Artículos de revistas
Patterns Of Expression Of D-mannose Receptors In Spermatozoa Of Fertile Men [padrões De Expressão Dos Receptores De D-manose Em Espermatozóides De Homens Férteis]
Registro en:
Jornal Brasileiro De Reproducao Assistida. , v. 8, n. 5, p. 9 - 15, 2004.
15175693
2-s2.0-15444366608
Autor
Silveira C.F.
Neves P.A.
Fazano F.A.T.
De Lucio M.A.
Morais S.S.
Institución
Resumen
The objective of the study was to evaluate the patterns of expression of D-mannose receptors at the surface of spermatozoa of fertile men, during capacitation and following induction of acrossomal reaction with incubation for 20 hours. Thirty patients who were candidate for vasectomy provided one semen sample before the surgery, after signing an informed consent. All men had normal general and urological physical exams and all samples met the normal criteria of the World Health Organization. All semen samples, after routine sperm analysis, were submitted to D-mannose test and to acrossomal reaction test according to the protocol proposed by Benoff et al. (1993a). All semen samples were evaluated at three different moments: after liquefaction and washing (0 hour), after swim-up (sperm processing) (1 hour), and after incubation of the samples for 20 hours, in order to induce acrossomal reaction (20 hour). Five main patterns of expression of D-mannose receptors with intact acrossome were observed. There was no statistical difference among the patterns in the three moments of observation. It was possible to establish normal patterns of expression of D-mannose receptors of normal fertile men that could be compared with semen samples from infertile men. 8 5 9 15 Benoffs, Cooper, G.W., Hurley, I., Napolitano, B., Osenfeld, D.L., Scholl, G.M., Humam sperm fertilizing potential in vitro is correlated with differential expression of a head-specific mannose - Ligand receptor (1993) Fertil Steril., 59, pp. 854-862 Benoff, S., Hurley, I., Cooper, G.W., Mandel, F.S., Hershlag, A., Scholl, G.M., Fertilization potential in vitro is correlated with headspecific mannose-ligand receptor expression, acrosome status and membrane cholesterol content (1993) Hum Reprod, 8, pp. 2155-2166 Conover, W.J., (1999) Practical Nonparametric Statistics, , New York: Wiley Flesch, F.M., Gadella, M.B., Dynamics of the mammalian sperm plasma membrane in the process of fertilization (2000) Bioch. Biophis. Acta, 1469, pp. 197-235 Gamzu, R., Yogev, L., Kleiman, S., Botchan, A., Hauser, R., Lessing, J.B., Expression of mannose-ligand receptors on human spermatozoa: Effect of lecithin and association with sperm binding to the zona pellucida (1998) Fertil. Steril., 70, pp. 766-770 Moore, K.L., Persaud, T.V.N., (2000) Embriologia Clínica, pp. 15-43. , Rio de Janeiro: Guanabara Koogan Mori, K., Daitoh, T., Irahara, M., Kamada, M., Aono, T., Significance of D-manose as a sperm receptor site on the zona pellucida in human fertilization (1989) Am J Obstet Gynecol, 161, pp. 207-211 (2001) Manual de Laboratorio de la OMS para El Examen del Semen Humano Y de la Interacción Entre El Semen Y El Moco Cervical 4a Ed., , Madrid: Editorial Médica Panamericana; Santos, J.R.F., Neves, P.A., Capacitação e reação acrossômica (2002) Infertilidade Masculina, pp. 45-48. , NEVES P., RODRIGUES NETTO JR., N. São Paulo: Atheneu Tesarik, J., Mendoza, C., Carreras, A., Expression of D-mannose binding sites on human spermatozoa: Comparison of fertile donors and infertile patients (1991) Fertil. Steril., 56, pp. 113-118 Yavetz, H., Rosenblat, Y., Yogev, L., Botchan, A., Lessing, J.B., Paz, G., Effect of freezing-thawing on the expression of mannose-ligand receptors on human spermatozoa: The impact on sperm capacitation and acrosome reaction (2001) Andrologia, 33, pp. 272-276 Youssef, H.M., Doncel, G.F., Bassiouni, B.A., Acosta, A.A., Manose binding sites on human spermatozoa and sperm morphology (1996) Fertil Steril., 66, pp. 640-645