Actas de congresos
Bromelain Partitioning In Two-phase Aqueous Systems Containing Peo-ppo-peo Block Copolymers
Registro en:
Journal Of Chromatography B: Analytical Technologies In The Biomedical And Life Sciences. , v. 807, n. 1, p. 61 - 68, 2004.
15700232
10.1016/j.jchromb.2004.03.029
2-s2.0-2642511370
Autor
Rabelo A.P.B.
Tambourgi E.B.
Pessoa Jr. A.
Institución
Resumen
Bromelain is an enzymatic complex obtained from pineapple (Ananas comosus) fruits and stem. Thermoseparation of bromelain by poly(ethylene oxide) (PEO)- poly(propylene oxide) (PPO)- poly(ethylene oxide) (PEO) block copolymers aqueous solutions was studied. Triblock copolymers with different EO percentages and different molecular mass were evaluated. Copolymer solutions at different pH values, buffer concentrations and copolymer concentrations were investigated. It was found that cloud point temperature increases as a function of %EO and decreases with copolymer molecular mass, copolymer concentration and buffer concentration. The results showed that all the studied factors influenced enzyme partition. The best conditions were copolymer with 10% EO and molecular mass of 2000 g/mol, temperature of 25°C, copolymer concentration of 5% (w/w), pH 6.0 and salt concentration of 15 mM. Enzyme activity recovery around 79.5%, purification factor around 1.25 and activity partition coefficient around 1.4 were obtained. © 2004 Elsevier B.V. All rights reserved. 807 1 61 68 Brooks, D.E., Walter, H., Fisher, D., (1985) Partitioning in Aqueous Two Phase Systems, , Academic Press, Orlando Albertsson, P.A., (1986) Partition of Cell Particles and Macromolecules, Third Ed., 323p. , Wiley/Interscience, New York Walter, H., Johansson, G., (1994) Methods in Enzymology, 228, pp. 600-608. , Academic Press, Londres Alred, P.A., Tjerneld, F., Koslowski, A., Harris, J.M., (1992) Bioseparation, 2, p. 363 Franco, T.T., Galaev, Y.U., Hatti-Kaul, R., Holberg, N., Bülow, L., Mattiasson, B., (1997) Biotechnol. Tech., 11, p. 231 Persson, J., Johansson, H.-O., Tjerneld, F., (1999) J. Chromatogr. a, 864, p. 31 Persson, J., Nystrom, L., Ageland, H., Tjerneld, F., (1999) J. Chem. Technol. Biotechnol., 74, p. 238 Alexandridis, P., Hatton, T.A., (1995) Colloids Surf. A: Physicochem. Eng. Aspects, 96, p. 1 Hvidt, S., (1995) Colloids Surf. A: Physicochem. Eng. Aspects, 112, p. 201 Persson, J., Kaul, A., Tjerneld, F., (2000) J. Chromatogr. B, 743, p. 115 Berggren, K., Johansson, H.-O., Tjerneld, F., (1995) J. Chromatogr. a, 718, p. 67 Saitoh, T., Tani, H., Kamidate, T., Watanabe, H., (1995) Trends Anal. Chem., 14, p. 213 Cunha, M.T., Tjerneld, F., Cabral, J.M.S., Aires-Barros, M.R., (1998) J. Chromatogr. B, 711, p. 53 Feinstein, G., Whitaker, J.R., (1964) Biochemistry, 3, p. 1050 Murachi, T., Bromelain enzymes (1976) Methods in Enzymology, 45, pp. 475-485. , L. Lorand (Ed.), Academic Press, New York Arroyo-Reyna, A., Hernández-Arana, A., (1995) Biochim. Biophys. Acta, 1248, p. 123 Bradford, M.M., (1976) Anal. Biochem., 72, p. 248 A.C.W. Cesar, R. Silva, A.C. Lucarini, RIC, São Carlos, SP, Brazil 01 (1999) 47-54Baldini, V.L.S., Iaderoza, M., Ferreira, E.A.H., Sales, A.M., Draetta, I.S., Giacomelli, E.J., (1993) Colet. ITAL, Campinas, 23, p. 44 Karlstron, G., (1985) J. Phys. Chem., 89, p. 4962 Alred, P.A., Koslowski, A., Harris, J.M., Tjerneld, F., (1994) J. Chromatogr. a, 659, p. 289