Actas de congresos
Non-linear Micro-spectroscopy In An Optical Tweezers System: Application To Cells Marked With Quantum Dots
Registro en:
Progress In Biomedical Optics And Imaging - Proceedings Of Spie. , v. 5700, n. , p. 273 - 278, 2005.
16057422
10.1117/12.586433
2-s2.0-21844468960
Autor
Fontes A.
De Thomaz A.A.
Moreira W.L.
Neves A.A.R.
Barbosa L.C.
De Farias P.M.A.
Santos B.S.
Cesar C.L.
Institución
Resumen
In this work we used our set up consisting of an optical tweezers plus non-linear micro-spectroscopy system to perform scanning microscopy and observe spectra using two photon excited (TPE) luminescence of captured single cells conjugated with quantum dots of CdS and CdTe. The CdS nanocrystals are obtained by our group via colloidal synthesis in aqueous medium with final pH = 7 using sodium polyphosphate as the stabilizing agent. In a second step the surface of CdS particles is functionalized with linking agents such as Glutaraldehyde. The CdTe quantum dots are functionalized in the its proper synthesis using mercaptoacetic acid (AMA). We used a femtosecond Ti:sapphire laser to excite the hyper Rayleigh or TPE luminescence in particles trapped with an Nd:YAG cw laser and a 30 cm monochromator equipped with a cooled back illuminated CCD to select the spectral region for imaging. With this system we obtained hyper Rayleigh and TPE luminescence images of macrophages and other samples. The results obtained show the potential presented by this system and fluorescent labels to perform spectroscopy in a living trapped microorganism in any neighbourhood and dynamically observe the chemical reactions changes in real time. 5700
273 278 Ashkin, A., Dziedzic, J.M., Optical trapping and manipulation of viruses and bacteria (1987) Science, 235, pp. 1517-1520 Sakata-Sogawa, K., Direct measurement of DNA molecular length in solution using optical tweezers: Detection of looping due to binding protein interactions (1998) Eur. J Biophy, 27, pp. 55-61 Konig, K., Determination of motility forces of human spermatozoa using an 800nm optical trap (1996) Cell. Mol. Biol., 42, pp. 501-509 Konig, K., Laser tweezers and multiphoton microscopes in life sciences (2000) Hyst. and Cell Biol., 114, pp. 79-91 Borchert, H., Talapin, D.V., McGinley, C., High resolution photoemission study of CdSe and CdSe/ZnS core-shell nanocrystals (2003) J. Chem. Phys., 271, pp. 1800-1807 Alivisatos, A.P., Semiconductor clusters, nanocrystals, and quantum dots (1996) Science, 271, pp. 933-937 Nowak, C., Döllefeld, H., Eychmüller, A., Innershell absorption spectroscopy on CdS: Free clusters and nanocrystals (2001) J. Chem. Phys., 114, pp. 489-494 Eychmuller, A., Structure and photophysics of semiconductor nanocrystals (1996) J. Phys Chem. B., 59, pp. 13226-13239 Bruchez Jr., M., Moronne, M., Gin, P., Weiss, S., Alivisatos, A.P., Semiconductor nanocrystals as fluorescent biological labels (1998) Science, 281, pp. 2013-2016 Chan, W.C.W., Nie, S., Quantum dots for ultrasensitive biological detection (1998) Science, 281, pp. 2016-2018 Ballou, B., Lagerholm, B.C., Ernst, L.A., Bruchez, M.P., Waggoner, A.S., Noninvasive imaging of quantum dots in mice (2004) Bioconjug. Chem., 15, pp. 79-86 Gao, X., Nie, S., Molecular profiling of single cells and tissue specimens with quantum dots (2003) Trends Biotechnol., 21, pp. 371-373 Äkerman, M.E., Chan, W.C.W., Laakkonen, P., Bhatia, S.N., Ruoslahti, E., Nanocrystal targeting in vivo (2002) Appl. Biol. Sci., 99, pp. 12617-12621 Chan, W.C.W., Maxwell, D.J., Gao, X., Bailey, R.E., Han, M., Shuming, N., Luminescent quantum dots for multiplexed biological detection and imaging (2002) Curr. Op. Bioteh., 13, pp. 40-46 Mattheakis, L.C., Dias, J.M., Choi, Y., Gong, J., Bruchez, M.P., Liu, J., Wang, E., Optical coding of mammalian cells using semiconductor quantum dot (2004) Anal Biochem., 327, pp. 200-208 Petrov, D.V., Santos, B.S., Pereira, G.A.L., Donegá, C.M., Size and band-gap dependences of the hiperpolarizability of CdxZn1-xS nanocrystals (2002) J. Phys. Chem. B, 106, pp. 5325-5334 Ajito, K., Torimitsu, K., Single nanoparticle trapping using a Raman tweezers microscope (2002) Applied Spectroscopy, 56, pp. 541-544 Xie, C.G., Dinno, M.A., Li, Y.Q., Near-infrared Raman spectroscopy of single optically trapped biological cells (2002) Optics Letters, 27, pp. 249-251 Wood, B.R., Tait, B., McNaughton, D., Micro-Raman characterisation of the R to T State transition of haemoglobin within a single living erythrocyte (2001) Bioch. et Biophys. Acta, 1539, pp. 58-70