Artículos de revistas
Obtention And Chemical And Nutritional Characterization Of Different Bovine Casein Concentrates [obtenção E Caracterização Química E Nutricional De Diferentes Concentrados De Caseína]
Registro en:
Revista De Nutricao. , v. 18, n. 1, p. 75 - 83, 2005.
14155273
2-s2.0-17844386877
Autor
Roman J.A.
Sgarbieri V.C.
Institución
Resumen
Objetive: The objective of the present work was to evaluate the centesimal composition, the amino acid profile and the the composition and nutritive value of three casein concentrates, obtained by different methods. Methods: The casein concentrates were analysed according to the following methods: a commercial casein obtained by acid precipitation (pl) followed by neutralization; an enzyme coagulated casein obtained by rennet coagulation; and a casein micelles concentrate obtained by microlfiltration and diafiltration. The Centesimal composition was determined by the procedures described in the manual Official Methods of Analysis. The amino acid profiles were determined after the protein acid hydrolysis (HCl 6N, 105°C, 22h) in an amino acid autoanalyzer by cation exchange chromatography and post-column ninhydrin reaction. The essential amino acids were in accordance with Food and Agriculture Organization/World Health Organization recommendation for children 2 to 5 years of age. The nutritive value was determined on 21-day old rats of the Wistar strain through apparent net protein ratio, and protein efficiency ratio operational. Results: Commercial casein presented higher protein concentration (92.0%) than micellar casein (86.0%) and casein clot (72.0%). The animals in the various protein diets did not show significant differences as to body weight gain and diet consumption. The highest digestibility was that of commercial casein (93.8%), compared to the other two concentrates (91.0%). Conclusion: The casein concentrates showed differences related to the centesimal composition, since the commercial casein presented a higher protein concentration. The casein clot was inferior to the other protein concentrates regarding net protein ratio and protein efficiency ratio operational. 18 1 75 83 Sgarbieri, V.C., (1996) Proteínas Em Alimentos Protéicos: Propriedades, Degradações, Modificações, , São Paulo: Varela Davian, C., Farnelart, M.H., Pierre, A., Gouderanche, H., Maubois, J.L., Rennet coagulation of skin milk and curd drainage: Effect of pH, casein concentration, ionic strength and heat treatment (2000) Le Lait, 80 (4), pp. 397-415 Gaucheron, F., Le Graët, Y., Briard, V., Effect of sodium chloride addition on the mineral equilibrium of concentrated and acidified casein micelles (2000) Milchwissenschaft, 55 (2), pp. 82-86 Wong, D.W.S., Camirant, W.M., Pavlath, A.E., Structures and functionalities of milk proteins (1996) Crit Rev Food Sci Nutr, 36 (8), pp. 807-844 Southward, C.R., Utilization of milk components (1986) Modern Dairy Technology - Advances in Milk Processing, pp. 317-368. , New York: Elselvier Fox, P.F., The milk protein system (1989) Developments in Dairy Chemistry - 4, pp. 1-55. , Fox PF, editor. London: Elsevier Kinsella, J.E., Milk proteins: Physico-chemical and functional properties (1984) Rev Food Sci Nutr, 21 (2), pp. 197-262 Giese, J., Proteins as ingredients: Types, functions, applications (1994) Food Technol, 48 (10), pp. 50-60 Southward, C.R., Use of casein and caseinates (1989) Development in Dairy Chemistry, pp. 173-224. , Fox PF, editor. New York: Elselvier Torres, J.A., Addible films and coating from proteins (1994) Protein Functionality in Food Systems, pp. 467-507. , Hettiarachechy NS, Ziegler GR, editors. New York: Marcel Dekker Smithers, G., Value added milk protein products (1991) Austr Biotechnol, 1 (1), pp. 36-39 Punidadas, P., Rizvl, S.S.H., Separation of milk proteins into fractions rich in casein or whey proteins by cross flow filtration (1998) Food Res Inter, 31 (4), pp. 265-272 Ferreira, C.V., (2001) Influência Da Velocidade Tangencial e Da Pressão Transmembrana Na Obtenção de Caseína Por Microfiltração, , [dissertação]. Campinas: Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas Osterland, N., New developments in membrane processing (1988) Proceedings of the 25th International Dairy Congress, pp. 30-31. , Sep 21-24, Aarhus, Denmark, International Dairy Federation Boland, M., MacGibbon, A., Hill, J.P., Designer milks for the new millennium (2001) Livestock Prod Sci, 56, pp. 117-122 Creamer, L.K., Pearce, L.E., Hill, J.P., Roland, M.J., Milk and dairy products in the 21st century (2002) J Agric Food Chem, 50 (25), pp. 7187-7193 (1990) Official Methods of Analysis. 15th Ed., , Washington, DC Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification (1959) Can Biochem Physiol, 37 (8), pp. 911-917 (1995) Official Methods of Analysis. 16th Ed., , Washington, DC Spies, J.R., Determination of tryptofan in proteins (1967) Analyt Chem, 39 (12), pp. 1412-1415 (1990) Report on a Joint FAO/WHO Expert Consolation on Protein Quality Evaluation, , Bethesda Henley, E.C., Kuster, J.M., Protein quality evaluation by protein digestibility-corrected amino scoring (1994) Food Technol, 48 (4), pp. 74-77 Reeves, P.G.P.G., Nielsen, F.H., Fahey, G.N., AIN-93. Purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc Writing Committee on the formulation of the AIN-76. Rodent diet (1993) Nutrition, 123 (11), pp. 467-472 Sgarbieri, V.C., (1987) Alimentação e Nutrição: Fator de Saúde e Desenvolvimento, , São Paulo Almed Gomes, F.P., (1982) Curso de Estatística Experimental. 10. Ed., , São Paulo: Nobel Fox, P.F., McSweeney, P.L.H., Milk proteins (1998) Dairy Chemistry and Biochemistry, pp. 147-238. , London: Blackie Academic Friedman, M., Nutritional value of proteins from different food sources. A review (1996) J Agric Food Chem, 44 (1), pp. 6-29