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The photoreversible fluorescent protein iLOV outperforms GFP as a reporter of plant virus infection
(National Academy of Sciences, 2008-12-16)
Fluorescent proteins (FPs) based on green fluorescent protein (GFP) are widely used throughout cell biology to study protein dynamics, and have extensive use as reporters of virus infection and spread. However, FP-tagging ...
Articaine interaction with phospholipid bilayers
(Elsevier Science, 2020-12)
Local anesthetics promote analgesia by interacting with excitable membranes. Articaine (ATC) has a unique composition among local anesthetics as it possesses a thiophene instead of the typical phenyl ring. Aiming to ...
Benzoxaboroles as dynamic covalent receptors for bioconjugation and transport of nucleosides and related drugs: proof of action in HeLa cells
(Academic Press Inc Elsevier Science, 2019-06)
In this work we describe not previously explored binding studies on the reversible interaction of benzoxaborole with ligands of medical and pharmaceutical interest such as nucleosidic drugs gemcitabine and capecitabine, ...
Dynamics of the dissolved organic matter in a stream-lake system within an extremely acid to neutral pH range: Agrio-Caviahue watershed
(Pergamon-Elsevier Science Ltd, 2020-07)
The Agrio-Caviahue is a natural stream-lake system composed of the Upper Agrio River (UAR) -Lake Caviahue- Lower Agrio River (LAR). The system has a broad pH gradient from extremely acid in the hot spring to almost neutral ...
Giant vesicles, laurdan, and two-photon fluorescence microscopy: Evidence of lipid lateral separation in bilayers
(Elsevier Academic Press Inc., 2003-03)
This chapter describes giant vesicles, Laurdan, and two-photon fluorescence microscopy. The combination of Laurdan, giant unilamellar vesicles (GUVs), and two-photon fluorescence microscopy has been extremely useful in ...
Fluorescent redox-dependent labeling of lipid droplets in cultured cells by reduced phenazine methosulfate
(Elsevier, 2020-06)
Natural and synthetic phenazines are widely used in biomedical sciences. In dehydrogenase histochemistry, phenazine methosulfate (PMS) is applied as a redox reagent for coupling reduced coenzymes to the reduction of ...
Fluorescence correlation spectroscopy experiments to quantify free diffusion coefficients in reaction-diffusion systems: The case of Ca 2 + and its dyes
(American Physical Society, 2017-06)
Many cell signaling pathways involve the diffusion of messengers that bind and unbind to and from intracellular components. Quantifying their net transport rate under different conditions then requires having separate ...
Dynamical reorganization of the pluripotency transcription factors Oct4 and Sox2 during early differentiation of embryonic stem cells
(Nature, 2020-03)
Pluripotency maintenance requires transcription factors (TFs) that induce genes necessary to preserve the undifferentiated state and repress others involved in differentiation. Recent observations support that the heterogeneous ...
Optical studies of single-tryptophan B. licheniformis β-lactamase variants
(Elsevier Science, 2010-10)
β-lactamases (penicillinases) are important complicating factors in bacterial infections and excellent theoretical and experimental models in protein structure, dynamics and evolution. Bacillus licheniformis exo-small ...
Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells
(IOP Publishing, 2018-01)
Most accepted single particle tracking methods are able to obtain high-resolution trajectories for relatively short periods of time. In this work we apply a straightforward combination of single-particle tracking microscopy ...