Anti-sera Raised In Rabbits Against Crotoxin And Phospholipase A 2 From Crotalus Durissus Cascavella Venom Neutralize The Neurotoxicity Of The Venom And Crotoxin

dc.creatorBeghini D.G.
dc.creatorHernandez-Oliveira S.
dc.creatorRodrigues-Simioni L.
dc.creatorNovello J.C.
dc.creatorHyslop S.
dc.creatorMarangoni S.
dc.date2004
dc.date2015-06-26T14:23:14Z
dc.date2015-11-26T14:11:34Z
dc.date2015-06-26T14:23:14Z
dc.date2015-11-26T14:11:34Z
dc.date.accessioned2018-03-28T21:12:08Z
dc.date.available2018-03-28T21:12:08Z
dc.identifier
dc.identifierToxicon. , v. 44, n. 2, p. 141 - 148, 2004.
dc.identifier410101
dc.identifier10.1016/j.toxicon.2004.05.011
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-3042708770&partnerID=40&md5=4d416d2c66b7eafb462cb510b5dada46
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/94111
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/94111
dc.identifier2-s2.0-3042708770
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1241765
dc.descriptionCrotoxin, the principal neurotoxin in venom of the South American rattlesnakes Crotalus durissus terrificus and Crotalus durissus cascavella, contains a basic phospholipase A2 (PLA2) and an acidic protein, crotapotin. In this work, we examined the ability of rabbit anti-sera against crotoxin and its PLA2 subunit to neutralize the neurotoxicity of venom and crotoxin from C. d. cascavella in mouse phrenic nerve-diaphragm and chick biventer cervicis preparations. Immunoblotting showed that the anti-sera recognized C. d. cascavella crotoxin and PLA2. This was confirmed by ELISA, with both anti-sera having end-point dilutions of 3×10-6. Anti-crotoxin serum neutralized the neuromuscular blockade in phrenic nerve-diaphragm muscle preparations at venom or crotoxin:anti-serum ratios of 1:2 and 1:3, respectively. Anti-PLA2 serum also neutralized this neuromuscular activity at a venom or crotoxin:anti-serum ratio of 1:1. In biventer cervicis preparations, the corresponding ratio for anti-crotoxin serum was 1:3 for venom and crotoxin, and 1:1 and 1:2 for anti-PLA2 serum. The neutralizing capacity of the sera in mouse preparations was comparable to that of commercial anti-serum raised against C. d. terrificus venom. These results show that anti-sera against crotoxin and PLA2 from C. d. cascavella venom neutralized the neuromuscular blockade induced by venom and crotoxin in both nerve-muscle preparations, with the anti-serum against crotoxin being slightly less potent than that against crotoxin. © 2004 Elsevier Ltd. All rights reserved.
dc.description44
dc.description2
dc.description141
dc.description148
dc.descriptionAzevedo-Marques, M.M., Cupo, P., Coimbra, T.M., Hering, S.E., Rossi, M.A., Laure, C.J., Myonecrosis, myoglobinuria and acute renal failure induced by South-American rattlesnake (Crotalus durissus terrificus) envenomation in Brazil (1982) Toxicon, 23, pp. 631-636
dc.descriptionBeghini, D.G., Toyama, M.H., Hyslop, S., Sodek, L., Novello, J.C., Marangoni, S., Enzymatic characterization of a novel phospholipase A2 from Crotalus durissus cascavella rattlesnake (maracambóia) venom (2000) J. Protein Chem., 19, pp. 603-607
dc.descriptionBon, C., Changeux, J.P., Jeng, T.W., Fraenkel-Conrat, H., Postsynaptic effects of crotoxin and of its isolated subunits (1979) Eur. J. Biochem., 99, pp. 471-481
dc.descriptionBreithaupt, H., Enzymatic characteristics of Crotalus phospholipase A2 and the crotoxin complex (1976) Toxicon, 14, pp. 221-233
dc.descriptionBülbring, E., Observation on the isolated phrenic nerve diaphragm preparation of the rat (1946) Br. J. Pharmacol., 1, pp. 38-61
dc.descriptionCamara, P.R., Esquisatto, L.C., Camargo, E.A., Ribela, M.T., Toyama, M.H., Marangoni, S., De Nucci, G., Antunes, E., Inflammatory oedema induced by phospholipases A2 isolated from Crotalus durissus sp. in the rat dorsal skin: A role for mast cells and sensory C-fibers (2003) Toxicon, 41, pp. 823-829
dc.descriptionChavez-Olórtegui, C., Silva Lopes, C., Drumond Cordeiro, F., Granier, C., Diniz, C.R., An enzyme linked immunosorbent assay (ELISA) that discriminates between Bothrops atrox and Lachesis muta muta venoms (1993) Toxicon, 31, pp. 417-425
dc.descriptionChoumet, V., Jiang, M.S., Radvanyi, F., Ownby, C., Bon, C., Neutralization of lethal potency and inhibition of enzymatic activity of a phospholipase A2 neurotoxin, crotoxin, by non-precipitating antibodies (Fab) (1989) FEBS Lett., 244, pp. 167-173
dc.descriptionChoumet, V., Faure, G., Robbe-Vincent, A., Saliou, B., Mazié, J.C., Bon, C., Immunochemical analysis of a snake venom phospholipase A2 neurotoxin, crotoxin, with monoclonal antibodies (1992) Mol. Immunol., 29, pp. 871-882
dc.descriptionChoumet, V., Lafaye, P., Mazié, J.C., Bon, C., A monoclonal antibody directed against the non-toxic subunit of a dimeric phospholipase A2 neurotoxin, crotoxin, neutralizes its toxicity (1998) J. Biol. Chem., 379, pp. 899-906
dc.descriptionDos Santos, M.C., Diniz, C.R., Pacheco, M.A., Dias Da Silva, W., Phospholipase A2 injection in mice induces immunity against the lethal effects of Crotalus durissus terrificus venom (1988) Toxicon, 26, pp. 207-213
dc.descriptionFaure, G., Harvey, A.L., Thomson, E., Saliou, B., Radvani, F., Bon, C., Comparison of crotoxin isoforms reveals that stability of the complex plays a major role in its pharmacological action (1993) Eur. J. Biochem., 214, pp. 491-496
dc.descriptionFreitas, T.V., Fortes-Dias, C.L., Diniz, C.R., Protection against the lethal effects of Crotalus durissus terrificus (South American rattlesnake) venom in animals immunized with crotoxin (1990) Toxicon, 28, pp. 1491-1496
dc.descriptionGinsborg, B.L., Warriner, J., The isolated chick biventer cervicis nerve muscle preparation (1960) Br. J. Pharmacol., 15, pp. 410-411
dc.descriptionHendon, R.A., Fraenkel-Conrat, H., Biological roles of the two components of crotoxin (1971) Proc. Natl Acad. Sci. USA, 68, pp. 1560-1563
dc.descriptionJeng, T.W., Hendon, R.A., Fraenkel-Conrat, H., Search for relationship among the hemolytic, phospholipolytic and neurotoxic activities of snake venoms (1978) Proc. Natl Acad. Sci. USA, 75, pp. 600-604
dc.descriptionLanducci, E.C., Condino-Neto, A., Perez, A.C., Hyslop, S., Corrado, A.P., Novello, J.C., Marangoni, S., De Nucci, G., Crotoxin induces aggregation of human washed platelets (1994) Toxicon, 32, pp. 217-226
dc.descriptionLomonte, B., Gutiérrez, J.M., Moreno, E., Cerdas, L., Antibody neutralization of a myotoxin from the venom of Bothrops asper (terciopelo) (1987) Toxicon, 25, pp. 443-449
dc.descriptionMarlas, G., Purification and preliminary structure of a potent platelet aggregating glycoprotein isolated from the venom of Crotalus durissus cascavella (1982) Toxicon, 20, pp. 289-290
dc.descriptionMarlas, G., Isolation and characterization of the alpha and beta subunits of the platelet-activating glycoprotein from the venom of Crotalus durissus cascavella (1985) Biochimie, 67, pp. 1232-1239
dc.descriptionMarlas, G., Bon, C., Relationship between the pharmacological action of crotoxin and its phospholipase activity (1982) Eur. J. Biochem., 125, pp. 157-165
dc.descriptionMarlas, G., Joseph, D., Huet, C., I-isolation and electron microscope studies of a potent platelet-aggregating glycoprotein from the venom of Crotalus durissus cascavella (1983) Biochimie, 65, pp. 405-416
dc.descriptionMarlas, G., Joseph, D., Huet, C., Subunit structure of a potent platelet-activating glycoprotein isolated from the venom of Crotalus durissus cascavella (1983) Biochimie, 65, pp. 619-628
dc.descriptionMartins, A.M., Monteiro, H.S., Júnior, E.O., Menezes, D.B., Fonteles, M.C., Effects of Crotalus durissus cascavella venom in the isolated rat kidney (1998) Toxicon, 36, pp. 1441-1450
dc.descriptionMartins, A.M., Toyama, M.H., Havt, A., Novello, J.C., Marangoni, S., Fonteles, M.C., Monteiro, H.S., Determination of Crotalus durissus cascavella venom components that induce renal toxicity in isolated rat kidneys (2002) Toxicon, 40, pp. 1165-1171
dc.descriptionMartins, A.M., Lima, A.A., Toyama, M.H., Marangoni, S., Fonteles, M.C., Monteiro, H.S., Renal effects of supernatant from macrophages activated by Crotalus durissus cascavella venom: The role of phospholipase A2 and cyclooxygenase (2003) Pharmacol. Toxicol., 92, pp. 14-20
dc.descriptionOliveira, D.G., Toyama, M.H., Martins, A.M., Havt, A., Nobre, A.C., Marangoni, S., Câmara, P.R., Monteiro, H.S., Structural and biological characterization of a crotapotin isoform isolated from Crotalus durissus cascavella venom (2003) Toxicon, 42, pp. 53-62
dc.descriptionOshima-Franco, Y., Hyslop, S., Prado-Franceschi, J., Cruz-Höfling, M.A., Rodrigues-Simioni, L., Neutralizing capacity of antisera raised in horses and rabbits against Crotalus durissus terrificus (South American rattlesnake) venom and its main toxin, crotoxin (1999) Toxicon, 37, pp. 1341-1457
dc.descriptionOuchterlony, O., Antigen-antibody reactions in gels (1949) Acta Path. Microbiol. Scand., 26, pp. 507-515
dc.descriptionRosenfeld, G., Symptomatology, pathology and treatment of snake bites in South America (1971) Venomous Animals and Their Venoms, 2, pp. 345-384. , W. Bücherl, Buckley E.E. New York: Academic Press
dc.descriptionSantoro, M.L., Sousa-E-Silva, M.C.C., Gonçalvez, L.R.C., Almeida-Santos, S.M., Cardoso, D.F., Laporta-Ferreira, I.L., Saiki, M., Sano-Martins, I.S., Comparison of the biological activities in venoms from three subspecies of the South American rattlesnake (Crotalus durissus terrificus, C. durissus cascavella and C. durissus collilineatus) (1999) Comp. Biochem. Physiol. Part C, 122, pp. 61-73
dc.descriptionSlotta, K.H., Fraenkel-Conrat, H.L., Schlangengifte, III: Mitteilung Reiningung und Krystallization des Klapperschangengiftes (1938) Ber. Dich. Chem. Ges., 71, pp. 1076-1081
dc.descriptionVital Brazil, O., Pharmacology of crystalline crotoxin. II. Neuromuscular blocking action (1966) Mem. Inst. Butantan, 33, pp. 981-992
dc.languageen
dc.publisher
dc.relationToxicon
dc.rightsfechado
dc.sourceScopus
dc.titleAnti-sera Raised In Rabbits Against Crotoxin And Phospholipase A 2 From Crotalus Durissus Cascavella Venom Neutralize The Neurotoxicity Of The Venom And Crotoxin
dc.typeArtículos de revistas


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