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Embryo aggregation does not improve the development of interspecies somatic cell nuclear transfer embryos in the horse
(Elsevier Science Inc, 2016-09)
The low efficiency of interspecies somatic cell nuclear transfer (iSCNT) makes it necessary to investigate new strategies to improve embryonic developmental competence. Embryo aggregation has been successfully applied to ...
Obtaiment of pudu (Pudu pudu) deer embryos by the somatic nuclear transfer technique
(Universidad de la Frontera, 2006)
SUMMARY: The objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu ...
Loss of Methylation at H19 DMD Is Associated with Biallelic Expression and Reduced Development in Cattle Derived by Somatic Cell Nuclear Transfer
(SOC STUDY REPRODUCTION, 2011)
Although cloning of mammals has been achieved successfully, the percentage of live offspring is very low because of reduced fetal size and fewer implantation sites. Recent studies have attributed such pathological conditions ...
Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos
(Public Library of Science, 2014-09)
Reproductive biotechnologies such asin vitrofertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improvedreproductive efficiency of animals. However, the birth rate ofin vitro-derived embryos still lags ...
Replication of somatic micronuclei in bovine enucleated oocytes
(BioMed Central, 2012-11)
BACKGROUND: Microcell-mediated chromosome transfer (MMCT) was developed to introduce a low number of chromosomes into a host cell. We have designed a novel technique combining part of MMCT with somatic cell nuclear transfer, ...
Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique
(Sociedad Chilena de Anatomía, 2006)
Imprinted gene expression in in vivo- And in vi/ro-produced bovine embryos and chorio-allantoic membranes
(Funpec-editora, 2009-06-15)
Cloning by nuclear transfer is often associated with poor results due to abnormal nuclear reprogramming of somatic donor cells and altered gene expression patterns. We investigated the expression patterns of imprinted genes ...
Produção de animais transgênicos por transferência nuclear como modelo de estudo biológico
(Belo Horizonte, 2008)
O recente progresso na clonagem animal por transferência nuclear (TN) possibilitou a produção de animais transgênicos utilizando linhagens de células doadoras de núcleo modificadas geneticamente. A possibilidade de manipulação ...