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FAD binding properties of a cytosolic version of Escherichia coli NADH dehydrogenase-2
(Elsevier Science, 2014-03)
Respiratory NADH dehydrogenase-2 (NDH-2) of Escherichia coli is a peripheral membrane-bound flavoprotein. By eliminating its C-terminal region, a water soluble truncated version was obtained in our laboratory. Overall ...
Imaging transcription factors dynamics with advanced fluorescence microscopy methods
(Elsevier Science, 2018-12)
Pluripotent stem cells (PSCs) are capable of self-renewing and producing all cell types derived from the three germ layers in response to developmental cues, constituting an important promise for regenerative medicine. ...
Solvent-Tuned Supramolecular Assembly of Fluorescent Catechol/Pyrene Amphiphilic Molecules
(Wiley VCH Verlag, 2018-06-13)
The synthesis and structuration of a novel low‐molecular‐weight amphiphilic catechol compound is reported. The combination of a hydrophilic tail containing a catechol unit and a pyrene‐based hydrophobic head favors ...
Coherent control of single molecules at room temperature
(Royal Society of Chemistry, 2011-06)
The detection of individual molecules allows to unwrap the inhomogeneously broadened ensemble and reveal the spatial disorder and temporal dynamics of single entities. During 20 years of increasing sophistication this ...
Nucleus-cytoskeleton communication impacts on OCT4-chromatin interactions in embryonic stem cells
(BioMed Central, 2021-12)
Background: The cytoskeleton is a key component of the system responsible for transmitting mechanical cues from the cellular environment to the nucleus, where they trigger downstream responses. This communication is ...
Proton transfer from 2-naphthol to aliphatic amines in supercritical CO 2
(American Chemical Society, 2011-12)
The proton transfer from 2-naphthol to aliphatic amines was studied in supercritical CO 2 (scCO 2) and in cyclohexane as reference solvent, by absorption and fluorescence spectroscopy and by time-resolved emission. Irradiation ...
A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region
(Wiley VCH Verlag, 2018-04)
A novel fluorescent molecular probe is reported, which is able to detect glycoproteins, especially mucins, with high sensitivity and with a turn-on response along with a large Stokes shift (>130 nm), within the biologically ...
On line kinetic analysis of permeation profiles for UV filter loaded microemulsions using an automatic system with spectroscopic detection and a chemometric approach
(Royal Society of Chemistry, 2021-04-26)
The analysis of the permeation kinetics of new UV filter formulations is of great importance since the kinetic parameters are related to the effectiveness of the product over time. The dynamics of this process can be ...
Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells
(Elsevier Science, 2019-10)
Microtubule-dependent motors usually work together to transport organelles through the crowded intracellular milieu. Thus, transport performance depends on how motors organize on the cargo. Unfortunately, the lack of ...
Self-trapping of excitons, violation of condon approximation, and efficient fluorescence in conjugated cycloparaphenylenes
(American Chemical Society, 2014-11)
Cycloparaphenylenes, the simplest structural unit of armchair carbon nanotubes, have unique optoelectronic properties counterintuitive in the class of conjugated organic materials. Our time-dependent density functional ...