PCR-based ribotyping of Staphylococcus aureus

dc.creatorOliveira, A.M.
dc.creatorRamos, M.C.
dc.date2002-02-01
dc.date2014-07-17T15:05:01Z
dc.date2015-11-26T11:25:25Z
dc.date2014-07-17T15:05:01Z
dc.date2015-11-26T11:25:25Z
dc.date.accessioned2018-03-28T20:39:24Z
dc.date.available2018-03-28T20:39:24Z
dc.identifierBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 2, p. 175-180, 2002.
dc.identifier0100-879X
dc.identifierS0100-879X2002000200005
dc.identifier10.1590/S0100-879X2002000200005
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2002000200005
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200005
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/24189
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/24189
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1233341
dc.descriptionGenotyping techniques are valuable tools for the epidemiologic study of Staphylococcus aureus infections in the hospital setting. Pulsed-field gel electrophoresis (PFGE) is the current method of choice for S. aureus strain typing. However, the method is laborious and requires expensive equipment. In the present study, we evaluated the natural polymorphism of the genomic 16S-23S rRNA region for genotyping purpose, by PCR-based ribotyping. Three primer pairs were tested to determine the size of amplicons produced and to obtain better discrimination with agar gel electrophoresis and ethidium bromide staining. The resolution of the typing system was determined using sets of bacteria obtained from clinical specimens from a large tertiary care hospital. These included DNA from three samples obtained from a bacteremic patient, six strains with known and diverse PGFE patterns, and 88 strains collected over a 3-month period in the same hospital. Amplification patterns obtained from samples from the same patient were identical, and PFGE from samples known to be different produced three genotypes. Amplification of DNA from 61 methicillin-resistant isolates produced only one pattern. Methicillin-sensitive strains yielded a diversity of patterns, pointing to a true polyclonal distribution throughout the hospital (22 unique patterns from 27 strains). Computer-based software can be used to differentiate among identifiable strains, given the low number of bands and good characterization of PCR products. PCR-based ribotyping can be a useful technique for genotyping methicillin-sensitive S. aureus strains, but is of limited value for methicillin-resistant strains.
dc.description175
dc.description180
dc.languageen
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relationBrazilian Journal of Medical and Biological Research
dc.rightsaberto
dc.sourceSciELO
dc.subjectStaphylococcus aureus
dc.subjectPCR ribotyping
dc.subjectGenotyping
dc.subjectMethicillin-resistant Staphylococcus aureus
dc.titlePCR-based ribotyping of Staphylococcus aureus
dc.typeArtículos de revistas


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