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Analytical and Clinical Validation for RT-qPCR Detection of SARS-CoV-2 Without RNA Extraction
(2020)
Background: The recent COVID-19 pandemic has posed an unprecedented challenge to laboratory diagnosis, based on the amplification of SARS-CoV-2 RNA. With global contagion figures exceeding 4 million persons, the shortage ...
Analytical and Clinical Validation for RT-qPCR Detection of SARS-CoV-2 Without RNA Extraction
(Frontiers Media, 2020)
Background: The recent COVID-19 pandemic has posed an unprecedented challenge to laboratory diagnosis, based on the amplification of SARS-CoV-2 RNA. With global contagion figures exceeding 4 million persons, the shortage ...
Impact of extracellular nucleic acids from lactic acid bacteria on qPCR and RT-qPCR results in dairy matrices: Implications for defining molecular markers of cell integrity
(Elsevier Science, 2017-07)
In this study, we evaluated whether quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) results could be affected by extracellular (free) DNA or RNA, respectively, obtained either from isolated bacteria or ...
Use of RNA-seq data to identify and validate RT-qPCR reference genes for studying the tomato-Pseudomonas pathosystem
(Nature Publishing Group, 2017-03)
The agronomical relevant tomato-Pseudomonas syringae pv. tomato pathosystem is widely used to explore and understand the underlying mechanisms of the plant immune response. Transcript abundance estimation, mainly through ...
Implementación de un protocolo para la detección de virus dengue por RT-qPCR en muestras biológicas
(BiologíaUniversidad El BosqueFacultad de Ciencias, 2022)
El dengue es una enfermedad tropical causada por un virus perteneciente a la familia Flaviviridae, es transmitido principalmente por artrópodos hematófagos especialmente por la picadura de mosquitos hembra de la especie ...