Actas de congresos
New Stationary Phase For Anion-exchange Chromatography
Registro en:
Journal Of Chromatography A. , v. 1073, n. 1-2, p. 147 - 153, 2005.
219673
10.1016/j.chroma.2004.10.012
2-s2.0-17444407530
Autor
Auler L.M.L.A.
Silva C.R.
Collins K.E.
Collins C.H.
Institución
Resumen
This work describes the preparation of an anion-exchange phase based on silica, using a two-step modification process. First, 10 μm Davisil silica particles were silanized with chloropropyltrimethoxysilane to yield chloropropyl silica. The modified silica was then reacted with pyridine to produce positively charged propylpyridinium groups on the surface, the anion-exchange sites. The phase was characterized by thermogravimetric analysis and infrared and solid state 13C and 29Si NMR spectroscopies. HPLC separations of common inorganic anions, including chloride, nitrite, bromide and nitrate, were performed using 150 × 3.9 HPLC columns packed with the phase, using a phthalate buffer solution as mobile phase with non-suppressed conductivity detection. Efficiency and resolution were calculated and the results show that the new phase has significant promise for the analysis of these anions in environmental samples. © 2004 Elsevier B.V. All rights reserved. 1073 1-2 147 153 Small, H., Stevens, T.S., Bauman, W.C., (1975) Anal. Chem., 47, p. 1801 Woodruff, A., Pohl, C.A., Bordunov, A., Avdalovic, N., (2003) J. Chromatogr. a, 997, p. 33 Jal, P.K., Patel, S., Mishra, B.K., (2004) Talanta, 62, p. 1005 Collinson, M.M., (2002) Trends Anal. Chem., 21, p. 35 Sugrue, E., Nesterenko, P., Paull, B., (2004) J. Sep. Sci., 27, p. 921 Sarzanini, C., (2002) J. Chromatogr. a, 956, p. 3 Cortes, H.J., (1982) J. Chromatogr., 234, p. 517 Kiseleva, M.G., Nestenko, P.N., (2001) J. Chromatogr. a, 920, p. 87 Connolly, D., Paull, B., (2001) J. Chromatogr. a, 917, p. 353 Hatsis, P., Lucy, C.A., (2002) Analyst, 127, p. 451 Hatsis, P., Lucy, C.A., (2003) Anal. Chem., 75, p. 995 Connolly, D., Victory, D., Paull, B., (2004) J. Sep. Sci., 27, p. 912 Tundo, P., Venturello, P., Angeletti, E., (1982) J. Am. Chem. Soc., 104, p. 6547 Fujiwara, S.T., Alfaya, R.V.S., Gushikem, Y., (2001) Colloid Surf. A: Physicochem. Eng. Aspects, 178, p. 135 Iamamoto, M.S., Gushikem, Y., (1989) Analyst, 114, p. 983 Engelhardt, H., Orth, P., (1987) J. Liq. Chromatogr. Relat. Technol., 10, p. 1999 Collins, K.E., Franchon, A.C., Jardim, I.C.S.F., Radovanovic, E., Gonçalves, M.C., (2000) LC-GC, 18, p. 106 Neue, U.D., (1997) HPLC Columns, Theory, Technology and Practice, , Wiley-VCH New York Silverstein, R.M., Basser, G.C., Morrill, T.C., (1991) Spectrometric Identification of Organic Compounds, , second ed. John Wiley & Sons Inc. New York Pavia, D.L., Lampman, G.M., Kriz, G.S., (1996) Introduction to Spectroscopy, , second ed. Saunders College Publishing New York Bachmann, S., Melo, L.F.C., Silva, R.B., Anazawa, T.A., Jardim, I.C.S.F., Collins, K.E., Collins, C.H., Albert, K., (2001) Chem. Mater., 13, p. 1874 Albert, K., Bayer, E., (1991) J. Chromatogr., 544, p. 345 Lumley, B., Khong, T.M., Perrett, D., (2004) Chromatographia, 60, p. 59 Claessens, H.A., Van Straten, M.A., Kirkland, J.J., (1996) J. Chromatogr. a, 728, p. 259