Artículos de revistas
Biochemical, Pharmacological And Structural Characterization Of A New Pla2 From Crotalus Durissus Terrificus (south American Rattlesnake) Venom
Registration in:
Protein Journal. , v. 24, n. 4, p. 233 - 242, 2005.
15723887
10.1007/s10930-005-6718-z
2-s2.0-27744443755
Author
Hernandez-Oliveira S.
Toyama M.H.
Toyama D.O.
Marangoni S.
Hyslop S.
Rodrigues-Simioni L.
Institutions
Abstract
A new PLA2 (F16) was purified from Crotalus durissus terrificus venom by molecular exclusion chromatography followed by analytical reverse phase HPLC. The PLA2 (14.86 kDa by MALDI-TOF mass spectrometry) had an amino acid sequence of SLLQFNKMIKFETRKNAVPFYAFYGCYCGWGGRRRPKDATDRCCFVHDCCYEKVTKCNTKWD IYRYSLKSGYITCGKGTWCKEQICECDRVAAECLRRSLSTYKNGYMFYPDSRCRGPSETC, and showed highly conserved Ca2+-binding and catalytic sites. F16 showed allosteric behavior with 10 mM Ca2+ and had temperature and pH optima of 25°C and 7.9, respectively. F16 (10 μg/ml) produced neuromuscular blockade in chick biventer cervicis preparations in the absence and presence of crotapotin, indicating that crotapotin was not essential for neuromuscular action in this preparation. In contrast, in mouse phrenic nerve-diaphragm preparations, the neuromuscular blockade produced by the same concentration of toxin was dependent on crotapotin. Pre-incubation with heparin markedly reduced the neurotoxicity of F16. These results show that the biochemical and structural properties of F16 are similar to those of the PLA2 isoforms F15 and F17, but that the neurotoxicity and the requirement for crotapotin to form the crotoxin complex varies according to the neuromuscular preparation. © 2005 Springer Science+Business Media, Inc. 24 4 233 242 Araújo, F.A.A., Santalúcia, M., Cabral, R.F., Cardoso, J.L.C., França, F.O.S., Fan, H.W., Málaque, C.M.S., Haddad Jr., V., Epidemioligia dos Acidentes por Animais Peçonhentos. (2003) Animais Peçonhentos No Brasil. Biologia, Clínica e Terapêutica Dos Acidentes, pp. 6-12. , Sarvier/FAPESP São Paulo Azevedo-Marques, M.M., Hering, S.E., Cupo, P., Cardoso, J.L.C., França, F.O.S., Fan, H.W., Málaque, C.M.S., Haddad Jr., V., Acidente Crotálico. (2003) Animais Peçonhentos No Brasil. Biologia, Clínica e Terapêutica Dos Acidentes, pp. 91-98. , Sarvier/FAPESP São Paulo Beghini, D.G., Hernandez-Oliveira, S., Rodrigues-Simioni, L., Novello, J.C., Hyslop, S., Marangoni, S., (2004) Toxicon, 44, pp. 141-148 Beghini, D.G., Rodrigues-Simioni, L., Toyama, M.H., Novello, J.C., Da Cruz-Höfling, M.A., Marangoni, S., (2004) Toxicon, 43, pp. 255-261 Beghini, D.G., Toyama, M.H., Hyslop, S., Sodek, L., Novello, J.C., Marangoni, S., (2000) J. Protein Chem., 19, pp. 603-607 Bon, C., Bouchier, C., Choumet, V., Faure, G., Jiang, M.S., Lambezat, M.P., Radvanyi, F., Saliou, B., (1989) Acta Physiol. Pharmacol. Latinoam., 39, pp. 439-448 Bonventre, J.V., Sapirstein, A., (2002) Adv. Exp. Med. Biol., 507, pp. 25-31 Bülbring, E., (1946) Br. J. Pharmacol., 1, pp. 38-61 Choumet, V., Bouchier, C., Delot, E., Faure, G., Saliou, B., Bon, C., (1996) Adv. Exp. Med. Biol., 391, pp. 197-202 Dennis, E.A., (1994) J. Biol. Chem., 269, pp. 13057-13060 Faure, G., Bon, C., (1987) Toxicon, 25, pp. 229-234 Faure, G., Bon, C., (1988) Biochemistry, 27, pp. 730-738 Faure, G., Choumet, V., Bouchier, C., Camoin, L., Guillaume, J.L., Monegier, B., Vuilhorgne, M., Bon, C., (1994) Eur. J. Biochem., 223, pp. 161-164 Faure, G., Guillaume, J.L., Camoin, L., Saliou, B., Bon, C., (1991) Biochemistry, 30, pp. 8074-8083 Faure, G., Harvey, A.L., Thomson, E., Saliou, B., Radvanyi, F., Bon, C., (1993) Eur. J. Biochem., 214, pp. 491-496 Ginsborg, B.L., Warriner, J., (1960) Br. J. Pharmacol., 15, pp. 410-421 Gutiérrez, J.M., Lomonte, B., (1995) Toxicon, 33, pp. 1405-1424 Gutiérrez, J.M., Ownby, C.L., (2003) Toxicon, 42, pp. 915-931 Habermann, E., Breithaupt, H., (1978) Toxicon, 16, pp. 19-30 Hendon, R.A., Fraenkel-Conrat, H., (1971) Proc. Natl. Acad. Sci. USA, 68, pp. 1560-1563 Holzer, M., MacKessy, S.P., (1996) Toxicon, 34, pp. 1149-1155 Kini, R.M., (2003) Toxicon, 42, pp. 827-840 Kini, R.M., Evans, H.J., (1989) Toxicon, 7, pp. 613-635 Kudo, I., Murakami, M., (2002) Prostag. Oth. Lipid Mediat., 68, pp. 3-58 Lambeau, G., Lazdunski, M., (1999) Trends Pharmacol. Sci., 20, pp. 162-170 Lennon, B.W., Kaiser, I.I., (1990) Comp. Biochem. Physiol. B, 97, pp. 695-699 Lomonte, B., Angulo, Y., Calderón, L., (2003) Toxicon, 42, pp. 885-901 Mayer, R.J., Marshall, L.A., (1993) FASEB J., 7, pp. 339-348 Oliveira, D.G., Toyama, M.H., Novello, J.C., Beriam, L.O., Marangoni, S., (2002) J. Protein Chem., 21, pp. 161-168 Ponce-Soto, L.A., Toyama, M.H., Hyslop, S., Novello, J.C., Marangoni, S., (2002) J. Protein Chem., 21, pp. 131-136 Rangel-Santos, A., Dos-Santos, E.C., Lopes-Ferreira, M., Lima, C., Cardoso, D.F., Mota, I., (2004) Toxicon, 43, pp. 801-810 Selistre-De-Araujo, H., White, S.P., Ownby, C.L., (1996) Arch. Biochem. Biophys., 326, pp. 21-30 Soares, A.M., Giglio, J.R., (2003) Toxicon, 42, pp. 855-868 Toyama, M.H., Oliveira, D.G., Beriam, L.O.S., Novello, J.C., Rodrigues-Simioni, L., Marangoni, S., (2003) Toxicon, 41, pp. 1033-1045 Toyama, M.H., Soares, A.M., Vieira, C.A., Novello, J.C., Oliveira, B., Giglio, J.R., Marangoni, S., (1998) J. Protein Chem., 17, pp. 713-718 Toyama, M.H., Soares, A.M., Wen-Hwa, L., Polikarpov, I., Giglio, J.R., Marangoni, S., (2000) Biochimie, 82, pp. 245-250