Now showing items 1-10 of 1064
SNP identification and validation on genomic DNA for studying genetic diversity in Thunnus albacares and Scomberomorus brasiliensis by combining RADseq and long read high throughput sequencing
A combination of a RADseq method (ddRAD) with long read high throughput sequencing (Roche 454) was tuned up in order to identify and validate a set of SNPs useful for gene diversity analysis in two important South American ...
Cetobacterium is a major component of the microbiome of giant amazonian fish (Arapaima gigas) in Ecuador
Arapaima gigas is a large air-breathing fish found in Amazonian rivers, a characteristic that gives this species an advantage in oxygen-deprived waters. It shows high potential for aquaculture in the Amazon region due ...
High-throughput cloning, expression and purification of glycoside hydrolases using Ligation-Independent Cloning (LIC)
Recent advances in DNA sequencing techniques have led to an explosion in the amount of available genome sequencing data and this provided an inexhaustible source of uncharacterized glycoside hydrolases (GH) to be studied ...
Transcriptomic crosstalk between fungal invasive pathogens and their host cells: Opportunities and challenges for next-generation sequencing methods
Fungal invasive infections are an increasing health problem. The intrinsic complexity of pathogenic fungi and the unmet clinical need for new and more effective treatments requires a detailed knowledge of the infection ...
High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.)
(Frontiers Media, 2020-09-11)
Alfalfa (Medicago sativa L.), also known as lucerne, is a major forage crop worldwide. In the United States, it has recently become the third most valuable field crop, with an estimated value of over $9.3 billion. Alfalfa ...
Exploring diversity and biotechnological potential of lactic acid bacteria from tocosh - traditional peruvian fermented potatoes - by high throughput sequencing (HTS) and culturing
Lactic acid bacteria (LAB) diversity associated with tocosh, Peruvian traditional fermented potatoes, was for the first time investigated by culturing and high throughput sequencing (HTS) approaches. They were applied on ...