Artículos de revistas
Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada
Isolation of polymers from Saccahromyces cerevisiae cell wall and evaluation of antioxidant activity of isolated mannan-protein
Registro en:
Química Nova. Sociedade Brasileira de Química, v. 32, n. 2, p. 322-326, 2009.
0100-4042
S0100-40422009000200010
10.1590/S0100-40422009000200010
Autor
Ferracini-Santos, Luciana
Sato, Hélia Harumi
Institución
Resumen
Yeast cell wall contains polymers glucan and mannan-protein that have received much attention with respect to their biological activities. Conventional isolation process involving treatments with hot alkali and acids cause degradation of these polymers. The aim of this paper was to study a low-degrading process for the isolation of glucan and mannan-protein from S. cerevisiae cell wall comprising physic and enzymatic treatments. Yeast cell glucan was obtained in a purity of 87.4% and a yield of 33.7%. The isolated mannan-protein presented antioxidant activity that was increased after thirty minutes of protease treatment. Antioxidant activity was determined by β-carotene/linoleate model system. 322 326 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)