Artículos de revistas
Mitomycin C Toxicity In The Corneal Epithelium Of Rabbits [toxicidade Da Mitomicina C No Epitélio Corneano De Coelhos]
Registro en:
Arquivos Brasileiros De Oftalmologia. , v. 67, n. 5, p. 713 - 716, 2004.
42749
2-s2.0-77952757367
Autor
Holzchuh N.
Holzchuh R.
Arieta C.E.L.
Kara-Jose N.
Alves M.R.
Institución
Resumen
Objective: To evaluate the effects of mitomycin C eye drops (0.2 mg/ml) on the corneal epithelium of rabbits. Methods: Mitomycin C and distilled water (controls) were instilled 4 times daily for 14 consecutive days in the eyes with intact ocular surface. The animals were sacrificed in the 15th, 50th and 100th day of the experiment. The histopathologic analysis of the corneal epithelium was complemented by morphometry. Epithelium area, number of nuclei, nucleus-cytoplasm relation, epithelial cell and cytoplasm area were studied. Results: The morphometric analysis was performed by point counting under light microscopy. It showed variations characterized by alteration of the epithelium area, nucleus area and cytoplasm area, epithelial cell hypertrophy, alteration of the nucleuscytoplasm relationship and decrease of the number of nuclei. Conclusion: The results of this investigation, in the study conditions, showed that 0.02% mitomycin C, instilled 4 times daily for 14 consecutive days, has low toxic potential in the intact ocular surface. 67 5 713 716 Burstein, N.L., Corneal cytotoxicity of topically applied drugs, vehicles and preservatives (1980) Surv. Ophthalmol., 25 (1), pp. 15-30 Alves, M.R., Saldiva, P.H.N., Lemos, M., Kara-José, N., Efeitos do uso tópico da mitomicina C no epitélio corneano de coelhas: Análise histopatológica pela morfometria (1996) Arq Bras Oftalmol, 59 (5), pp. 431-437 Mattar, D.B., Alves, M.R., Silva, M.H.T., Kara-José, N., Estudo da influência da aplicação subconjuntival da mitomicina C na reparação de defeito epitelial corneano em coelhas (1995) Arq Bras Oftalmol, 58 (1), pp. 65-67 Holzchuh, N., Alves, M.R., Santo, R.M., Matayoshi, S., Kara-José, N., Efeitos do uso do colírio de mitomicina C na superfície ocular de coelhos (1997) Rev Med. (São Paulo), 76 (6), pp. 303-306 Frucht-Pery, J., Rozenman, Y., Mitomycin C therapy for corneal intraepithelial neoplasia (1994) Am J Ophthalmol, 117 (2), pp. 164-168 Gundersen, H.J.G., Jensen, E.B., The efficiency of systematic sampling in stereology and its prediction (1987) J Microsc, 147 (PT. 3), pp. 229-263 Mandarim-de-Lacerda, C.A., (1995) Métodos quantitativos em morfologia, 131p. , Rio de Janeiro EDUERJ Cotran, R.S., Kumar, V., Robbins, S.L., Shoen, F.J., Cellular injury and cellular death (1994) Robbins Pathologic Basis of Disease, 1425p. , Cotran RS, Kumar V, Collins P., 6.ed. Philadelphia: Saunders Cotran, R.S., Kumar, V., Robbins, S.L., Shoen, F.J., Cellular growth and differentiation: Normal regulation and adaptations (1994) Robbins Pathologic Basis of disease, p. 1425. , Cotran RS, Kumar V, Collins P., 6.ed. Philadelphia: Saunders Crooke, S.T., Bradner, W.T., Mitomycin C: A review (1976) Cancer Treat Rev, 3 (3), pp. 121-139