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Aqueous Biphasic Systems Composed of Cholinium Chloride and Polymers as Effective Platforms for the Purification of Recombinant Green Fluorescent Protein
(2018-07-02)
Green fluorescent protein (GFP) has excellent properties as a biosensor and biomarker; however, its widespread use is limited by its purification costs. Alternative low-cost purification techniques can overcome this issue. ...
Protein refolding at high pressure: Optimization using eGFP as a model
(Elsevier Sci LtdOxfordInglaterra, 2011)
Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compounds
(2018-07-01)
BACKGROUND: Green fluorescent protein (GFP) is used extensively as a biomarker due to its unique spectral and fluorescence characteristics. GFP is usually obtained from recombinant strains of Escherichia coli (E. coli) ...
Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein
(Korean Soc Parasitology, Seoul Natl Univ Coll Medi, 2011-12-01)
Various Leishmania species were engineered with green fluorescent protein (GFP) using episomal vectors that encoded an antibiotic resistance gene, such as aminoglycoside geneticin sulphate (G418). Most reports of GFP-Leishmania ...
Investigation of charged polymer influence on green fluorescent protein thermal stability
(ELSEVIER SCIENCE BV, 2011)
Methods of stabilization and formulation of proteins are important in both biopharmaceutical and biocatalysis industries. Polymers are often used as modifiers of characteristics of biological macromolecules to improve the ...
Effect of Polyethylene Glycol on the Thermal Stability of Green Fluorescent Protein
(JOHN WILEY & SONS LTDInglaterra, 2010)
Fluorometric quantification of green fluorescent protein in tobacco leaf extracts
(Academic Press Inc Elsevier ScienceSan DiegoEUA, 2009)
Economic analysis of the production and recovery of green fluorescent protein using ATPS-based bioprocesses
(Elsevier B.V., 2021-01-01)
Green fluorescent protein (GFP) is a useful biomolecule in biotechnology; however, its price makes its widespread application prohibitive. To overcome this issue, recently, the use of aqueous two-phase systems (ATPS) for ...
Visualization and Functional Analysis of a Maxi-K Channel (mSlo) Fused to Green Fluorescent Protein (GFP)
(Pontificia Universidad Católica de Valparaíso, 1999)