard, S.M., Henquin, J.C., Repercussions of chronic protein-calorie malnutrition on glucose homeostasis in the rat (1987) Diabetologia, 30, pp. 946-951 Picarel-Blanchot, F., Alvarez, C., Bailbe, D., Pascual-Leone, A.M., Portha, B., Changes in insulin action and insulin secretion in the rat after dietary restriction early in life: Influence of food restriction versus low-protein food restriction (1995) Metabolism, 44, pp. 1519-1526 Escriva, F., Kergoat, M., Bailbe, D., Pascual-Leone, A.M., Portha, B., Increased insulin action in the rat after protein malnutrition early in life (1991) Diabetologia, 34, pp. 559-564 Levine, L.S., Wright, P.G., Marcus, F., Failure to secrete immunoreactive insulin by rats fed a low protein diet (1983) Acta Endocrinol., 102, pp. 240-245 Swenne, I., Borg, L.A., Crace, C.J., Schnell Landstrom, A., Persistent reduction of pancreatic beta-cell mass after a limited period of protein-energy malnutrition in the young rat (1992) Diabetologia, 35, pp. 939-945 Cherif, H., Reusens, B., Dahri, S., Remacle, C., A protein-restricted diet during pregnancy alters in vitro insulin secretion from islets of fetal Wistar rats (2001) J. Nutr., 131, pp. 1555-1559 Araujo, E.P., Amaral, M.E., Souza, C.T., Bordin, S., Ferreira, F., Saad, M.J., Boschero, A.C., Velloso, L.A., Blockade of IRS1 in isolated rat pancreatic islets improves glucose-induced insulin secretion (2002) FEBS Lett., 531, pp. 437-442 Satin, L.S., Kinard, T.A., Neurotransmitters and their receptors in the islets of Langerhans of the pancreas: What messages do acetylcholine, glutamate, and GABA transmit? (1998) Endocrine, 8, pp. 213-223 Nesher, R., Anteby, E., Yedovizky, M., Warwar, N., Kaiser, N., Cerasi, E., Beta-cell protein kinases and the dynamics of the insulin response to glucose (2002) Diabetes, 51 (SUPPL. 1), pp. S68-S73 Krebs, E.G., Beavo, J.A., Phosphorylation-dephosphorylation of enzymes (1979) Annu. Rev. Biochem., 48, pp. 923-959 Meinkoth, J.L., Ji, Y., Taylor, S.S., Feramisco, J.R., Dynamics of the distribution of cyclic AMP-dependent protein kinase in living cells (1990) Proc. Natl. Acad. Sci. U.S.A., 87, pp. 9595-9599 Takahashi, N., Kadowaki, T., Yazaki, Y., Miyashita, Y., Kasai, H., Multiple exocytotic pathways in pancreatic beta cells (1997) J. Cell. Biol., 138, pp. 55-64 Griffioen, G., Thevelein, J.M., Molecular mechanisms controlling the localisation of protein kinase A (2002) Curr. Genet., 41, pp. 199-207 Szecowka, J., Grill, V., Sandberg, E., Efendic, S., Effect of GIP on the secretion of insulin and somatostatin and the accumulation of cyclic AMP in vitro in the rat (1982) Acta Endocrinol., 99, pp. 416-421 Thorens, B., GLP-1 and the control of insulin secretion (1994) J. Annu. Diabetol. Hotel Dieu, pp. 33-46 Doyle, M.E., Egan, J.M., Pharmacological agents that directly modulate insulin secretion (2003) Pharmacol. Rev., 55, pp. 105-131 Morgan, L.M., Hampton, S.M., Tredger, J.A., Cramb, R., Marks, V., Modifications of gastric inhibitory polypeptide (GIP) secretion in man by a high-fat diet (1988) Br. J. Nutr., 59, pp. 373-380 O'Brien, L.J., Levac, K.D., Nagy, L.E., Moderate dietary protein and energy restriction modulate cAMP-dependent protein kinase activity in rat liver (1998) J. Nutr., 128, pp. 927-933 Dumas, B.T., Watson, W.A., Biggs, H.G., Albumin standards and the measurement of serum albumin with bromocresol green (1997) Clin. Chim. Acta, 258, pp. 21-30 Heard, C.R., Frangi, S.M., Wright, P.M., Biochemical characteristics of different forms of protein-energy malnutrition in rats (1973) Proc. Nutr. Soc., 32, pp. 47A. , abs Weinkove, C., Weinkove, E.A., Pimstone, B.L., Glucose tolerance and insulin release in malnourished rats (1976) Clin. Sci. Mol. Med., 50, pp. 153-163 Bordin, S., Boschero, A.C., Carneiro, E.M., Atwater, I., Ionic mechanisms involved in the regulation of insulin secretion by muscarinic agonists (1995) J. Membr. Biol., 148, pp. 177-184 Scott, A.M., Atwater, I., Rojas, E., A method for the simultaneous measurement of insulin release and B cell membrane potential in single mouse islets of Langerhans (1981) Diabetologia, 21, pp. 470-475 Amaral, M.E., Ueno, M., Carvalheira, J.B., Carneiro, E.M., Velloso, L.A., Saad, M.J., Boschero, A.C., Prolactin-signal transduction in neonatal rat pancreatic islets and interaction with the insulin-signaling pathway (2003) Horm. Metab. Res., 35, pp. 282-289 Kelley, G.G., Zawalich, K.C., Zawalich, W.S., Synergistic interaction of glucose and neurohumoral agonists to stimulate islet phosphoinositide hydrolysis (1995) Am. J. Physiol., 269, pp. E575-E582 Verspohl, E.J., Tacke, R., Mutschler, E., Lambrecht, G., Muscarinic receptor subtypes in rat pancreatic islets: Binding and functional studies (1990) Eur. J. Pharmacol., 178, pp. 303-311 Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254 Eickhoff, B., Korn, B., Schick, M., Poustka, A., Van Der Bosch, J., Normalization of array hybridization experiments in differential gene expression analysis (1999) Nucleic Acids Res., 27, pp. e33. , abs Heard, C.R., The effects of protein-energy malnutrition on blood glucose homeostasis (1978) World Rev. Nutr. Diet., 30, pp. 107-147 Claeyssens, S., Lavoinne, A., Vaillant, C., Rakotomanga, J.A., Bois-Joyeux, B., Peret, J., Metabolic changes during early starvation in rats fed a low-protein diet in the postweaning growth period (1992) Metabolism, 41, pp. 722-727 Srinivasan, M., Aalinkeel, R., Song, F., Lee, B., Laychock, S.G., Patel, M.S., Adaptive changes in insulin secretion by islets from neonatal rats raised on a high-carbohydrate formula (2000) Am. J. Physiol., 279, pp. E1347-E1357 Jones, P.M., Persaud, S.J., Protein kinases, protein phosphorylation, and the regulation of insulin secretion from pancreatic beta-cells (1998) Endocr. Rev., 19, pp. 429-461 Wang, X., Zhou, J., Doyle, M.E., Egan, J.M., Glucagon-like peptide-1 causes pancreatic duodenal homeobox-1 protein translocation from the cytoplasm to the nucleus of pancreatic beta-cells by a cyclic adenosine monophosphate/protein kinase A-dependent mechanism (2001) Endocrinology, 142, pp. 1820-1827 Yamazaki, T., Komuro, I., Zou, Y., Kudoh, S., Mizuno, T., Hiroi, Y., Shiojima, I., Yazaki, Y., Protein kinase A and protein kinase C synergistically activate the Raf-1 kinase/mitogen-activated protein kinase cascade in neonatal rat cardiomyocytes (1997) J. Mol. Cell. Cardiol., 29, pp. 2491-2501 Huang, C.J., Feltkamp, D., Nilsson, S., Gustafsson, J.A., Synergistic activation of RLD-1 by agents triggering PKA and PKC dependent signalling (1998) Biochem. Biophys. Res. Commun., 243, pp. 657-663 Wei, Y., Tae, N., Tatsuya, F., Hiroyoshi, H., Takao, S., Yasuharu, S., Ighiro, N., Synergism of protein kinase A, protein kinase C, and myosin light-chain kinase in the secretory cascade of the pancreatic B-cell (2000) Diabetes, 49, pp. 945-952 Simonsson, E., Karlsson, S., Ahren, B., The cyclic AMP-protein kinase A pathway restrains islet phospholipase A2 activation (2000) Biochem. Biophys. Res. Commun., 269, pp. 242-246 Ammon, H.P., Muller, A.B., Effect of forskolin on islet cyclic AMP, insulin secretion, blood glucose and intravenous glucose tolerance in rats (1984) Naunyn-Schmiedebergs Arch. Pharmakol., 326, pp. 364-367 Stephen, L.L., Nagy, L.E., Very low protein diets induce a rapid decrease in hepatic cAMP-dependent protein kinase followed by a lower increase in adenylyl cyclase activity in rats (1996) J. Nutr., 126, pp. 1799-1807 Bruhat, A., Jousse, C., Fafournoux, P., Amino acid limitation regulates gene expression (1999) Proc. Nutr. Soc., 58, pp. 625-632 Jousse, C., Bruhat, A., Fafournoux, P., Amino acid regulation of gene expression (1999) Curr. Opin. Clin. Nutr. Metab. Care, 2, pp. 297-301 Jousse, C., Bruhat, A., Ferrara, M., Fafournoux, P., Evidence for multiple signaling pathways in the regulation of gene expression by amino acids in human cell lines (2000) J. Nutr., 130, pp. 1555-1560 Harrison, A.P., Tivey, D.R., Clausen, T., Duchamp, C., Dauncey, M.J., Role of thyroid hormones in early postnatal development of skeletal muscle and its implications for undernutrition (1996) Br. J. Nutr., 76, pp. 841-855 Jahnsen, T., Lohmann, S.M., Walter, U., Hedin, L., Richards, J.S., Purification and characterization of hormone-regulated isoforms of the regulatory subunit of type II cAMP-dependent protein kinase from rat ovaries (1985) J. Biol. Chem., 260, pp. 15980-15987 Oyen, O., Sandberg, M., Eskild, W., Levy, F.O., Knutsen, G., Beebe, S., Hansson, V., Jahnsen, T., Differential regulation of messenger ribonucleic acids for specific subunits of cyclic adenosine 3′, 5′-monophosphate (cAMP)-dependent protein kinase by cAMP in rat Sertoli cells (1988) Endocrinology, 122, pp. 2658-2666 Levy, F.O., Ree, A.H., Eikvar, L., Govindan, M.V., Jahnsen, T., Hansson, V., Glucocorticoid receptors and glucocorticoid effects in rat Sertoli cells (1989) Endocrinology, 124, pp. 430-436 Tasken, K.A., Knutsen, H.K., Eikvar, L., Tasken, K., Eskild, W., Jahnsen, T., Hansson, V., Protein kinase C activation by 12-O-tetradecanoyl-phorbol 13-acetate modulates messenger ribonucleic acid levels for two of the regulatory subunits of 3′, 5′-cyclic adenosine monophosphate-dependent protein kinases (RII beta and RI alpha) via multiple and distinct mechanisms (1992) Endocrinology, 130, pp. 1271-1280 Tasken, K., Kvale, D., Hansson, V., Jahnsen, T., Protein kinase C activation selectively increases mRNA levels for one of the regulatory subunits (RI alpha) of cAMP-dependent protein kinases in HT-29 cells (1990) Biochem. Biophys. Res. Commun., 172, pp. 409-414 Reeves, P.G., Nielsen, F.H., Fahey Jr., C.G., AIN-93 purified diets for laboratory rodents: Report of the American Institute of Nutrition ad hoc working committee on the reformulation of the AIN-76 rodent diet (1993) J. Nutr., 123, pp. 1939-1951