Tesis
Determinación de la actividad antimicrobiana del extracto etanólico y subextractos etéreo y clorofórmico de Duranta tricanta Juss, Callistemon speciosus y Tagetes minuta L.
Fecha
2013-07-01Registro en:
Uvidia Ortiz, Rosa Ana. (2013). Determinación de la actividad antimicrobiana del extracto etanólico y subextractos etéreo y clorofórmico de Duranta tricanta Juss, Callistemon speciosus y Tagetes minuta L. Escuela Superior Politécnica de Chimborazo. Riobamba.
Autor
Uvidia Ortiz, Rosa Ana
Resumen
The determination of the antimicrobial activity of ethanolic extracts and ethereal and chloroformic subextracts of Duranta triacanta, Callistemon speciosus and Tagetes minuta was carried out in the Phytochemistry and Microbiology Laboratories of the Faculty of Sciences of ESPOCH. Ethanolic extracts were obtained by maceration of the whole plant and were concentrated in rotavapor. Sub-extracts were extracted in a separating funnel with an aliquot of the ethanolic extract and immiscible ethyl ether, the upper phase being the ether. The same was done with chloroform, the lower phase is chloroform; the solvents were eliminated and the subextracts were obtained. The presence of essential oils, flavonoids and terpenoids in the ethanolic extracts was verified by coloring or precipitation reactions. The antimicrobial activity of the extracts against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 9637, Salmonella gallinarum ATCC 9184, Klebsiella pneumoniae ATCC 10031, A andida albicans ATCC 10231 and Pseudomonas aeruginosa ATCC 27853 was determined using Mitscher's method. At 10,000 µg/mL, the ethanolic extracts of the plants studied show activity against S. aureus and C. albicans, and only Callistemon speciosus is partially active against E. coli, S. gallinarun, K. pneumoniae and P. aeruginosa; at 1,000µg/mL the ethanolic extract of Callistemon speciosus is active against S. aureus and C. albicans. The ethereal subextracts of Tzinso and Cepillo inhibit S. aureus and C. albicans, the chloroformic subextract of Cepillo is partially active for S. gallinarum and K. pneumoniae. It was found that the ethanolic extracts and ethereal and chloroformic subextracts of the species studied do show antimicrobial activity, so it is recommended to continue with the studies to determine other biological activities such as anti-inflammatory, cholesterolemic, among others.