Artículos de revistas
Hplc Analysis Of Carotenoids From Five Rhodotorula Strains [análise, Por Clae, De Carotenóides De Cinco Linhagens De Rhodotorula]
Registro en:
Revista Brasileira De Ciencias Farmaceuticas/brazilian Journal Of Pharmaceutical Sciences. , v. 39, n. 3, p. 309 - 318, 2003.
15169332
2-s2.0-0345283059
Autor
Squina F.M.
Mercadante A.Z.
Institución
Resumen
A method for extraction and HPLC separation of carotenoids from five Rhodotorula strains was optimized. The extraction by mechanical disruption of the yeast cell wall with fine treated sand was shown to be more efficient than chemical disruption with dimethylsulfoxide. The carotenoids were separated and quantified by HPLC on a C 18 column using as mobile phase acetonitrile/methanol (0.1% triethylamina)/ethyl acetate (75:15:10) with 100% methanol (0.1% triethylamine) between the injections, at a flow rate of 1.0 mL/min. In all strains, the major carotenoids found were torularhodin, torulene, γ-carotene and β-carotene. The total carotenoid contents, in μg/g, obtained were 251.7 for R. glutinis, 123.5 for R. rabra, 113.2 for R. araucariae, 105.8 for R. lactosa and 103.7 for R. minuta. 39 3 309 318 Assunção, R.B., Mercadante, A.Z., Carotenoids and ascorbic acid from cashew apple (Anacardium occidentale L) (2003) Food Chem., 81, pp. 495-502. , Amsterdam Britton, G., Overview of carotenoid biosynthesis (1998) Synthesis and Metabolism, 3, pp. 13-147. , Britton, G. Liaaen-Jensen, S. Pfander, H., eds. Carotenoids: bio. Basel: Birkhäuser Verlag Britton, G., UV/Visible spectroscopy (1995) Carotenoids: Spectroscopy, 1 B, pp. 13-62. , Britton, G. Liaaen-Jensen, S. Pfander, H., eds. Basel: Birkhäuser Verlag Bushway, R.J., Wilson, A.M., Determination of α- and β-carotene in fruit and vegetables by high performance liquid chromatography (1982) Can. Inst. Food. Sci. Technol. J., 15, pp. 165-169. , Ottawa Buzzini, P., Martini, A., Production of carotenoids by strains of Rhodotorula glutinis cultured in raw materials of agro-industrial origin (1999) Bioresour. Technol., 71, pp. 41-44. , Amsterdam Davies, B.H., Carotenoids (1976) Chemistry and Biochemistry of Plant Pigments. 2. Ed., 2, pp. 38-165. , Goodwin, T. W., ed. London: Academic Press Frengova, G., Simova, E., Pavalova, K., Beshkova, D., Grigorova, D., Formation of carotenoids by Rhodotorula glutinis in whey ultrafiltrate (1994) Biotechnol. Bioeng., 44, pp. 888-894. , New York Frengova, G.I., Simova, E.D., Beshkova, D.M., Effect of temperature changes on the production of yeast pigments co-cultivated with lacto-acid bacteria in whey ultrafiltrate (1995) Biotechnol. Lett., 17, pp. 1001-1006. , Dordrecht Haard, N.F., Astaxanthin formation by the yeast Phaffia rhodozyma on molasses (1988) Biotechnol. Lett., 10, pp. 609-614. , Dordrecht Hamano, P.S., Mercadante, A.Z., Composition of carotenoids from commercial products of caja (Spondias luteas) (2001) J. Food Compos. Anal., 14, pp. 335-343. , Orlando Hayman, E.P., Yokoyama, H., Chichester, C., Simpson, K.L., Carotenoid biosynthesis in Rhodotorula glutinis (1974) J. Bacteriol., 120, pp. 1339-1343. , Washington Johnson, E.A., Schroeder, W.A., Microbial carotenoids (1995) Adv. Biochem. Eng./Biotechnol., 53, pp. 119-178. , Berlin Johnson, E.A., Villa, T.G., Lewis, M.J., Phaff, H.J., Simple method for the isolation of astaxanthin from the Basidiomycetous yeast Phaffia rhodozyma (1978) Appl. Environ. Microbiol., 35, pp. 1155-1158. , Washington Kester, A.S., Computer-optimized normal-phase high-performance liquid chromatographic separation of corynebacterium poinsettiae carotenoids (1984) J. Chromatogr., 310, pp. 372-378. , Amsterdam Krinsky, N.I., The biological properties of carotenoids (1994) Pure Appl. Chem., 66, pp. 1003-1010. , Research Triangle Park Martelli, H., Silva, I.M., Beta-carotene synthesis in Rhodotorula (1993) Methods Enzymol., 214, pp. 386-390. , San Diego Martelli, H.L., Silva, I.M., Souza, N.O., Pomeroy, D., Production of β-carotene by a Rhodotorula strain grown on sugar cane juice (1990) Biotechnol. Lett., 12, pp. 207-208. , Dordrecht Martin, A.M., Lu, C., Patel, T.R., Growth parameters for the yeast Rhodotorula rubra grown in peat extracts (1993) J. Ferment. Bioeng., 76, pp. 321-325. , Amsterdam Meyers, S.P., Developments in world aquaculture, feed formulation, and role of carotenoids (1994) Pure Appl. Chem., 66, pp. 1069-1976. , Research Triangle Park Nam, H.S., Chi, S.Y., Rhee, J.S., High-performance liquid chromatographic analysis of major carotenoids from Rhodotorula glutinis (1988) J. Chromatogr., 448, pp. 445-447. , Amsterdam Okagbue, R.N., Lewis, M.J., Autolysis of the red yeast Phaffia rhodozyma: A potential tool to facilitate extraction of astaxanthin (1984) Biotechnol. Lett., 6, pp. 247-250. , Dordrecht Parajó, J.C., Santos, V., Vazquez, M., Optimization of carotenoid production by Phaffia rhodozyma cells grown on xylose (1998) Process Biochem., 33, pp. 181-187. , Oxford Perrier, V., Dubreucq, E., Gayzy, P., Fatty acid and carotenoid composition of Rhodotorula strains (1995) Arch. Microbiol., 164, pp. 173-179. , Berlin Peterson, W.J., Lecce, E.E., Bell, T.A., Etchells, J.L., Quantitative determination of the carotenoids in yeasts of the genus Rhodotorula (1958) J. Bacteriol., 75, pp. 586-591. , Washington Scott, K.J., Finglas, P.M., Seale, R., Hart, D.J., Froidmont-Görtz, I., Interlaboratory studies of HPLC procedures for the analysis of carotenoids in foods (1996) Food Chem., 57, pp. 85-90. , Amsterdam Sedmak, J.J., Weerasinghe, D.K., Jolly, S.O., Extraction and quantitation of astaxanthin from Phaffia Rhodozyma (1990) Biotechnol. Tech., 4, pp. 107-112. , London Shih, C.T., Hang, Y.D., Production of carotenoids by Rhodotorula rubra from sauerkraut brine (1996) Lebensm.-Wiss. Technol., 29, pp. 570-572. , London Simpson, K.L., Nakayama, T.O.M., Chichester, C.O., Biosynthesis of yeast carotenoids (1964) J. Bacteriol., 88, pp. 1688-1094. , Washington Squina, F.M., Yamashita, F., Pereira, J.L., Mercadante, A.Z., Production of carotenoids by Rhodotorula rubra and R. glutinis in culture medium supplemented with sugar cane juice (2002) Food Biotechnol., 16, pp. 227-235. , New York