Now showing items 1-10 of 2301
Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells
(BioMed Central Ltd., 2019)
Background: The production of recombinant proteins in mammalian cell lines is one of the most important areas in biopharmaceutical industry. Viral transcriptional promoters are widely used to express recombinant proteins ...
T Cell Response of Asymptomatic Leishmania chagasi Infected Subjects to Recombinant Leishmania Antigens
(Mem??rias do Instituto Oswaldo Cruz, 1999)
Transcription factor engineering in CHO cells for recombinant protein production
(Taylor and Francis Ltd, 2019)
The continuous increase of approved biopharmaceutical products drives the development of more efficient recombinant protein expression systems. Chinese hamster ovary (CHO) cells are the mainstay for this purpose but have ...
Human interferon B1 ser17: Coding DNA synthesis, expression, purification and characterization of bioactive recombinant protein
A protocol to produce large amounts of bioactive homogeneous human interferon β1 expressed in Escherichia coli was developed. Human interferon β1 ser17 gene was constructed, cloned and subcloned, and the recombinant protein ...
Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compounds
BACKGROUND: Green fluorescent protein (GFP) is used extensively as a biomarker due to its unique spectral and fluorescence characteristics. GFP is usually obtained from recombinant strains of Escherichia coli (E. coli) ...
SK-HEP cells and lentiviral vector for production of human recombinant factor VIII
Hemophilia A is caused by a deficiency in coagulation factor VIII. Recombinant factor VIII can be used as an alternative although it is unavailable for most patients. Here, we describe the production of a human recombinant ...
NUCEL (Cell and Molecular Therapy Center): A multidisciplinary center for translational research in Brazil
(HUMANA PRESS INC, 2008)
Social and economical development is closely associated with technological innovation and a well-developed biotechnological industry. In the last few years, Brazil`s scientific production has been steadily increasing; ...
RECOMBINATION AS A POSSIBLE MAJOR CAUSE OF GENETIC-HETEROGENEITY IN ANTICARSIA-GEMMATALIS NUCLEAR POLYHEDROSIS-VIRUS WILD POPULATIONS
(Elsevier Science BvAmsterdamHolanda, 1992)