Brasil
| Artículos de revistas
Low expression of APAF-1XL in acute myeloid leukemia may be associated with the failure of remission induction therapy
dc.creator | Benites, B.D. | |
dc.creator | Fattori, A. | |
dc.creator | Hackel, C. | |
dc.creator | Lorand-Metze, I. | |
dc.creator | De Souza, C.A. | |
dc.creator | Schulz, E. | |
dc.creator | Costa, F.F. | |
dc.creator | Saad, S.T.O. | |
dc.date | 2008-07-01 | |
dc.date | 2014-07-18T13:28:45Z | |
dc.date | 2015-11-26T11:39:42Z | |
dc.date | 2014-07-18T13:28:45Z | |
dc.date | 2015-11-26T11:39:42Z | |
dc.date.accessioned | 2018-03-28T20:43:06Z | |
dc.date.available | 2018-03-28T20:43:06Z | |
dc.identifier | Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 41, n. 7, p. 571-578, 2008. | |
dc.identifier | 0100-879X | |
dc.identifier | S0100-879X2008000700004 | |
dc.identifier | 10.1590/S0100-879X2008000700004 | |
dc.identifier | http://dx.doi.org/10.1590/S0100-879X2008000700004 | |
dc.identifier | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700004 | |
dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/31242 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/31242 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1234281 | |
dc.description | Apoptotic protease activating factor 1 (APAF-1) has a critical role in the regulation of apoptosis. In the present study, the mRNA expression analysis of different APAF-1 transcripts (APAF-1S, APAF-1LC, APAF-1LN, and APAF-1XL) was analyzed in bone marrow samples from 37 patients with acute myeloid leukemia (newly diagnosed, with no previous treatment). APAF-1XL and APAF-1LN transcripts (with and without an extra WD-40 repeat region, respectively) were detected in all samples, although the major form expressed was APAF-1XL in 65% of the samples (group 1), while 35% of the samples expressed primarily APAF-1LN (group 2). Only 46% of the patients presented complete remission in response to remission induction therapy (represented by less than 5% marrow blasts and hematological recovery), all but 2 cases being from group 1, 21.6% did not attain complete remission (only 1 case from group 1), and 32.4% of the patients died early. Lower expression of APAF-1XL (APAF-1XL/APAF-1LN ratio <1.2) was associated with a poor response to therapy (P = 0.0005, Fisher exact test). Both groups showed similar characteristics regarding white blood cell counts, cytogenetic data or presence of gene rearrangements associated with good prognosis as AML1-ETO, CBFB-MYH11 and PML/RARA. Since it has been shown that only the isoforms with the extra WD-40 repeat region activate procaspase-9, we suggest that low procaspase-9 activation may also be involved in the deregulation of apoptosis and chemotherapy resistance in acute myeloid leukemia. | |
dc.description | 571 | |
dc.description | 578 | |
dc.language | en | |
dc.publisher | Associação Brasileira de Divulgação Científica | |
dc.relation | Brazilian Journal of Medical and Biological Research | |
dc.rights | aberto | |
dc.source | SciELO | |
dc.subject | APAF-1 | |
dc.subject | Acute myeloid leukemia | |
dc.subject | Apoptosis | |
dc.subject | Chemotherapy resistance | |
dc.title | Low expression of APAF-1XL in acute myeloid leukemia may be associated with the failure of remission induction therapy | |
dc.type | Artículos de revistas |